The transmission of <i>Actinomyces rhodnii</i> Erikson in <i>Rhodnius prolixus</i> stål (hemiptera) and its influence on the growth of the host

Brecher, G.; Wigglesworth, V. B.

doi:10.1017/s0031182000021648

Cited by 106 publications

(68 citation statements)

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“…Extracellular symbionts of hemipterans are horizontally transmitted by a multitude of routes, with coprophagy, cannibalism and environmental determination being the most prominent transmission strategies (see [34] for a review). The first studies on triatomine gut microbiota transmission hypothesized a horizontal route for the symbiont Rhodoccocus in R. prolixus via coprophagy of dry excreta [35]. …”

Section: Discussionmentioning

confidence: 99%

Triatomine bugs, their microbiota and Trypanosoma cruzi: asymmetric responses of bacteria to an infected blood meal

et al. 2016

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BackgroundTriatomine bugs (Hemiptera: Reduviidae) are vectors of the flagellate Trypanosoma cruzi, the causative agent of Chagas disease. The study of triatomine gut microbiota has gained relevance in the last years due to its possible role in vector competence and prospective use in control strategies. The objective of this study is to examine changes in the gut microbiota composition of triatomines in response to a T. cruzi-infected blood meal and identifying key factors determining those changes.ResultsWe sampled colony-reared individuals from six triatomine vectors (Panstrongylus megistus, Rhodnius prolixus, Triatoma brasiliensis, T. infestans, T. juazeirensis and T. sherlocki) comparing experimentally T. cruzi strain 0354-challenged and non-challenged insects. The microbiota of gut and gonad tissues was characterized using high throughput sequencing of region V3-V4 of bacterial 16S rRNA gene. The triatomine microbiota had a low intra-individual diversity, and a high inter-individual variation within the same host species. Arsenophonous appeared as the dominant triatomine bacterial symbiont in our study (59% of the total 16S coverage), but there were significant differences in the distribution of bacterial genera among vectors. In Rhodnius prolixus the dominant symbiont was Pectobacterium.Conclusions Trypanosoma cruzi-challenge significantly affects microbiota composition, with challenged vectors harbouring a significantly more diverse bacterial community, both in the gut and the gonads. Our results show that blood-feeding with T. cruzi epimastigotes strongly affects microbiota composition in a species-specific manner. We suggest that triatomine-adapted enterobacteria such as Arsenophonus could be used as stable vectors for genetic transformation of triatomine bugs and control of Chagas disease.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1926-2) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Triatomine bugs, their microbiota and Trypanosoma cruzi: asymmetric responses of bacteria to an infected blood meal

et al. 2016

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“…In addition, it has been shown that T. rangeli can reduce symbiont populations [26], [28] producing several negative effects on insect development [20].…”

Section: Introductionmentioning

confidence: 99%

Effects of Infection by Trypanosoma cruzi and Trypanosoma rangeli on the Reproductive Performance of the Vector Rhodnius prolixus

et al. 2014

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The insect Rhodnius prolixus is responsible for the transmission of Trypanosoma cruzi, which is the etiological agent of Chagas disease in areas of Central and South America. Besides this, it can be infected by other trypanosomes such as Trypanosoma rangeli. The effects of these parasites on vectors are poorly understood and are often controversial so here we focussed on possible negative effects of these parasites on the reproductive performance of R. prolixus, specifically comparing infected and uninfected couples. While T. cruzi infection did not delay pre-oviposition time of infected couples at either temperature tested (25 and 30°C) it did, at 25°C, increase the e-value in the second reproductive cycle, as well as hatching rates. Meanwhile, at 30°C, T. cruzi infection decreased the e-value of insects during the first cycle and also the fertility of older insects. When couples were instead infected with T. rangeli, pre-oviposition time was delayed, while reductions in the e-value and hatching rate were observed in the second and third cycles. We conclude that both T. cruzi and T. rangeli can impair reproductive performance of R. prolixus, although for T. cruzi, this is dependent on rearing temperature and insect age. We discuss these reproductive costs in terms of potential consequences on triatomine behavior and survival.

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“…These bacteria -mainly actinomycetes -include species of Rhodococcus, Nocardia and Gordonia (Brecher & Wigglesworth 1944). An experimental infection of the vector R. prolixus with T. rangeli, but not with T. cruzi or Blastocrithidia triatomae, reduced the Fig.…”

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confidence: 99%

Developmental and reproductive patterns of Triatoma brasiliensis infected with Trypanosoma cruzi under laboratory conditions

Oliveira¹,

Carvalho-Costa

Gomes³

et al. 2010

Mem. Inst. Oswaldo Cruz

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The transmission of Actinomyces rhodnii Erikson in Rhodnius prolixus stål (hemiptera) and its influence on the growth of the host

Cited by 106 publications

References 10 publications

Triatomine bugs, their microbiota and Trypanosoma cruzi: asymmetric responses of bacteria to an infected blood meal

Triatomine bugs, their microbiota and Trypanosoma cruzi: asymmetric responses of bacteria to an infected blood meal

Effects of Infection by Trypanosoma cruzi and Trypanosoma rangeli on the Reproductive Performance of the Vector Rhodnius prolixus

Developmental and reproductive patterns of Triatoma brasiliensis infected with Trypanosoma cruzi under laboratory conditions

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