The Tumor Cells (FA-6) Established from a Pancreatic Cancer Associated with Humoral Hypercalcemia of Malignancy: A Simultaneous Production of Parathyroid Hormone-Like Activity and Transforming Growth Factor Activity.

Nagata, Noriko; Akatsu, Takuhiko; Kugai, Nobuo; Yasutomo, Yoshiro; Kinoshita, Tokuyasu; Kosano, Hiroshi; Shimauchi, Takehide; Takatani, Osamu; Ueyama, Yoshito

doi:10.1507/endocrj1954.36.75

Cited by 24 publications

(13 citation statements)

References 29 publications

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“…Nineteen pancreatic cancer cell lines were analyzed in this study: A818-4, 4 AsPC-1, 5 Capan-2, 6 CFPAC-1, 7 Colo-357, 8 FA6, 9 HPAF-II, 10 Hs766T, 11 IMIM-PC-2, 12 MDAPanc-3, 13 MiaPaCa-2, 14 PaCa-3, 15 Panc-1, 16 PaTu-I, PaTu-II, 17 PT45, 18 RWP, 19 SUIT-2 20 and T3M4. 21 These cell lines were obtained from Cancer Research U.K. cell production services (London Research Institute Clare Hall Laboratories, Potters Bar, U.K.) and cultured in E4 medium supplemented with 10% heat-inactivated fetal calf serum (Gibco-Life Technologies, Paisley, U.K.) until harvest when they reached 80% confluence.…”

Section: Cell Culture and Rna Isolationmentioning

confidence: 99%

Analysis of gene expression in cancer cell lines identifies candidate markers for pancreatic tumorigenesis and metastasis

Missiaglia

Blaveri

Terris

et al. 2004

Intl Journal of Cancer

139

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Pancreatic cancer is a highly aggressive type of malignancy and the prognosis for disease presenting typically at a late stage is extremely poor. A comprehensive understanding of its molecular genetics is required in order to develop new approaches to clinical management. To date, serial analysis of gene expression and more recently oligo/cDNA microarray technologies have been employed in order to identify genes involved in pancreatic neoplasia that can be developed as diagnostic markers and drug targets for this dismal disease. This study describes the expression profile obtained from 20 pancreatic cell lines using cDNA microarrays containing 9,932 human gene elements. Numerous genes were identified as being differentially expressed, some of which have been previously implicated in pancreatic adenocarcinoma (S100P, S100A4, prostate stem cell antigen, lipocalin 2, claudins 3 and 4, trefoil factors 1 and 2) as well as several novel genes. The differentially expressed genes identified are involved in a variety of cellular functions, including control of transcription, regulation of the cell cycle, proteolysis, cell adhesion and signaling. Validation of our array results was performed by exploring the SAGEmap database and by immunohistochemistry for a selection of 4 genes that have not previously been studied in pancreatic cancer: anterior gradient 2 homologue (Xenopus laevis), insulin-like growth factor binding protein 3 and 4 and Forkhead box J1. Immunostaining was performed using pancreas-specific tissue microarrays containing core biopsies from 305 clinical specimens. In addition, using statistical group comparison and hierarchical clustering, a selection of genes was identified that may be linked to the site of metastasis from which these cell lines were isolated.

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Section: Cell Culture and Rna Isolationmentioning

confidence: 99%

Analysis of gene expression in cancer cell lines identifies candidate markers for pancreatic tumorigenesis and metastasis

Missiaglia

Blaveri

Terris

et al. 2004

Intl Journal of Cancer

139

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“…Of these, PSN-1 and SEKI were established at the National Cancer Center Research Institute (Tokyo, Japan) (Shimoyama, 1975;Yamada et al, 1986). FA-6 was a gift from N. Nagata (National Defense Medical College, Saitama, Japan) (Nagata et al, 1989). KP1N, KP2, KP3, H48N were established by A. Kono and colleagues (National Kyushu Cancer Center, Fukuoka, Japan) (Ikeda et al, 1990).…”

mentioning

confidence: 99%

Production of endothelin-1 and thrombomodulin by human pancreatic cancer cells

Oikawa¹,

Kushuhara²,

Ishikawa³

et al. 1994

Br J Cancer

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Summary Analysis of bioactive substances produced by cancer cells is one approach to understanding the biological features of human cancer. One of these bioactive substances is endothelin (ET)-1, a peptide with potent vasoconstrictive activity produced by vascular endothelial cells. We have previously reported the production of ET-1 by several types of human cancer, especially pancreatic cancer cells. To elucidate whether these cancer cells might share biological characteristics with vascular endothelial cells, we investigated the production of three ET isoforms in pancreatic cancer cells, using a specific radioimmunoassay. Further, we also investigated whether these cells produce thrombomodulin (TM), another product of endothelial cells functioning as a modulator of procoagulant activity. ET-1 was detected in 11 of 12 pancreatic cancer cell lines (92%) while ET-2 and ET-3 were detectable in only one cell line. Gel filtration analysis confirmed the presence of ET-1. Moreover, TM was detected in the cell lysates of 11 of the 12 cell lines (92%) and it was released into the culture medium in the majority (58%) of these cell lines. TM mRNA was also detected in these cells. In addition, TM was demonstrated immunocytochemically along the cell surface. These results suggest that pancreatic cancer cells share two characteristics with endothelial cells: the production of ET-1 and TM.

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“…Human tumour cell lines and their xenografts in nude mice Six human tumour cultured cell lines were studied: KEsC-2 (squamous cell oesophageal carcinoma) formerly designated as 'KN-13' (Honda et al, 1988b), (anaplastic pancreatic carcinoma) (Nagata et al, 1989), SEKI (melanoma) (Shimoyama, 1975), Lu-65A (giant cell lung carcinoma), MIA PaCa-2 (pancreatic adenocarcinoma) and PLC/PRF/5 (hepatocellular carcinoma). KEsC-2 and FA-6 were examined because they were established from patients presenting with HHM.…”

Section: Methodsmentioning

confidence: 99%

Production of parathyroid hormone-related protein in tumour xenografts in nude mice presenting with hypercalcaemia

Miyake¹,

Yamaguchi²,

Honda³

et al. 1991

Br J Cancer

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Summary This study examined the pathophysiological role of parathyroid hormone-related protein (PTHrP) in humoral hypercalcaemia of malignancy (HHM). Seven human tumour xenografts were analysed in nude mice; five tumours (KEsC-2, oesophageal carcinoma; FA-6, pancreatic carcinoma; SEKI, melanoma; Lu-65A and Lu-61, lung carcinomas) were associated with hypercalcaemia and two tumours (MIA PaCa-2, pancreatic carcinoma; PLC/PRF/5, hepatocellular carcinoma) with normocalcaemia. Northern blot analyses, radioimmunoassay and bioassay confirmed the synthesis of PTHrP-like peptides by all five tumours associated with hypercalcaemia, but not by the two associated with normocalcaemia. These observations indicated a very close relationship between the production of PTHrP and the development of HHM. Gel filtration studies of three tumour tissue extracts revealed at least two different molecules with both PTHrP-like immunological and biological activities. One peak eluted at a position between PTHrP (1-141) and cytochrome C and the other at a position identical to cytochrome C. These results suggest that PTHrP molecules with a molecular size equal to or greater than cytochrome C participate as causative agents of HHM. All five tumour xenografts caused hypercalcaemia when grown to a size of 1.5 g in nude mice. Under cell culture conditions, four original cell lines, KEsC-2, FA-6, SEKI and Lu-65A secreted 450.0, 45.0, 3.6 and 3.0 pmol of immunoreactive PTHrP/ 1.5 x 109 cells (approximately equivalent to 1.5 g wet weight) 24 h-' into their respective culture media. Since a subcutaneous infusion of 100 pmol 24 h-of PTHrP (1 -34) into nude mice was sufficient to induce significant hypercalcaemia, we speculate that PTHrP alone released from tumour cells could induce hypercalcaemia at least in the case of KEsC-2, and possibly in FA-6. With regard to other tumours associated with hypercalcaemia, further examination of PTHrP and other compounds with bone-resorbing activity in these transplantable tumours is required to obtain a better understanding of this morbidity.

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The Tumor Cells (FA-6) Established from a Pancreatic Cancer Associated with Humoral Hypercalcemia of Malignancy: A Simultaneous Production of Parathyroid Hormone-Like Activity and Transforming Growth Factor Activity.

Cited by 24 publications

References 29 publications

Analysis of gene expression in cancer cell lines identifies candidate markers for pancreatic tumorigenesis and metastasis

Analysis of gene expression in cancer cell lines identifies candidate markers for pancreatic tumorigenesis and metastasis

Production of endothelin-1 and thrombomodulin by human pancreatic cancer cells

Production of parathyroid hormone-related protein in tumour xenografts in nude mice presenting with hypercalcaemia

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