The transcriptome landscapes of allantochorion and vitelline-chorion in equine day 30 conceptus

Shen, Yingchao; Hong, Richang; Davshilt, Toli; Tian, Shuyue; Wang, Xisheng; Yi, Minna; Ulaangerel, Tseweendolmaa; Li, Bei; Dugarjav, Manglai; Bou, Germán

doi:10.3389/fcell.2022.958205

Cited by 1 publication

(2 citation statements)

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“…Nine genes (CYP11A1, CYP17A1, HSD17B7, LDLR, SCARB1, STAR, PLA1A, CPNE5 and COL4A4) that were found to be differentially expressed were selected for qPCR validation. Total RNA isolation, cDNA synthesis and qPCR analysis were conducted as previously described (Shen et al., 2022). The primers used for qPCR analysis are listed in Table S1, with GAPDH identified as a reference control.…”

Section: Methodsmentioning

confidence: 99%

“…The qPCRs were performed on the Bio‐Rad CFX96 Real‐Time PCR System (Bio‐Rad, USA) using SYBR Green qPCR Mix (Takara, CHN) according to the manufacturer's instructions. The thermal cycling conditions were as follows: 95°C for 5 min, followed by 35 cycles of 95°C for 1 min and 60°C for 30 s. Relative gene expression levels were calculated using the

{2}^{-\Delta \Delta {C}_t}

method (Shen et al., 2022).…”

Section: Methodsmentioning

confidence: 99%

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Transcriptome profiling of equine ovarian cortex during foetal and adult stages

Shen,

Davshilt,

Ren

et al. 2024

Reprod Domestic Animals

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The equine foetal ovary has some unique characteristics that differ from other animals, such as ovary hyperplasia and high oestrogen secretion, and inverted location of cortex and medulla present in adult mare. The ovarian cortex supports the process of folliculogenesis and development, the synthetics and expression of oestrogen; however, the character‐related molecular mechanisms and specific biological functions in equine foetal ovarian cortex are still poorly understood. To explore the associated regulatory networks and molecular events of equine foetal ovary during the hyperplasia stage, we analysed the transcriptomic differences between the equine second‐trimester foetal ovarian cortex and adult ovarian cortex by RNA‐seq. There were 6334 differentially expressed genes (DEGs) present in foetal vs. adult, of which 3234 and 3100 DEGs were upregulated and downregulated, respectively. GO and KEGG were used for functional and pathway enrichment analysis of the DEGs. In particular, we found that some DEGs with high fold changes were related to steroid production and metabolism, such as CYP11A1, CYP17A1, LDLR, STAR and SCARB1, which were in line with the efficient oestrogen production in the equine foetal ovary. The transcriptome profile revealed the functional and developmental characteristics and gene expression patten of the ovarian cortex in the foetal and adult stages of the mare. Our study provides new insights into the molecular mechanism of equine second‐trimester foetal ovarian development through transcriptome analysis and provides insights for improving care or treatment for the pregnant mare and the foetus.

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Section: Methodsmentioning

confidence: 99%