The transcriptional regulator Zur regulates the expression of ZnuABC and T6SS4 in response to stresses in Yersinia pseudotuberculosis

Cai, Rong; Gao, Fen Biao; Pan, Junfeng; Hao, Xinwei; Yu, Zhengqing; Qu, Yichen; Li, Jialin; Wang, Dandan; Wang, Yao; Shen, Xihui; Liu, Xingyu; Yang, Yantao

doi:10.1016/j.micres.2021.126787

Cited by 17 publications

(10 citation statements)

References 65 publications

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“…The localization of the Zur boxes on the promoter region of its target genes, along with the ability of the protein to multimerize at high zinc concentrations, appear to determine whether the regulator functions as a repressor or an activator (6). Other processes, not directly related to metal homeostasis, have also been found to be positively regulated by Zur such as the synthesis of secondary metabolites in Streptomyces avermitilis or the type VI secretion system in Yersinia pseudotuberculosis (21,22). In the case of P. aeruginosa, we found that the Zur protein binds in a Zn-dependent manner to two DNA regions (Zur box1 and 2) located downstream of the czcR promoter.…”

Section: Discussionmentioning

confidence: 71%

A Zur-mediated transcriptional regulation of the zinc export system

Ducret

González

Leoni

et al. 2022

Preprint

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The control of cellular zinc (Zn) concentrations by dedicated import and export systems is essential for the survival and virulence of Pseudomonas aeruginosa. The transcription of its many Zn transporters is therefore tightly regulated by a known set of transcription factors involved in either the import or the export of Zn. In this work, we show that the Zur protein, a well-known repressor of Zn import, plays a dual role and functions in both import and export processes. In a situation of Zn excess, Zur represses Zn entry, but also activates the transcription of czcR, a positive regulator of the Zn export system. To achieve this, Zur binds at two sites, located by DNA footprinting in the region downstream the czcR transcription start site. In agreement with this regulation, a delay in induction of the efflux system is observed in the absence of Zur and Zn resistance is affected. The discovery of this regulation highlights a new role of Zur as global regulator of Zn homeostasis in P. aeruginosa disclosing an important link between Zur and zinc export.

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Section: Discussionmentioning

confidence: 71%

A Zur-mediated transcriptional regulation of the zinc export system

Ducret

González

Leoni

et al. 2022

Preprint

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“…Consistent with the function of T6SS-4 in combating stress, its expression is regulated by multiple transcription regulators, such as OmpR (Gueguen et al, 2013;Zhang et al, 2013), OxyR (Wang et al, 2015), RovM (Song et al, 2015), RpoS (Guan et al, 2015), and RelA (Yang et al, 2019), all of which respond to various stresses (Song et al, 2015;Zhao et al, 2017;Yang et al, 2019). Both ZntR and Zur, two zinc responsive regulators, are also involved in T6SS-4 regulation by directly binding to its promoter region (Wang et al, 2017;Cai et al, 2021).…”

Section: Zincmentioning

confidence: 83%

Roles of Type VI Secretion System in Transport of Metal Ions

Yang

Liu²,

Zhang³

et al. 2021

Front. Microbiol.

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The type VI secretion system (T6SS) is a transmembrane protein nanomachine employed by many gram-negative bacteria to directly translocate effectors into adjacent cells or the extracellular milieu, showing multiple functions in both interbacterial competition and bacteria-host interactions. Metal ion transport is a newly discovered T6SS function. This review summarizes the identified T6SS functions and highlights the features of metal ion transport mediated by T6SS and discusses its regulation.

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“…As Zn concentration increases, Zur multimerizes on the promoter and increases zitB transcription [ 23 ]. Zur also acts as a positive transcriptional regulator of T6SS4 in Yersinia pseudotuberculosis by binding to the -90 DNA region [ 24 ]. This secretion system exports YezP, a Zn binding protein implicated in oxidative stress responses [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

A Zur-mediated transcriptional regulation of the zinc export system in Pseudomonas aeruginosa

et al. 2023

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The control of cellular zinc (Zn) concentrations by dedicated import and export systems is essential for the survival and virulence of Pseudomonas aeruginosa. The transcription of its many Zn transporters is therefore tightly regulated by a known set of transcription factors involved in either the import or the export of Zn. In this work, we show that the Zur protein, a well-known repressor of Zn import, plays a dual role and functions in both import and export processes. In a situation of Zn excess, Zur represses Zn entry, but also activates the transcription of czcR, a positive regulator of the Zn export system. To achieve this, Zur binds at two sites, located by DNA footprinting in the region downstream the czcR transcription start site. In agreement with this regulation, a delay in induction of the efflux system is observed in the absence of Zur and Zn resistance is reduced. The discovery of this regulation highlights a new role of Zur as global regulator of Zn homeostasis in P. aeruginosa disclosing an important link between Zur and zinc export.

show abstract

The transcriptional regulator Zur regulates the expression of ZnuABC and T6SS4 in response to stresses in Yersinia pseudotuberculosis

Cited by 17 publications

References 65 publications

A Zur-mediated transcriptional regulation of the zinc export system

A Zur-mediated transcriptional regulation of the zinc export system

Roles of Type VI Secretion System in Transport of Metal Ions

A Zur-mediated transcriptional regulation of the zinc export system in Pseudomonas aeruginosa

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