The tRNA-Derived Fragment-3017A Promotes Metastasis by Inhibiting NELL2 in Human Gastric Cancer

Tong, Linhao; Zhang, Weixu; Qu, Bicheng; Zhang, Fei; Wu, Zhonghua; Shi, Jinxin; Chen, Xiaowan; Song, Yongxi; Wang, Zhenning

doi:10.3389/fonc.2020.570916

Cited by 49 publications

(39 citation statements)

References 60 publications

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“…demonstrated that tRF-3019a enhanced cell proliferation, migration, and invasion by targeting FBXO47, and it might serve as a potential diagnostic biomarker for GC ( 36 ). Similar results were found that tRF-3017A might play an important role in promoting migration and invasion by silencing NELL2 in GC ( 37 ). In this study, tRFs were screened from GC tissues by high-throughput sequencing.…”

Section: Discussionsupporting

confidence: 86%

“…tRF-3017A was highly expressed in tissues and cell lines of GC and was positively correlated with lymph node metastasis. It may be that tRF-3017A promotes the migration and invasion of GC cells by silencing the tumor suppressor NELL2 ( 37 ). In the previous study, our team also found that serum hsa_tsr016141 has good stability and specificity and could be used for dynamic monitoring of patients with GC ( 38 ).…”

Section: Introductionmentioning

confidence: 99%

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Elucidating the Role of Serum tRF-31-U5YKFN8DYDZDD as a Novel Diagnostic Biomarker in Gastric Cancer (GC)

Huang

Zhang

et al. 2021

Front. Oncol.

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BackgroundGastric cancer (GC) is one of the malignant tumors with the highest morbidity and mortality in the world. Early diagnosis combined with surgical treatment can significantly improve the prognosis of patients. Therefore, it is urgent to seek higher sensitivity and specificity biomarkers in GC. tRNA-derived small RNAs are a new non-coding small RNA that widely exists in tumor cells and body fluids. In this study, we explore the expression and biological significance of tRNA-derived small RNAs in GC.Materials and MethodsFirst of all, we screened the differentially expressed tRNA-derived small RNAs in tumor tissues by high-throughput sequencing. Agarose gel electrophoresis (AGE), Sanger sequencing, and Nuclear and Cytoplasmic RNA Separation Assay were used to screen tRF-31-U5YKFN8DYDZDD as a potential tumor biomarker for the diagnosis of GC. Then, we detected the different expressions of tRF-31-U5YKFN8DYDZDD in 24 pairs of GC and paracancerous tissues, the serum of 111 GC patients at first diagnosis, 89 normal subjects, 48 superficial gastritis patients, and 28 postoperative GC patients by quantitative real-time PCR (qRT-PCR). Finally, we used the receiver operating characteristic (ROC) curve to analyze its diagnostic efficacy.ResultsThe expression of tRF-31-U5YKFN8DYDZDD has good stability and easy detection. tRF-31-U5YKFN8DYDZDD was highly expressed in tumor tissue, serum, and cell lines of GC, and the expression was significantly related to TNM stage, depth of tumor invasion, lymph node metastasis, and vascular invasion. The expression of serum tRF-31-U5YKFN8DYDZDD in the GC patients decreased after the operation (P = 0.0003). Combined with ROC curve analysis, tRF-31-U5YKFN8DYDZDD has better detection efficiency than conventional markers.ConclusionsThe expressions of tRF-31-U5YKFN8DYDZDD in the tumor and paracancerous tissues, the serum of GC patients and healthy people, and the serum of GC patients before and after operation were different. tRF-31-U5YKFN8DYDZDD is not only a diagnostic biomarker of GC but also a predictor of poor prognosis.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Elucidating the Role of Serum tRF-31-U5YKFN8DYDZDD as a Novel Diagnostic Biomarker in Gastric Cancer (GC)

Huang

Zhang

et al. 2021

Front. Oncol.

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“…84 A direct correlation of tRNA fragments with metastasis and cell proliferation has been shown recently. Tong et al 45 have reported that tRNA-Val TAC -derived tRF-3017A promotes metastasis in gastric cancer by regulating the NELL2 tumor suppressor gene. 45 The tumor suppressor transcription factor (TF) RUNX1 plays a significant role in initiation and metastasis of breast cancer.…”

Section: Tsrnas and Cancermentioning

confidence: 99%

Regulatory roles of tRNA-derived RNA fragments in human pathophysiology

Pandey

Madhry

Kumar³

et al. 2021

Molecular Therapy - Nucleic Acids

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Hundreds of tRNA genes and pseudogenes are encoded by the human genome. tRNAs are the second most abundant type of RNA in the cell. Advancement in deep-sequencing technologies have revealed the presence of abundant expression of functional tRNA-derived RNA fragments (tRFs). They are either generated from precursor (pre-)tRNA or mature tRNA. They have been found to play crucial regulatory roles during different pathological conditions. Herein, we briefly summarize the discovery and recent advances in deciphering the regulatory role played by tRFs in the pathophysiology of different human diseases.

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“…Meanwhile, the metastasis regulatory functions of tsRNAs are also found in other malignancies. tRF-3017A was found to silence the tumor suppressor NELL2 through an AGO-dependent mechanism, thereby promoting the invasion and migration of gastric cancer cells [ 69 ]. tRF-17 was found to act as an invasion inhibitor by regulating the THBS1/TGF-β1/smad3 axis in breast cancer cells [ 70 ].…”

Section: Tsrnas and Cancer Hallmarksmentioning

confidence: 99%

tRNA-derived small RNAs: novel regulators of cancer hallmarks and targets of clinical application

Liu

Zhao

et al. 2021

Cell Death Discov.

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AbstracttRNAs are a group of conventional noncoding RNAs (ncRNAs) with critical roles in the biological synthesis of proteins. Recently, tRNA-derived small RNAs (tsRNAs) were found to have important biological functions in the development of human diseases including carcinomas, rather than just being considered pure degradation material. tsRNAs not only are abnormally expressed in the cancer tissues and serum of cancer patients, but also have been suggested to regulate various vital cancer hallmarks. On the other hand, the application of tsRNAs as biomarkers and therapeutic targets is promising. In this review, we focused on the basic characteristics of tsRNAs, and their biological functions known thus far, and explored the regulatory roles of tsRNAs in cancer hallmarks including proliferation, apoptosis, metastasis, tumor microenvironment, drug resistance, cancer stem cell phenotype, and cancer cell metabolism. In addition, we also discussed the research progress on the application of tsRNAs as tumor biomarkers and therapeutic targets.

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The tRNA-Derived Fragment-3017A Promotes Metastasis by Inhibiting NELL2 in Human Gastric Cancer

Cited by 49 publications

References 60 publications

Elucidating the Role of Serum tRF-31-U5YKFN8DYDZDD as a Novel Diagnostic Biomarker in Gastric Cancer (GC)

Elucidating the Role of Serum tRF-31-U5YKFN8DYDZDD as a Novel Diagnostic Biomarker in Gastric Cancer (GC)

Regulatory roles of tRNA-derived RNA fragments in human pathophysiology

tRNA-derived small RNAs: novel regulators of cancer hallmarks and targets of clinical application

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