2012
DOI: 10.1074/jbc.m112.390112
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The tRNA Recognition Mechanism of Folate/FAD-dependent tRNA Methyltransferase (TrmFO)

Abstract: Background: RNA modification enzymes select specific RNAs as substrates. Results: A novel assay for folate-dependent tRNA methyltransferase (TrmFO) was developed that clarified positive and negative determinants of TrmFO. Conclusion: TrmFO recognizes a T-arm structure including the U54U55C56 sequence and G53-C61 base pair; A38 prevents incorrect methylation of U32. Significance: Studying how proteins recognize RNA is crucial for understanding RNA maturation processes.

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Cited by 38 publications
(29 citation statements)
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“…; Yamagami et al . ), supports the effect of Ψ55 on maintenance of tRNA flexibility through the network at lower temperatures (Yamagami et al . ).…”
Section: Introductionsupporting
confidence: 69%
See 1 more Smart Citation
“…; Yamagami et al . ), supports the effect of Ψ55 on maintenance of tRNA flexibility through the network at lower temperatures (Yamagami et al . ).…”
Section: Introductionsupporting
confidence: 69%
“…The pseudouridine (Ψ) at position 55, which is formed by TruB (Nurse et al 1995), is required for survival of T. thermophilus at relatively low temperatures (50°C) (Ishida et al 2011). The 5-methyluridine (m 5 U) at position 54, which is produced by TrmFO (Urbonavicius et al 2005;Yamagami et al 2012), supports the effect of Ψ55 on maintenance of tRNA flexibility through the network at lower temperatures (Yamagami et al 2016). In contrast, for the survival of T. thermophilus at high temperatures (80°C), three modified nucleosides, m 5 s 2 U54 (Shigi et al 2006), m 1 A58 ) and N 7 -methylguanosine (m 7 G) at position 46 (Tomikawa et al 2010) in tRNA, are essential.…”
Section: Introductionmentioning
confidence: 99%
“…In brief, T. thermophilus can grow at a wide range of temperatures (50−83 °C). To facilitate this, a network in T. thermophilus regulates the modification levels of Gm18, 5-methyl-2-thiouridine at position 54 (m 5 s 2 U54) [102,103,104,105,106] and m 1 A58 [107,108] in tRNA, controls the flexibility (rigidity) of tRNA, and contributes to efficient protein synthesis at various temperatures [109,110,111]. Although the Gm18 modification itself increases the melting temperature of tRNA Phe by only 0.5 °C, the combination of these three modified nucleosides increases the melting temperature by nearly 10 °C [110].…”
Section: Functions Of Modified Nucleosides Synthesized By Spout Trmentioning
confidence: 99%
“…Thr Accelerate the Methylation by TrmI-Recently, we reported that the tRNA modification enzymes and modified nucleotides in T. thermophilus form a network, which regulates the extent of modifications in tRNA in response to changes in temperature (2,34,35,51,55 U54 modification increases the melting temperature of the tRNA by more than 4°C (32,33). Furthermore, the combination of the Gm18, m 5 s 2 U54, and m 1 A58 modifications increases the melting temperature by nearly 10°C as compared with the unmodified transcript (35).…”
Section: The Other Modifications In Trna Ggumentioning
confidence: 99%