The Transport of Steroid Hormones into Animal Cells

Giorgi, Eleonora P.

doi:10.1016/s0074-7696(08)61959-2

Cited by 51 publications

(18 citation statements)

References 123 publications

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“…The results obtained for the 17~,20fl-DHP uptake into the oocytes are in agreement with the generally accepted concept of steroid transport into animal cells occurring by passive diffusion (Giorgi 1980). Since it is possible that the physiological action of N'IIS occurs through its interaction with oocyte plasma naembrarm receptors (Maneckjee el a/.…”

Section: Discussionsupporting

confidence: 86%

Transport of 17α,20β-dihydroxy-4-pregnen-3-one in brook troutSalvelinus fontinalis ovarian follicles

Maneckjee

Idler

Weisbart

1991

Fish Physiol Biochem

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Extremely low levels of the maturation inducing steroid (MIS) 17α,20β-dihydroxy-4-pregnen-3-one (17α,20β-DHP) were found in the ooplasm and ovarian follicle membranes of Atlantic salmonSalmo salar ouananiche, a finding that is at variance with the elevated blood levels of the steroid. The uptake of MIS at physiological concentrations into brook trout follicles occurred by passive diffusion. Uptake of the steroid into the ovarian follicle membrane, consisting of zona radiata and the attached follicle cells, deviated from linearity in a double reciprocal plot. These results suggest that 17α,20β-DHP is binding to a receptor-like protein in the ovarian follicle or the zona radiata membrane surrounding the oocyte, and extend our previous demonstration of 17α,20β-DHP receptor-like activity in the zona radiata membrane of the late stage brook trout oocytes. An oocyte cytoplasmic receptor gave subunits on SDS PAGE that were similar to the membrane and cytosol receptors previously described.

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Section: Discussionsupporting

confidence: 86%

Transport of 17α,20β-dihydroxy-4-pregnen-3-one in brook troutSalvelinus fontinalis ovarian follicles

Maneckjee

Idler

Weisbart

1991

Fish Physiol Biochem

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“…It is usually believed that steroid hormones penetrate into the cell by passive diffusion (Giorgi, 1980;Lovell-Smith & Garcfa-Webb, 1986). This model is based on the lipophilic nature of steroid molecules and on their affinity for lipid constituents of the plasma membranes (Giorgi & Stein, 1981).…”

Section: Introductionmentioning

confidence: 99%

Evidence for the presence of specific binding sites for corticoids in mouse liver plasma membranes

et al. 1989

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The specific binding of [3H]cortisol to plasma membranes purified from mouse liver, studied by the ultrafiltration method, shows the existence of specific binding sites for cortisol. The kinetic parameters of this binding are KD = 4.4 nM and Bmax = 685 fmol/mg protein in presence of 1 microM of corticosterone. With respect to the binding of 4 nM [3H]cortisol to the membrane, the affinities of the steroids decreased in the following order: deoxycorticosterone greater than corticosterone greater than progesterone greater than cortisol greater than prednisolone greater than testosterone greater than 20 beta-hydroxyprogesterone greater than cortisone. Estradiol, dexamethasone, ouabain and triamcinolone acetonide do not have affinity for this binding site. Neither Ca2+ nor Mg2+ affected the binding of [3H]cortisol to the plasma membranes. Likewise, the presence of agonists and antagonists of alpha and beta-adrenergic receptors did not modify the binding of [3H]cortisol. The results suggest that the plasma membrane binding site characterized is more specific for corticoids and is different from nuclear glucocorticoid and progesterone receptors.

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“…Information about the mechanisms by which steroids are transported across epithelia is limited (see Giorgi, 1980). More than 90% of progesterone in milk is associated with the lipid fraction, and milk pro¬ gesterone concentrations are closely correlated with milk fat content (Heap, Henville & Linzell, 1975).…”

mentioning

confidence: 99%

Transport of oestrone sulphate by the mammary gland in the goat

Heap¹,

Hamon²,

Fleet³

1984

Journal of Endocrinology

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During pregnancy in goats the concentration of endogenous oestrone sulphate in milk increased more than twofold, and that in arterial and mammary venous plasma 10- and 20-fold respectively. The concentration in milk was higher than that in arterial plasma, particularly in lactating goats during mid-gestation. This was partly related to mammary production of oestrone sulphate (or of a closely related steroid which cross-reacted in the radioimmunoassay) since in tracer infusion studies the specific activity of oestrone sulphate in milk was significantly lower than that in arterial or mammary venous plasma. It was also related to the existence of a mechanism within the gland which concentrates oestrone sulphate in milk since when infused close-arterially into the mammary gland of a non-pregnant goat with undetectable levels of the endogenous compound in the circulation, a concentration ratio of 7.4:1.0 was reached for oestrone sulphate in milk:arterial plasma. Tracer kinetic studies showed that mammary extraction of [3H]oestrone sulphate was variable (up to 41.3 +/- 30.6%, mean +/- S.E.M.). During intravenous or close-arterial infusion, radioactivity in arterial and mammary venous plasma at steady state was mainly in the form of [3H]oestrone sulphate (range, 64 +/- 10.6 to 80.2 +/- 5.9% of total radioactivity in plasma). The remainder was in the form of compounds chromatographically similar to oestradiol-17 beta-3-monosulphate, oestradiol-17 alpha-3-monosulphate and unconjugated oestrogens. The distribution of radioactivity between these different steriods was similar in arterial mammary venous plasma indicating a low level of selective mammary metabolism or extraction. The amount of labelled oestrone sulphate transferred into milk was low, and it was significantly less in pregnant (range, 0.11 +/- 0.07 to 0.27 +/- 0.16% of total infusate) than in non-pregnant animals (3.23 +/- 0.50%). Studies of the rate of transfer of [3H]oestrone sulphate from blood to milk indicated the presence of a transcellular route with peak activity in milk occurring about 110 min after the start of the infusion.

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The Transport of Steroid Hormones into Animal Cells

Cited by 51 publications

References 123 publications

Transport of 17α,20β-dihydroxy-4-pregnen-3-one in brook troutSalvelinus fontinalis ovarian follicles

Transport of 17α,20β-dihydroxy-4-pregnen-3-one in brook troutSalvelinus fontinalis ovarian follicles

Evidence for the presence of specific binding sites for corticoids in mouse liver plasma membranes

Transport of oestrone sulphate by the mammary gland in the goat

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