The transcription factor MYB43 antagonizes with ICE1 to regulate freezing tolerance in Arabidopsis

Abstract

Summary Previous discovering meticulously illustrates the post‐translational modifications and protein stability regulation of ICE1 and their role in cold stress. However, the studies on the interaction of ICE1 with other transcription factors, and their function in modulation cold stress tolerance, as well as in the transition between cold stress and growth are largely insufficient. In this work, we found that maltose binding protein (MBP) 43 directly binds to the promoters of CBF genes to repress their expression, thereby negatively regulating freezing tolerance. Biochemical and genetic analyses showed that MYB43 interacts and antagonizes with ICE1 to regulate the expression of CBF genes and plant's freezing stress tolerance. PLEIOTROPIC REGULATORY LOCUS 1 (PRL1) accumulates under cold stress and promotes MYB43 protein degradation; however, when cold stress disappears, PRL1 restores normal protein levels, causing MYB43 protein to re‐accumulate to normal levels. Furthermore, PRL1 positively regulates freezing tolerance by promoting degradation of MYB43 to attenuate its repression of CBF genes and antagonism with ICE1. Thus, our study reveals that MYB43 inhibits CBF genes expression under normal growth condition, while PRL1 promotes MYB43 protein degradation to attenuate its repression of CBF genes and antagonism with ICE1, and thereby to the precise modulation of plant cold stress responses.

Genome-wide association reveals novel insights into the molecular mechanisms regulating stem volume inPinus taeda

“…Another transcription factor exhibiting a high topological coefficient in our coexpression network was ICE1, which was previously linked to the regulation of cold response and freezing tolerance [106]. Similarly, our analysis identified the transcription factor SCREAM2, which is known for its influence on the successive initiation, proliferation, and terminal differentiation of stomatal cell lineages [107].…”

Genome-Wide Identification of G-Quadruplex forming regions in Arabidopsis: Unraveling the Role of Pif like Helicase (AtPLH1) in Gene Regulation and Stress Response

“…AP2/ERF and BZR transcription factors are known to regulate plant growth and development along with stress-responsive gene expression (Xie et al, 2019). MYB3 was shown to antagonize ICE1 to regulate CBF-mediated gene expression to regulate freezing stress tolerance along with role in lignin biosynthesis genes during secondary wall formation in Arabidopsis (Zheng et al, 2023, Geng et al, 2020). These results suggest that mutation in AtPLH1 alter ERF, brassinosteriod, JA and cold regulated gene expression and plant development.…”

“…Differential gene expression analysis suggested that MYB43 target genes are upregulated in Atplh1 . Further, MYB43 TF regulate freezing stress tolerance by antagonizing ICE1 transcription factor to regulate CBF gene expression in Arabidopsis (Zheng et al, 2023). Thus, we analyzed the response of Atplh1 mutants under cold stress at different temperatures (4°C, 1°C and -20°C).…”

Functions of Basic Helix–Loop–Helix (bHLH) Proteins in the Regulation of Plant Responses to Cold, Drought, Salt, and Iron Deficiency: A Comprehensive Review

“…Coldactivated PLEIOTROPIC REGULATORY LOCUS 1 (PRL1) mediates the ubiquitination and degradation of MYB43 to facilitate freezing tolerance. 196 Two PLANT U-BOX E3 ligases, PUB25 and PUB26, mediated K48-and K63-linked chains to ICE1 and MYB15 to dynamically modulate their stability at the different stages of cold stress. 197 Sumoylation, mediated by SUMO E3 ligases, represents another crucial pathway that protects proteins from degradation in response to stress.…”

“…Furthermore, stabilized MYB43 represses the transcriptional activity of CBF and antagonizes with ICE1. Cold-activated PLEIOTROPIC REGULATORY LOCUS 1 (PRL1) mediates the ubiquitination and degradation of MYB43 to facilitate freezing tolerance . Two PLANT U-BOX E3 ligases, PUB25 and PUB26, mediated K48- and K63-linked chains to ICE1 and MYB15 to dynamically modulate their stability at the different stages of cold stress …”