The transferrin isoforms in chronic hepatitis

Gruszewska, Ewa; Wrona, Alicja; Gudowska, Monika; Panasiuk, Anatol; Cylwik, Bogdan; Lipartowska-Klimuk, Karina; Flisiak, Robert; Chrostek, Lech

doi:10.1016/j.clinbiochem.2017.06.013

Cited by 6 publications

(8 citation statements)

References 14 publications

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“…As expected, the total transferrin concentration was diminished in both forms of pancreatitis, with an apparent greater decrease in acute pancreatitis. Looking at our earlier works, we can only see a similarity to the profile of transferrin isoforms in chronic hepatitis [ 13 ]. At the same time, we find differences in the transferrin isoform profile in other liver diseases [ 9 ]; in rheumatic diseases, including rheumatoid arthritis, systemic lupus erythematosus, systemic sclerosis and juvenile idiopathic arthritis [ 11 , 12 , 14 ]; and in pancreatic cancers [ 6 ].…”

Section: Discussionmentioning

confidence: 94%

“…In this paper, we tried to determine the profile of transferrin isoforms in pancreatitis, in acute as well as in chronic forms of disease, in accordance with the knowledge on the changes of protein glycosylation in the course of inflammatory diseases. Because we previously assessed the profile of transferrin isoforms in many different diseases, including liver diseases, rheumatic diseases and pancreatic cancers, we have the opportunity to compare the changes in pancreatitis with the changes in the diseases mentioned above [ 6 , 10 , 11 , 13 ]. This situation created the possibility to show whether these changes are characteristic for pancreatitis or only organ-dependent inflammation.…”

Section: Discussionmentioning

confidence: 99%

“…At the same time, we find differences in the transferrin isoform profile in other liver diseases [ 9 ]; in rheumatic diseases, including rheumatoid arthritis, systemic lupus erythematosus, systemic sclerosis and juvenile idiopathic arthritis [ 11 , 12 , 14 ]; and in pancreatic cancers [ 6 ]. Thus, we can conclude that the change in transferrin isoform profile in pancreatitis is only similar to that seen in hepatitis [ 13 ]. It is also different from that observed in pancreatic cancers [ 6 ].…”

Section: Discussionmentioning

confidence: 99%

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The Serum Profile of Transferrin Isoforms in Pancreatitis

Mucha

Zaczek

Kralisz

et al. 2022

JCM

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Total transferrin concentration changes in acute-phase reactions. Additionally, the alteration of transferrin glycosylation in inflammations can occur. The aim of this study is to evaluate the effect of pancreatitis on the serum profile of transferrin isoforms. The tested groups consisted of 84 patients with acute pancreatitis and 42 patients with chronic hepatitis. Transferrin isoforms were analyzed by capillary electrophoresis on a MINICAP electrophoretic system (Sebia, France). There was a significant decrease in the concentration of pentasialotransferrin in both acute and chronic pancreatitis, and a significant increase in tetrasialotransferrin in the acute pancreatitis group when compared to the control group. There were no significant changes in transferrin isoforms between the acute and chronic pancreatitis groups, and between the edematous and necrotizing forms of the disease. Considering the etiology of acute pancreatitis, we noticed higher values of bile acids and γ-glutamyltransferase in acute pancreatitis of alcoholic etiology than that in pancreatitis of other etiologies. In conclusion, the alterations in transferrin isoform profile in acute and chronic pancreatitis are not organ specific. Because similar changes were observed in hepatitis, we can conclude that the serum profile of transferrin isoforms is involved in the pathogenesis of the disease.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The Serum Profile of Transferrin Isoforms in Pancreatitis

Mucha

Zaczek

Kralisz

et al. 2022

JCM

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“…The relative concentration of tetra- and pentasialotransferrin did not change. In our previous studies, we showed that the shift in one isoform of transferrin causes the reverse shift in the other, e.g., the increased level of tetrasialotransferrin was accompanied by the decreased level of pentasialotransferrin in the course of chronic hepatitis [ 25 ], and in pancreatic cancer [ 26 ], or mixed effect in the form of a significant decrease in tri- and pentasialotransferrin and a significant increase in tetrasialotransferrin in patients with rheumatoid arthritis [ 27 ]. Opposite changes in transferrin isoforms aim to maintain a stable level of transferrin.…”

Section: Discussionmentioning

confidence: 99%

Changed Profile of Serum Transferrin Isoforms in Primary Biliary Cholangitis

Grytczuk

Bauer

Gruszewska

et al. 2020

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Liver damage affects the synthesis of proteins and glycoproteins, and alters their posttranslational modification, such as glycosylation changing the serum profile of glycoprotein isoforms. The retention of hydrophobic bile acids in the course of cholestatic liver diseases is a major cause of liver damage in primary biliary cholangitis (PBC). The study objective was to determine the serum profile of transferrin isoforms in primary biliary cholangitis and compare it to transferrin isoforms profile in extrahepatic cholestasis. The study was carried out in 76 patients with PBC and 40 healthy blood donors. Transferrin isoforms were analyzed by the capillary electrophoresis method. The mean relative concentrations of disialotransferrin and trisialotransferrin in PBC were significantly lower than those in the healthy subjects (p < 0.001, p = 0.011; respectively). None of the transferrin isoforms changed according to the disease severity evaluated by the Ludwig scoring system. However, the disease stage affected the activity of alkaline phosphatase (ALP) and γ-glutamyl transferase (GGT), and albumin level (p = 0.002; p = 0.013 and p = 0.005, respectively). Our results indicate that serum profile of transferrin isoforms alters primary biliary cholangitis and differs in comparison to transferrin isoforms profile in extrahepatic cholestasis. The decreased concentrations of lower sialylated isoforms of transferrin (low percentage share in total transferrin level) are not associated with the histological stage of disease.

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“…Transferrin is the iron-binding serum glycoprotein which is produced in hepatocytes and contains two N-linked glycosylation sites (4,5). An abnormal glycosylation is also a known phenomenon in liver disease patients; it has been described in alcoholic liver disease (decreased enzyme activities of mannosyltransferase and galactosyltransferase, lowered intracellular dolichol concentration, desialylation of serum Tf, α1-antitrypsin and ceruloplasmin; hyperfucosylation of haptoglobin and serum Tf), galactosemia and fructosemia (the pattern resembling CDG type I), chronic hepatitis B and C, non-alcoholic steatohepatitis, and bile-related liver diseases (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21). Despite of IEF, there are also some other methods, like HPLC or MALDI-TOF but they are not available in Poland.…”

Section: Introductionmentioning

confidence: 99%

Pediatric Liver Disease Patients and Secondary Glycosylation Abnormalities

et al. 2021

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Background: Isoelectric focusing (IEF) of serum transferrin (Tf) is still the method of choice for diagnosis of congenital disorders of glycosylation (CDG). An abnormal glycosylation is also a known phenomenon in adult liver disease patients. The aim of this study was to characterize glycosylation disturbances in pediatric patients with primary liver disease. However, there are no reports of this phenomenon in children.Materials and Methods: Between 1995 and 2019, circa 2,000 serum Tf isoform analyses have been performed in children with primary liver diseases; some of them underwent subsequent analyses. We enrolled in this study 19 patients who developed an acute liver injury (ALI)/failure (ALF) or exhibited a chronic liver disease (CLD) and were evaluated and listed for liver transplantation (LTx) or had just undergone this procedure, and secondary abnormal serum Tf isoform profile.Results: Among 12 patients with ALI/ALF, 10 had an increased percentage of asialo-, monosialo-, and disialo-Tf isoforms. All patients with CLD had an increased percentage of asialo- and monosialo-Tf isoform. Two patients diagnosed with recurrent ALF had very specific serum Tf profile with a huge increase in the asialo- and monosialo-Tf isoform. On follow-up analyses (available in some patients), serum Tf IEF profile normalized in parallel to normalization of liver function tests, spontaneously or during treatment, including glucocorticosteroids in AIH, LTx in CLD.Conclusions: All pediatric patients with primary liver disease had increased asialo-Tf as well as monosialo-Tf isoforms. None of them had elevated percentage of trisialo-Tf isoform.

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The transferrin isoforms in chronic hepatitis

Cited by 6 publications

References 14 publications

The Serum Profile of Transferrin Isoforms in Pancreatitis

The Serum Profile of Transferrin Isoforms in Pancreatitis

Changed Profile of Serum Transferrin Isoforms in Primary Biliary Cholangitis

Pediatric Liver Disease Patients and Secondary Glycosylation Abnormalities

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