The tumor suppressor role and ceRNA network of miR-1294 in cancer

Mao, Yunan; Shen, Jinze; FANG, LI; Zhu, Feng; Duan, Shiwei

doi:10.32604/or.2022.027359

Cited by 3 publications

(1 citation statement)

References 51 publications

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“…The competing endogenous RNAs (ceRNAs) hypothesis supposes a reciprocal modulation of ceRNA transcript and of the interacting miRNAs [ 12 ]. The miRNAs targeted to the core SMGs and downstream mRNAs were predicted by DIANA-TarBase 8.0 [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

Machine learning to establish three sphingolipid metabolism genes signature to characterize the immune landscape and prognosis of patients with gastric cancer

Yan,

Yu,

et al. 2024

BMC Genomics

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Background Gastric cancer (GC) is one of the most common malignant tumors worldwide. Nevertheless, GC still lacks effective diagnosed and monitoring method and treating targets. This study used multi omics data to explore novel biomarkers and immune therapy targets around sphingolipids metabolism genes (SMGs). Method LASSO regression analysis was performed to filter prognostic and differently expression SMGs among TCGA and GTEx data. Risk score model and Kaplan-Meier were built to validate the prognostic SMG signature and prognostic nomogram was further constructed. The biological functions of SMG signature were annotated via multi omics. The heterogeneity landscape of immune microenvironment in GC was explored. qRT-PCR was performed to validate the expression level of SMG signature. Competing endogenous RNA regulatory network was established to explore the molecular regulatory mechanisms. Result 3-SMGs prognostic signature (GLA, LAMC1, TRAF2) and related nomogram were constructed combing several clinical characterizes. The expression difference and diagnostic value were validated by PCR data. Multi omics data reveals 3-SMG signature affects cell cycle and death via several signaling pathways to regulate GC progression. Overexpression of 3-SMG signature influenced various immune cell infiltration in GC microenvironment. RBP-SMGs-miRNA-mRNAs/lncRNAs regulatory network was built to annotate regulatory system. Conclusion Upregulated 3-SMGs signature are excellent predictive diagnosed and prognostic biomarkers, providing a new perspective for future GC immunotherapy.

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Section: Methodsmentioning

confidence: 99%

Machine learning to establish three sphingolipid metabolism genes signature to characterize the immune landscape and prognosis of patients with gastric cancer

Yan,

Yu,

et al. 2024

BMC Genomics

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BIRC5 facilitates cisplatin‐chemoresistance in a m⁶A‐dependent manner in ovarian cancer

Fan,

Pan,

Jia

et al. 2023

Cancer Medicine

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Cisplatin‐based chemotherapy is the standard treatment for metastatic ovarian cancer (OC). However, chemoresistance continues to pose significant clinical challenges. Recent research has highlighted the baculoviral inhibitor of the apoptosis protein repeat‐containing 5 (BIRC5) as a member of the inhibitor of the apoptosis protein (IAP) family. Notably, BIRC5, which has robust anti‐apoptotic capabilities, is overexpressed in numerous cancers. Its dysfunction has been linked to challenges in cancer treatment. Yet, the role of BIRC5 in the chemoresistance of OC remains elusive. In our present study, we observed an upregulation of BIRC5 in cisplatin‐resistant cell lines. This upregulation was associated with enhanced chemoresistance, which was diminished when the expression of BIRC5 was silenced. Intriguingly, BIRC5 exhibited a high number of N6‐methyladenosine (m6A) binding sites. The modification of m6A was found to enhance the expression of BIRC5 by recognizing and binding to the 3′‐UTR of mRNA. Additionally, the insulin‐like growth factor 2 mRNA‐binding protein 1 (IGF2BP1) was shown to stabilize BIRC5 mRNA, synergizing with METTL3 and intensifying chemoresistance. Supporting these in vitro findings, our in vivo experiments revealed that tumors were significantly smaller in size and volume when BIRC5 was silenced. This reduction was notably counteracted by co‐silencing BIRC5 and overexpressing IGF2BP1. Our results underscored the pivotal role of BIRC5 in chemoresistance. The regulation of its expression and the stability of its mRNA were influenced by m6A modifications involving both METTL3 and IGF2BP1. These insights presented BIRC5 as a promising potential therapeutic target for addressing cisplatin resistance in OC.

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Circ_0096710 facilitates tumor growth via controlling ADAM10 expression in esophageal squamous cell carcinoma

Dong,

2024

Thoracic Cancer

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BackgroundEsophageal squamous cell carcinoma (ESCC) is a global cancer related to the sixth largest cause of death. Circular RNAs (circRNAs) have affected the progress of ESCC during recent years, but the mechanism is not completely clear. So here we probed the effects of hsa_circ_0096710 (circ_0096710) in ESCC.MethodsRelative levels of circ_0096710, miR‐1294, and ADAM10 were quantified by the quantitative real‐time reverse transcription‐polymerase chain reaction in ESCC tissues. Western blot assessed ADAM10, PCNA, MMP2, VEGFA, and OCT4 protein levels. Cell proliferative capacity was assessed by cell counting and cell colony‐forming assays. Transwell assays assessed cell migration and invasion. Angiogenesis was detected by tube formation assays. Stemness of cancer cells was estimated by sphere formation assays. Dual‐luciferin reporter and RNA immunoprecipitation assays determined the targeting relationship between miR‐1294 and circ_0096710 or ADAM10.ResultsRelative levels of circ_0096710 and ADAM10 mRNA were upregulated in ESCC cells, yet miR‐1294 was downregulated. Circ_0096710 silencing repressed ESCC cell proliferation, migration, invasion, angiogenesis, and stem‐like properties. Moreover, circ_0096710 was an upstream target of miR‐1294, and miR‐1294 inhibition reversed the role of circ_0096710 downregulation in ESCC cells. Furthermore, ADAM10 was a downstream target of miR‐1294, and miR‐1294 overexpression suppressed ESCC cell proliferation, migration, invasion, angiogenesis, and stem‐like properties by targeting ADAM10. Meanwhile, circ_0096710 upgraded ADAM10 expression through sponging miR‐1294. Also, circ_0096710 downregulation restrained tumor growth in mouse models.ConclusionCirc_0096710 upregulates ADAM10 via mediating miR‐1294 expression so as to accelerate the occurrence of ESCC, suggesting that circ_0096710 may be a potential therapeutic target for ESCC.

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The tumor suppressor role and ceRNA network of miR-1294 in cancer

Cited by 3 publications

References 51 publications

Machine learning to establish three sphingolipid metabolism genes signature to characterize the immune landscape and prognosis of patients with gastric cancer

Machine learning to establish three sphingolipid metabolism genes signature to characterize the immune landscape and prognosis of patients with gastric cancer

BIRC5 facilitates cisplatin‐chemoresistance in a m⁶A‐dependent manner in ovarian cancer

Circ_0096710 facilitates tumor growth via controlling ADAM10 expression in esophageal squamous cell carcinoma

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The tumor suppressor role and ceRNA network of miR-1294 in cancer

Cited by 3 publications

References 51 publications

Machine learning to establish three sphingolipid metabolism genes signature to characterize the immune landscape and prognosis of patients with gastric cancer

Machine learning to establish three sphingolipid metabolism genes signature to characterize the immune landscape and prognosis of patients with gastric cancer

BIRC5 facilitates cisplatin‐chemoresistance in a m6A‐dependent manner in ovarian cancer

Circ_0096710 facilitates tumor growth via controlling ADAM10 expression in esophageal squamous cell carcinoma

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BIRC5 facilitates cisplatin‐chemoresistance in a m⁶A‐dependent manner in ovarian cancer