The translational system from wheat embryos: Some properties of the polypeptides associated in EF1<sub>H</sub>

Lanzani, G.A.; Caldiroli, E.; Manzocchi, Lucia A.; Bollini, Roberto; Alberti, L. De

doi:10.1016/0014-5793(76)80260-8

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1976

1989

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Cited by 14 publications

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“…In a preliminary study [18], we reported on a factor able to bind the artificial mRNA poly(U), which we found in the EF-lH fraction from wheat embryos. This paper shows that most of this factor is present in the 1 0 0 0 0 0 x~ supernatant fraction which contains EF-1 and that this factor, under our experimcntal conditions, is always purified with some EF-1 L. The two factors can be separated only by a heating step, which destroys the EF-I activity.…”

Section: Discussionmentioning

confidence: 86%

A Poly(U)-Binding Factor Stimulating EF-1 Activity in the Wheat-Germ Soluble Fraction

1983

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A poly(U)‐binding activity is present in the high‐speed supernatant fraction of embryo homogenates from wheat seeds. The factor responsible for such activity was found to have a stimulatory effect on the elongation factor 1 (EF‐1). It copurifies with EF‐1L, the lighter form of EF‐1, through Sephadex G‐200, DEAE‐cellulose, hydroxyapatite and poly(U)‐Sepharose 4B column chromatography. The two factors could be separated only through a heating step which destroyed EF‐1 activity whilst leaving most of the poly(U)‐binding activity unaltered.

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Section: Discussionmentioning

confidence: 86%

A Poly(U)-Binding Factor Stimulating EF-1 Activity in the Wheat-Germ Soluble Fraction

1983

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Glycoproteins of the Synapse

Gurd

1989

Neurobiology of Glycoconjugates

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Function of eukaryotic ribosomes

Bielka¹

1982

The Eukaryotic Ribosome

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The translational system from wheat embryos: Some properties of the polypeptides associated in EF1_H

Cited by 14 publications

References 14 publications

A Poly(U)-Binding Factor Stimulating EF-1 Activity in the Wheat-Germ Soluble Fraction

A Poly(U)-Binding Factor Stimulating EF-1 Activity in the Wheat-Germ Soluble Fraction

Glycoproteins of the Synapse

Function of eukaryotic ribosomes

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The translational system from wheat embryos: Some properties of the polypeptides associated in EF1H

Cited by 14 publications

References 14 publications

A Poly(U)-Binding Factor Stimulating EF-1 Activity in the Wheat-Germ Soluble Fraction

A Poly(U)-Binding Factor Stimulating EF-1 Activity in the Wheat-Germ Soluble Fraction

Glycoproteins of the Synapse

Function of eukaryotic ribosomes

Contact Info

Product

Resources

About

The translational system from wheat embryos: Some properties of the polypeptides associated in EF1_H