The transcriptional regulatory network of Corynebacterium jeikeium K411 and its interaction with metabolic routes contributing to human body odor formation

Barzantny, Helena; Schröder, Jasmin; Strotmeier, Jasmin; Fredrich, Eugenie; Brune, Iris; Tauch, Andreas

doi:10.1016/j.jbiotec.2012.01.021

Cited by 18 publications

(27 citation statements)

References 117 publications

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“…[44,52] Additionally,t here have been numerous studies on the influence of microbial biota on skin VOCs,w hich, in turn, can vary greatly among human subjects. [46,53] Of the different areas of the skin, the axillary region is probably the most important. VOCs from axillae generally consist of alkanes and C 6 -C 11 carboxylic acids.…”

Section: Voc Emission From Blood To Skinmentioning

confidence: 99%

Hybrid Volatolomics and Disease Detection

et al. 2015

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This Review presents a concise, but not exhaustive, didactic overview of some of the main concepts and approaches related to "volatolomics"-an emerging frontier for fast, risk-free, and potentially inexpensive diagnostics. It attempts to review the source and characteristics of volatolomics through the so-called volatile organic compounds (VOCs) emanating from cells and their microenvironment. It also reviews the existence of VOCs in several bodily fluids, including the cellular environment, blood, breath, skin, feces, urine, and saliva. Finally, the usefulness of volatolomics for diagnosis from a single bodily fluid, as well as ways to improve these diagnostic aspects by "hybrid" approaches that combine VOC profiles collected from two or more bodily fluids, will be discussed. The perspectives of this approach in developing the field of diagnostics to a new level are highlighted.

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Section: Voc Emission From Blood To Skinmentioning

confidence: 99%

Hybrid Volatolomics and Disease Detection

et al. 2015

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“…In addition to its role in acetate metabolism, glxR is involved in many other cellular activities, such as carbon metabolism [6], [11]–[14], energy metabolism [15], lipid metabolism [16], anaerobic nitrate respiration [17], inorganic phosphate uptake [18], and cell resuscitation [19]. Further, Kohl et al [20] analyzed the whole genome using in silico tools in combination with in vitro analysis and predicted that the GlxR regulon comprises many genes (96 genes in 53 transcription units) presumed to be involved in diverse cellular activities such as carbohydrate metabolism, aromatic compound degradation, glutamate uptake and nitrogen assimilation, fatty acid biosynthesis, stress response, and resuscitation.…”

Section: Introductionmentioning

confidence: 99%

Role of Corynebacterium glutamicum sprA Encoding a Serine Protease in glxR-Mediated Global Gene Regulation

et al. 2014

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The global regulator glxR of Corynebacterium glutamicum is involved in many cellular activities. Considering its role, the GlxR protein likely interacts with other proteins to obtain, maintain, and control its activity. To isolate proteins interacting with GlxR, we used a two-hybrid system with GlxR as the bait. Subsequently, the partner, a subtilisin-like serine protease, was isolated from a C. glutamicum genomic library. Unlike glxR, which showed constitutive expression, the expression of sprA, encoding a serine protease, was maximal in the log phase. Purified His6-SprA protein underwent self-proteolysis and proteolyzed purified GlxR. The proteolytic action of SprA on GlxR was not observed in the presence of cyclic adenosine monophosphate, which modulates GlxR activity. The C. glutamicum sprA deletion mutant (ΔsprA) and sprA-overexpressing (P180-sprA) strains showed reduced growth. The activity of isocitrate dehydrogenase (a tricarboxylic acid cycle enzyme) in these strains decreased to 30–50% of that in the wild-type strain. In the P180-sprA strain, proteins involved in diverse cellular functions such as energy and carbon metabolism (NCgl2809), nitrogen metabolism (NCgl0049), methylation reactions (NCgl0719), and peptidoglycan biosynthesis (NCgl1267), as well as stress, starvation, and survival (NCgl0938) were affected and showed decreased transcription. Taken together, these data suggest that SprA, as a serine protease, performs a novel regulatory role not only in glxR-mediated gene expression but also in other areas of cell physiology. In addition, the tight control of SprA and GlxR availability may indicate their importance in global gene regulation.

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“…The detected binding of the regulatory proteins GlxR and Jk0257 to the fadE6 upstream region proposes the presence of global and local control mechanisms for fadE6 transcription. Furthermore, the prediction of a GlxR binding site in the jk0257 upstream region (Barzantny et al ., ) suggests the co‐regulation of fadE6 by a hierarchical network structure. To verify the binding of the global regulator GlxR to the jk0257 upstream region, a DNA affinity chromatography experiment was carried out.…”

Section: Resultsmentioning

confidence: 97%

“…). Strikingly, the regulator is only encoded in sequenced genomes of the clusters 3 and 4 of the genus Corynebacterium , comprising many species that are characterized by a lipid‐dependent lifestyle or reside in lipid‐rich habitats (Khamis et al ., ; Barzantny et al ., ). Generally, MarR‐like regulators control diverse cellular processes, where they function as either activator or repressor (Perera and Grove, ).…”

Section: Resultsmentioning

confidence: 99%

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Transcriptional control of lipid metabolism by the MarR‐like regulator FamR and the global regulator GlxR in the lipophilic axilla isolate Corynebacterium jeikeium K411

Barzantny

Guttmann

Lässig

et al. 2012

Microbial Biotechnology

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Corynebacterial fatty acid metabolism has been associated with human body odour, and is therefore discussed as a potential target for the development of new deodorant additives. For this reason, the transcription levels of fad genes associated with lipid metabolism in the axilla isolate Corynebacterium jeikeium were analysed during growth on different lipid sources. The transcription of several fad genes was induced two-to ninefold in the presence of Tween 60, including the acyl-CoA dehydrogenase gene fadE6. DNA affinity chromatography identified the MarR-like protein FamR as candidate regulator of fadE6. DNA band shift assays and in vivo reporter gene fusions confirmed the direct interaction of FamR with the mapped fadE6 promoter region. Moreover, DNA affinity chromatography and DNA band shift assays detected the binding of GlxR to the promoter regions of fadE6 and famR, revealing a hierarchical control of fadE6 transcription by a feed-forward loop. Binding of GlxR and FamR to additional fad gene regions was demonstrated in vitro by DNA band shift assays, resulting in the co-regulation of fadA, fadD, fadE and fadH genes. These results shed first light on the hierarchical transcriptional control of lipid metabolism in C. jeikeium, a pathway associated with the development of human axillary odour.Funding Information H. B. acknowledges the scholarship granted by the CLIB-Graduate Cluster Industrial Biotechnology co-financed by the Ministry of Innovation, Science, and Research of North Rhine Westphalia. The authors thank Benjamin Müller (CeBiTec) for expert help with the MALDI-TOF/TOF mass spectrometer and Christian Ziert (Bielefeld University) for valuable advice concerning the gfp reporter fusion.

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The transcriptional regulatory network of Corynebacterium jeikeium K411 and its interaction with metabolic routes contributing to human body odor formation

Cited by 18 publications

References 117 publications

Hybrid Volatolomics and Disease Detection

Hybrid Volatolomics and Disease Detection

Role of Corynebacterium glutamicum sprA Encoding a Serine Protease in glxR-Mediated Global Gene Regulation

Transcriptional control of lipid metabolism by the MarR‐like regulator FamR and the global regulator GlxR in the lipophilic axilla isolate Corynebacterium jeikeium K411

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