The transcriptome variations of Panaxnotoginseng roots treated with different forms of nitrogen fertilizers

Ou, Xiaohong; Li, Shipeng; Liao, Peiran; Cui, Xiuming; Zheng, Binglian; Yang, Ye; Liu, Dahui; Zheng, Yun

doi:10.1186/s12864-019-6340-7

Cited by 18 publications

(10 citation statements)

References 60 publications

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“…This result was consistent with the results such as wheat, cotton, and Camptotheca acuminate [15,36,37]. With the increase of N application rate, root and total N, P, and K accumulation first increased and then decreased, meaning it was possible that excessive N fertilizer would poison the roots of P. bournei seedlings and reduce the ability to absorb nutrients, resulting in N, P, K accumulation decrease [38,39]. With the increase of N application rate, leaves N and P allocation rate first decreased and then increased (opposition to root and stem), indicating that low-medium level N fertilization is more beneficial to root and stem growth, and high-level N fertilization is more beneficial to leaves growth.…”

Section: Effect Of N Fertilizer On Nutrients Accumulation and Allocation In P Bournei Seedlingssupporting

confidence: 90%

Fertilization Regulates Accumulation and Allocation of Biomass and Nutrients in Phoebe bournei Seedlings

Yang

Chen

et al. 2021

Agriculture

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To study the effect of N-P-K fertilization on Phoebe bournei seedlings’ organs dry biomass, and nutrients accumulation and allocation, and to further uncover how nutrients regulating dry biomass formation through fertilization, we utilized the “3414” experiment design. The results showed that N, P, and K fertilizer promoted dry biomass accumulation, and root, stem, and total plant N, P, and K content and accumulation in seedlings. The dry biomass accumulations of root, stem, and total plant increased first and then decreased with the increase of N, P, and K application rates, which was basically consistent with the change in dry biomass allocations and N, P, and K contents, accumulations, and allocations. Root N accumulation, root P accumulation, and total plant K accumulation were the key indicators for seedlings growth. N fertilizer had the greatest effect on total dry biomass and root N accumulation, was the most important fertilizer for the growth of Phoebe bournei seedlings, can regulate the growth of root and leaves, is beneficial to root growth at medium-low N fertilizer levels (N: 0.266–0.532 g·plant−1), and leaves growth at high N fertilizer level (N: 0.798 g·plant−1). P fertilizer rate can regulate the seedling stem growth, reaching the maximum at the medium level P application (P2O5: 0.1332 g·plant−1). K fertilizer had the greatest effect on the root P accumulation and total K accumulation, promoting K transport from leaves to root, improved root and stem growth, and inhibited leaves growth. The N, P, and K fertilizer three-factor application can better promote nutrient uptake than double-factor and single-factor fertilization, with highest dry biomass accumulation at the medium level of N, P, K fertilizer (N: 0.532 g·plant−1; P2O5: 0.1232 g·plant−1; K2O: 0.356 g·plant−1). In conclusion, N, P, and K fertilization promoted the N, P, and K absorption, increased root, stem, and leaves N, P, and K content and accumulation, and promoted the seedling dry biomass accumulation, but reversed under excessive application of N, P, and K fertilizer; and N fertilizer was beneficial to root and leaves growth, P fertilizer to stem growth, and K fertilizer to material transfer, which provided a theoretical basis for robust Phoebe bournei seedling cultivation.

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Section: Effect Of N Fertilizer On Nutrients Accumulation and Allocation In P Bournei Seedlingssupporting

confidence: 90%

Fertilization Regulates Accumulation and Allocation of Biomass and Nutrients in Phoebe bournei Seedlings

Yang

Chen

et al. 2021

Agriculture

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“…During N assimilation in plants, the NH 4 + is incorporated into amino acids with the action of NH 4 + assimilation enzymes including glutamine synthetase (GS), glutamate synthase (GOGAT) and glutamate dehydrogenase (GDH), and then converted into protein. 9 , 18 , 36 In a previous work, we found that NO 3 − addition increased the activity of GS, GOGAT and GDH under NH 4 + toxicity. 21 Therefore, we measured protein content of wheat roots under different N treatments.…”

Section: Discussionmentioning

confidence: 89%

“… 17 It was found that nitrate alleviated the toxic effect of NH 4 + on root growth of Panax notoginseng by up-regulation of ACLA-3 (encoding ATP-citrate lyase A-3) and several key metabolites in the tricarboxylic acid (TCA) cycle. 18 SLAH3, a slow-type NO 3 − efflux channel, mediates the NO 3 − efflux to buffer the low cellular pH caused by NH 4 + uptake. 6 SnRK1.1 can regulate SLAH3 and thereby mediate NO 3 − efflux, leading to alleviation of high-NH 4 + /low-pH stress.…”

Section: Introductionmentioning

confidence: 99%

Nitrate alleviates ammonium toxicity in wheat (Triticum aestivumL.) by regulating tricarboxylic acid cycle and reducing rhizospheric acidification and oxidative damage

Zhang

et al. 2021

Plant Signaling & Behavior

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Ammonium (NH 4 + ) is one of the most important nutrients required by plants. However, a high concentration of NH 4 + as the sole nitrogen source suppresses plant growth. Although nitrate (NO 3 − ) can alleviate NH 4 + toxicity, the mechanisms underlying this ability have not been fully elucidated. In this study, wheat plants were cultivated in hydroponic solution with 7.5 mM NO 3 − (control), 7.5 mM NH 4 + (sole ammonium, SA) or 7.5 mM NH 4 + plus 1.0 mM NO 3 − (ammonium and nitrate, AN). The results showed that compared with the control, the SA treatment significantly decreased root growth, protein content and the concentrations of most intermediates and the activity of enzymes from the tricarboxylic acid (TCA) cycle. Moreover, increased the activity of plasma membrane H + -ATPase and the rate of H + efflux along roots, caused solution acidification, and increased the activity of mitochondrial respiratory chain complexes I–IV and the contents of protein-bound carbonyls and malondialdehyde in roots. SA treatment induced ultrastructure disruption and reduced the viability of root cells. Compared with the SA treatment, the AN treatment increased root growth, protein content, the concentrations of most intermediates and the activity of enzymes from the TCA cycle. Furthermore, AN treatment decreased the rate of H + efflux, retarded medium acidification, decreased protein carbonylation and lipid peroxidation in roots and relieved ultrastructure disruption and increased the viability of root cells. Taken together, these results indicate that NO 3 – -dependent alleviation of NH 4 + toxicity in wheat seedlings is closely associated with physiological processes that mediate TCA cycle, relieve rhizospheric acidification and decrease the production of ROS and oxidative damage.

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“…According to our previous study, the 3-year P. notoginseng seedlings can grow normally in 5 mM ammonium solution, but were inhibited in 15 mM ammonium ( Ou et al, 2019 ). Thus, the concentration of N in the hydroponic was adopted 5 mM.…”

Section: Methodsmentioning

confidence: 99%

Lowering Nitrogen and Increasing Potassium Application Level Can Improve the Yield and Quality of Panax notoginseng

Cui

Zhu

et al. 2020

Front. Plant Sci.

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Excessive nitrogen (N) application and potassium (K) supplement deficiency is a common problem in Panax notoginseng cultivation. However, synergistic effects of lowering N and increasing K application on yield and quality of P. notoginseng have not been reported. Field experiments in two locations with different N and K combined application were conducted to study the effects on yield and quality. Then, the saponin accumulation mechanisms were explored by pot and hydroponic culture with 2- or 3-year-old seedlings. The investigation showed that 70% of P. notoginseng cultivation fields reached abundant levels of total nitrogen (TN) but had deficient levels of total potassium (TK), which may be detrimental to balance the N/K uptake of P. notoginseng. Moreover, the average biomass was 18.9 g, and P. notoginseng saponin (PNS) content was 6.95%; both were influenced by the N/K values of P. notoginseng. The field experiments indicated that compared to the conventional N and K application (N:K = 2:1), lowering N and increasing K application (N:K = 1:2) decreased root rot rate by 36.4–46.1% and increased survival rate, root biomass, and yield, as well as PNS content by 17.9–18.3, 5.7–32.9, 27.8–57.1, and 5–10%, respectively. The mechanism of lowering N and increasing K application on the PNS content improving was due to the decreasing of N/K value, which promoted photosynthesis, sugar accumulation, and the expression of saponin biosynthesis genes. Therefore, lowering N and increasing K application to the ratio of 1:2 would have great potential to improve the synergistic effect on yield and quality of P. notoginseng cultivation.

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The transcriptome variations of Panaxnotoginseng roots treated with different forms of nitrogen fertilizers

Cited by 18 publications

References 60 publications

Fertilization Regulates Accumulation and Allocation of Biomass and Nutrients in Phoebe bournei Seedlings

Fertilization Regulates Accumulation and Allocation of Biomass and Nutrients in Phoebe bournei Seedlings

Nitrate alleviates ammonium toxicity in wheat (Triticum aestivumL.) by regulating tricarboxylic acid cycle and reducing rhizospheric acidification and oxidative damage

Lowering Nitrogen and Increasing Potassium Application Level Can Improve the Yield and Quality of Panax notoginseng

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