The two layer method does not improve the preservation of porcine kidneys

Hosgood, Sarah A.; Mohamed, Ismail; Nicholson, Michael L.

doi:10.12659/msm.881326

Cited by 15 publications

(12 citation statements)

References 30 publications

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“…Several approaches have been investigated to supply oxygen to the organ (reviewed in). The two‐layer preservation method using perfluorocarbons is effective in preserving different organs . Active gaseous oxygenation of the preservation solution by retrograde persufflation is also possible .…”

Section: Discussionmentioning

confidence: 99%

First-in-human use of a marine oxygen carrier (M101) for organ preservation: A safety and proof-of-principle study

Meur

Badet

Essig

et al. 2020

American Journal of Transplantation

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Funding information French Ministry of HealthThe medical device M101 is an extracellular hemoglobin featuring high oxygen-carrying capabilities. Preclinical studies demonstrated its safety as an additive to organ preservation solutions and its beneficial effect on ischemia/reperfusion injuries.OXYgen carrier for Organ Preservation (OXYOP) is a multicenter open-label study evaluating for the first time the safety of M101 added (1 g/L) to the preservation solution of one of two kidneys from the same donor. All adverse events (AEs) were analyzed by an independent data and safety monitoring board. Among the 58 donors, 38% were extended criteria donors. Grafts were preserved in cold storage (64%) or machine perfusion (36%) with a mean cold ischemia time (CIT) of 740 minutes. At 3 months, 490 AEs (41 serious) were reported, including two graft losses and two acute rejections (3.4%). No immunological, allergic, or prothrombotic effects were reported. Preimplantation and 3-month biopsies did not show thrombosis or altered microcirculation. Secondary efficacy end points showed less delayed graft function (DGF) and better renal function in the M101 group than in the contralateral kidneys.In the subgroup of grafts preserved in cold storage, Kaplan-Meier survival and Cox

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Section: Discussionmentioning

confidence: 99%

First-in-human use of a marine oxygen carrier (M101) for organ preservation: A safety and proof-of-principle study

Meur

Badet

Essig

et al. 2020

American Journal of Transplantation

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“…It has been shown that there were no significant differences in ATP content in pancreatic tissue after preservation when comparing TLM to simple cold UW organ preservation [16]. Moreover, a recent study showed that TLM did not improve the preservation condition of porcine kidneys [17]. Therefore there is a clinical need for new methods to ameliorate organ storage conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Recently it has also been shown that the TLM only oxygenates [15,16] a small volume fraction of the pancreas and therefore does not provide oxygen to all islet cells located in the organ. Moreover, the use of TLM has shown no superiority over conventional organ storage in kidneys [17].…”

Section: Introductionmentioning

confidence: 99%

Hyperbaric oxygenation of UW solution positively impacts on the energy state of porcine pancreatic tissue*

et al. 2011

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Background: Pancreatic islet transplantation is a promising option for the treatment of diabetic patients; xenotransplantation of porcine islet cells would be a possibility to overcome the shortage of donor organs. Usually the donor pancreas is preserved with University of Wisconsin (UW) solution. A large number of reports have shown that the two-layer method (TLM), which employs oxygenated perfluorochemical and UW solution, is superior to simple cold storage. However, the extensive use of TLM is cost intensive and there is evidence that TLM only oxygenates small parts of the organ preserved. Another possibility to increase the oxygen supply during organ preservation would be the use of hyperbaric oxygenation (HBO) which increases the oxygen tension in fluids. The aim of this study was to evaluate the effect of pre-oxygenation of different preservation solutions on organ quality in terms of high energy phosphate levels as well as the occurrence of apoptosis and the induction of heat shock proteins and nitrosative stress induced cell death in porcine pancreatic tissue.Methods: Porcine pancreatic tissue was preserved in different preservation solutions with or without pre-oxygenation for 6 hours of cold ischemic time (CIT). Then, tissue specimens were harvested and high energy phosphate levels were determined by HPLC. Moreover, immunohistochemistry was performed in order to detect occurrence of apoptosis, heat shock protein 70 (HSP70) as well as nitrosative stress induced cell death.Results: Organs stored in pre-oxygenated UW solution showed best results in terms of high energy phosphate levels; apoptotic cells per islet as well as HSP70 positivity were significantly less when compared to simple UW storage and all other organ preservation solutions with or without pre-oxygenation.Conclusions: Pre-oxygenation of UW solution is a simple and promising method to improve islet cell quality after cold organ storage. However, further in vitro experiments have to be performed in order to confirm these findings.

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“…The use of TLM has shown potential in other organs but has failed to gain much support as the ability of oxygen to penetrate deep into tissue in more densely capsulated organs has been questioned. In the kidney its beneficial effect was found in a rat model, however, when applied in a porcine model the results showed no advantage [121,[124][125][126].…”

Section: Oxygenated Solutionsmentioning

confidence: 99%

“…PFC can also be formulated as an emulsion for continuous perfusion and was applied during early attempts at machine perfusion [126][127][128][129]. However, the instability and adverse effects of the emulsions at that time prevented their continued application [121].…”

Section: Oxygenated Solutionsmentioning

confidence: 99%