The trophocytes and oenocytes of worker and queen honey bees (Apis mellifera) exhibit distinct age-associated transcriptome profiles

Lu, Cheng-Yen; Weng, Yu‐Ting; Tan, Bertrand Chin‐Ming; Hsu, Chin-Yuan

doi:10.1007/s11357-021-00360-y

Cited by 3 publications

(2 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The RNAi effect of ferritin2 ingestion is similar to the one from the injection RNAi effect that inhibits the mRNA expression and protein synthesis of ferritin2 [ 14 ]; furthermore, ferritin2 dsRNA ingestion has a long-term knockdown effect. The RNAi effect of ferritin2 daily ingestion corresponds with previous studies showing that the feeding of Israeli acute paralysis virus (IAPV)-dsRNA lowers IAPV level and prevents bee’s mortality [ 25 ] and the feeding of DWV-dsRNA reduces wing deformity [ 28 ].…”

Section: Discussionsupporting

confidence: 77%

See 1 more Smart Citation

Long-term inhibition of ferritin2 synthesis in trophocytes and oenocytes by ferritin2 double-stranded RNA ingestion to investigate the mechanisms of magnetoreception in honey bees (Apis mellifera)

Hsu

Weng

2021

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Behavioral studies indicate that honey bees (Apis mellifera) have a capacity for magnetoreception and superparamagnetic magnetite is suggested to be a magnetoreceptor. The long-term inhibition of magnetite formation can be employed to explore the bee’s magnetoreception. A recent study shows that magnetite formation, ferritin2 messenger RNA (mRNA) expression, and the protein synthesis of ferritin2 in trophocytes and oenocytes were all inhibited by a single injection of ferritin2 double-stranded RNA (dsRNA) into the hemolymph of honey bees but how to maintain this knockdown of ferritin2 for the long-term is unknown. In this study, we injected ferritin2 dsRNA into the hemolymph of worker bees three times every six days to maintain long-term inhibition; however, multi-microinjections accelerated the death of the bees. To overcome this problem, we further reared newly emerged worker bees daily with ferritin2 dsRNA throughout their lives, demonstrating no impact on their lifespans. Follow-up assays showed that the mRNA expression and protein synthesis of ferritin2 were persistently inhibited. These findings verified that daily ferritin2 dsRNA ingestion not only displays the long-term inhibition of mRNA expression and protein synthesis of ferritin2, but also did not damage the bees. This method of long-term inhibition can be used in behavioral studies of magnetoreception in honey bees.

show abstract

Section: Discussionsupporting

confidence: 77%

“…Primer sequences were noted above. The β-actin gene was used as a reference gene [ 25 ]. The primers were designed according to the nucleotide sequences available in GenBank: β-actin (AB023025): forward , reverse .…”

Section: Methodsmentioning

confidence: 99%