The transition of the thick ascending limb of Henle's loop into the distal convoluted tubule in the nephron of the rat kidney

Kaissling, Brigitte; Peter, St.; Kriz, Wilhelm

doi:10.1007/bf00222059

Cited by 60 publications

(12 citation statements)

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“…Ultrastructural identification of renal epithelial cells was carried out according to their descriptions as set out by Tisher et al (1968), , Allen & Tisher (1976) and Kaissling et al (1977). After the careful study of several hundred renal tubules from 15 biopsies the pre-embedding and post-embedding immunolabelling techniques were found to give similar results.…”

Section: Resultsmentioning

confidence: 99%

“…These results should be questioned, since the authors do not imply a proper distinction between ascending limb and distal tubule cells. According to current knowledge, the transition from the ascending limb to the distal convoluted tubule occurs distal to the macula densa (Kaissling et al, 1977). Sikri et al termed the cells adjacent to the macula densa as being distal convoluted tubule cells when, in fact, they are ascending limb cells.…”

Section: Discussionmentioning

confidence: 98%

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Ultrastructural localization of Tamm—Horsfall protein in human kidney using immunogold electron microscopy

et al. 1988

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The ultrastructural localization of Tamm-Horsfall protein (THP) was studied in paraformaldehyde-fixed human renal biopsies. Pre-embedding and post-embedding immunogold labelling techniques were developed utilizing a monoclonal antibody specific for human urinary THP. With the pre-embedding technique, membrane contrast was enhanced by osmification thus allowing precise localization of gold particles. Reasonable tissue penetration of antibodies was achieved without compromising ultrastructural detail. The hydrophilic resin LR White was used for post-embedding labelling to ensure maximum penetration of antibodies. However, sections had only mild osmification and consequently localization of label was less certain. Both labelling techniques gave similar results. THP was found to be associated with two renal cell types. Epithelial cells lining the thick ascending limb of Henle's loop had gold label closely associated with the whole cell plasmalemma, with some of these cells having an apparently random distribution of label throughout the cytoplasm. Only the luminal plasmalemma of epithelial cells lining distal convoluted tubules were found to be labelled. Basolateral membranes and the cytoplasm of these cells were negative. The use of a monoclonal antibody of defined specificity combined with the two immunolabelling procedures represents a precise reliable method for studying ultrastructural localization of THP in the human kidney.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 98%

Ultrastructural localization of Tamm—Horsfall protein in human kidney using immunogold electron microscopy

et al. 1988

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“…The thick ascending limb of Henle (TALI in the rat kidney has received limited attention from morphologists (Suzuki and Mostofi, 1967;Allen and Tisher, 1976;Kaissling et al, 1977;Tisher, 1981), despite the fact that physiologists have reported considerable heterogeneity of function in this region of the nephron (Jacobson, 1981;Burg, 1982). In a study of the rabbit kidney, Kaissling and Kriz (1979) described a gradual decrease in cell height as the TAL ascends through the outer medulla to the cortex.…”

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confidence: 96%

Ultrastructure of the thick ascending limb of henle in the rat kidney

1984

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The thick ascending limb of Henle (TAL) in the rat until recently has been considered a morphologically homogeneous structure despite physiologic and biochemical evidence to the contrary. The present study was designed to examine the ultrastructural characteristics of the TAL in the inner cortex and the outer and inner stripes of the outer medulla using qualitative and quantitative transmission electron microscopy. Kidneys of male Sprague-Dawley rats were preserved by in vivo perfusion with glutaraldehyde for light and electron microscopy. The peritubular diameter and cell height were determined by direct measurements on tubule cross sections. Morphometric analyses were performed on montages of tubule cross sections. The peritubular diameter of the TAL was similar in the three regions under investigation, but the TAL cells were taller in the inner stripe than in the inner cortex and outer stripe. Morphometry revealed significant differences between the three regions with respect to the mean tubular cross-sectional area (AT), the surface density (SV), and the surface area per mm of tubule (ST) of apical and basolateral plasma membranes, and the volume density (VV) of mitochondria. The major morphologic division appeared to be between the inner stripe segment and the remainder of the TAL. These findings document the presence of significant morphologic heterogeneity of the rat TAL.

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“…These specialized TAL cells directly contact the extraglomerular mesangium of the corresponding glomerulus (73). The function of MD cells is dual: MD cells sense the salt concentration in the tubular fluid and influence 1) preglomerular resistance and, consequently, the snGFR and 2) the secretion of renin Review F997 PHYSIOLOGY OF NKCC2 from the juxtaglomerular granular (JG) cells of the afferent arteriole (22,137,143).…”

Section: The Regulatory Function Of Nkcc2: MD Control Of Preglomerulamentioning

confidence: 99%

Physiology and pathophysiology of the renal Na-K-2Cl cotransporter (NKCC2)

Castrop

Schießl

2014

American Journal of Physiology-Renal Physiology

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The Na-K-2Cl cotransporter (NKCC2; BSC1) is located in the apical membrane of the epithelial cells of the thick ascending limb of the loop of Henle (TAL). NKCC2 facilitates ∼20-25% of the reuptake of the total filtered NaCl load. NKCC2 is therefore one of the transport proteins with the highest overall reabsorptive capacity in the kidney. Consequently, even subtle changes in NKCC2 transport activity considerably alter the renal reabsorptive capacity for NaCl and eventually lead to perturbations of the salt and water homoeostasis. In addition to facilitating the bulk reabsorption of NaCl in the TAL, NKCC2 transport activity in the macula densa cells of the TAL constitutes the initial step of the tubular-vascular communication within the juxtaglomerular apparatus (JGA); this communications allows the TAL to modulate the preglomerular resistance of the afferent arteriole and the renin secretion from the granular cells of the JGA. This review provides an overview of our current knowledge with respect to the general functions of NKCC2, the modulation of its transport activity by different regulatory mechanisms, and new developments in the pathophysiology of NKCC2-dependent renal NaCl transport.

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The transition of the thick ascending limb of Henle's loop into the distal convoluted tubule in the nephron of the rat kidney

Cited by 60 publications

References 9 publications

Ultrastructural localization of Tamm—Horsfall protein in human kidney using immunogold electron microscopy

Ultrastructural localization of Tamm—Horsfall protein in human kidney using immunogold electron microscopy

Ultrastructure of the thick ascending limb of henle in the rat kidney

Physiology and pathophysiology of the renal Na-K-2Cl cotransporter (NKCC2)

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