The transcriptional factor YB-1 positively regulates Hsc70 transcription in Crassostrea hongkongensis

Liu, Huiru; Xu, Delin; Cui, Miao; Hou, Ting-Long; Zhang, Qizhong

doi:10.1016/j.bbrc.2017.12.110

Cited by 9 publications

(3 citation statements)

References 31 publications

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“…ToElovl5 transcription indicated increasing profiles in either native TOCF cells or heterologous HEK 293T cells. These results were reasonable due to the stress caused by the disturbed biological environment during in vitro TOCF cell culture or Elovl5 promoter expression in the heterologous host ( Liu et al, 2018 ).…”

Section: Discussionmentioning

confidence: 55%

The Transcriptional Factor PPARαb Positively Regulates Elovl5 Elongase in Golden Pompano Trachinotus ovatus (Linnaeus 1758)

et al. 2018

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The nuclear peroxisome proliferator-activated receptors (PPARs) regulate the transcription of elongases of very long-chain fatty acids (Elovl), which are involved in polyunsaturated fatty acid (PUFA) biosynthesis in mammals. In the present study, we first characterized the function of Elovl5 elongase in Trachinotus ovatus. The functional study showed that ToElovl5 displayed high elongation activity toward C18 and C20 PUFA. To investigate whether PPARαb was a regulator of Elovl5, we also reported the sequence of T. ovatus PPARαb (ToPPARαb). The open reading frame (ORF) sequence encoded 469 amino acids possessing four typical characteristic domains, including an N-terminal hypervariable region, a DNA-binding domain (DBD), a flexible hinge domain and a ligand-binding domain (LBD). Thirdly, promoter activity experiments showed that the region from PGL3-basic-Elovl5-5 (-146 bp to +459 bp) was defined as the core promoter by progressive deletion mutation of Elovl5. Moreover, PPARαb overexpression led to a clear time-dependent enhancement of ToElovl5 promoter expression in HEK 293T cells. Fourth, the agonist of PPARαb prominently increased PPARαb and Elovl5 expression, while PPARαb depletion by RNAi or an inhibitor was correlated with a significant reduction of Elovl5 transcription in T. ovatus caudal fin cells (TOCF). In conclusion, the present study provides the first evidence of the positive regulation of Elovl5 transcription by PPARαb and contributes to a better understanding of the transcriptional mechanism of PPARαb in fish.

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Section: Discussionmentioning

confidence: 55%

The Transcriptional Factor PPARαb Positively Regulates Elovl5 Elongase in Golden Pompano Trachinotus ovatus (Linnaeus 1758)

et al. 2018

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“…The induced expression of heat shock proteins (HSPs) is a common response mechanism to various stresses, including oxidative (Amadio et al, ), ischemic (Kraynik et al, ), and thermal (Daugaard, Rohde, & Jäättelä, ) stress. The induction of these genes is tightly regulated, involving both transcriptional and posttranscriptional regulation (Daugaard et al, ; H. Liu, Xu, Cui, Hou, & Zhang, ; Morimoto, ). The most prominent member of the HSP family, HSP70, is tightly regulated and has many protein isoforms encoded by separate gene loci which have distinct regulatory characteristics allowing them to help cells respond to various types of assaults (Daugaard et al, ; Tranter et al, ).…”

Section: Apa In Cellular Responses To Stressmentioning

confidence: 99%

“…The induced expression of heat shock proteins (HSPs) is a common response mechanism to various stresses, including oxidative (Amadio et al, 2008), ischemic (Kraynik et al, 2015), and thermal (Daugaard, Rohde, & Jäättelä, 2007) stress. The induction of these genes is tightly regulated, involving both transcriptional and posttranscriptional regulation (Daugaard et al, 2007;H. Liu, Xu, Cui, Hou, & Zhang, 2017;Morimoto, 1998).…”

Section: Alternative Polyadenylation Of Hsp Genesmentioning

confidence: 99%

Alternative polyadenylation and the stress response

et al. 2019

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The cellular stress response is a universal mechanism necessary for the survival of all organisms. This multifaceted process is primarily driven by regulation of gene expression to produce an intracellular environment suitable for promoting cell survival and recovery. Posttranscriptional regulatory events are considered as critical mechanisms that modulate core characteristics of mRNA transcripts to promote cell adaptation to various assaults. While the impact of processes such as mRNA splicing, turnover, localization, and translation on the cellular stress response has been extensively studied, recent observations highlight the role of alternative polyadenylation (APA) in response to challenges such as oxidative stress, heat shock, and starvation. The role of APA is comprehensive with far reaching effects on mRNA stability, mRNA localization, and protein coding sequences. Nonetheless, APA remains a relatively unappreciated mode of gene regulation despite its role in regulating key mediators of the stress response. The goal of this review is to provide an overview of the recent advances in our understanding of the various ways by which APA affects cell adaptation to its environment and discuss how a defect in APA could have deleterious consequences on cell survival.

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Molecular characterization, expression following cold stress, and functional characterization of YB-1 gene in the spotted sea bass (Lateolabrax maculatus)

Wang

et al. 2022

Aquacult Int

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The transcriptional factor YB-1 positively regulates Hsc70 transcription in Crassostrea hongkongensis

Cited by 9 publications

References 31 publications

The Transcriptional Factor PPARαb Positively Regulates Elovl5 Elongase in Golden Pompano Trachinotus ovatus (Linnaeus 1758)

The Transcriptional Factor PPARαb Positively Regulates Elovl5 Elongase in Golden Pompano Trachinotus ovatus (Linnaeus 1758)

Alternative polyadenylation and the stress response

Molecular characterization, expression following cold stress, and functional characterization of YB-1 gene in the spotted sea bass (Lateolabrax maculatus)

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