The ubiquitin ligase XBAT32 regulates lateral root development in Arabidopsis

Nodzon, Lisa; Xu, Weihui; Wang, Yongsheng; Pi, Liya; Chakrabarty, P. K.; Song, Wenlei

doi:10.1111/j.1365-313x.2004.02266.x

Cited by 91 publications

(116 citation statements)

References 41 publications

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“…Therefore, we predicted that OsBBI1 belongs to the RING-H2 type of the RING family (Supplementary information, Figure S5). Of these, Arabidopsis BIG BROTHER, SDIR1, SIS3, RING1, NLA, SINAT5, and XBAT32, and rice EL5 and GW2 possess E3 ligase activity in vitro [38][39][40][41][42][43][44][45][46]. NLA, SIS3, SDIR1, and RING1 play roles in biotic and abiotic stress responses [40][41][42][43].…”

Section: Osbbi1 Encodes a Ring Protein With E3 Ligase Activitymentioning

confidence: 99%

Rice RING protein OsBBI1 with E3 ligase activity confers broad-spectrum resistance against Magnaporthe oryzae by modifying the cell wall defence

Zhong

et al. 2011

Cell Res

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Emerging evidence suggests that E3 ligases play critical roles in diverse biological processes, including innate immune responses in plants. However, the mechanism of the E3 ligase involvement in plant innate immunity is unclear. We report that a rice gene, OsBBI1, encoding a RING finger protein with E3 ligase activity, mediates broad-spectrum disease resistance. The expression of OsBBI1 was induced by rice blast fungus Magnaporthe oryzae, as well as chemical inducers, benzothiadiazole and salicylic acid. Biochemical analysis revealed that OsBBI1 protein possesses E3 ubiquitin ligase activity in vitro. Genetic analysis revealed that the loss of OsBBI1 function in a Tos17-insertion line increased susceptibility, while the overexpression of OsBBI1 in transgenic plants conferred enhanced resistance to multiple races of M. oryzae. This indicates that OsBBI1 modulates broad-spectrum resistance against the blast fungus. The OsBBI1-overexpressing plants showed higher levels of H 2 O 2 accumulation in cells and higher levels of phenolic compounds and cross-linking of proteins in cell walls at infection sites by M. oryzae compared with wild-type (WT) plants. The cell walls were thicker in the OsBBI1-overexpressing plants and thinner in the mutant plants than in the WT plants. Our results suggest that OsBBI1 modulates broad-spectrum resistance to blast fungus by modifying cell wall defence responses. The functional characterization of OsBBI1 provides insight into the E3 ligase-mediated innate immunity, and a practical tool for constructing broad-spectrum resistance against the most destructive disease in rice.

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Section: Osbbi1 Encodes a Ring Protein With E3 Ligase Activitymentioning

confidence: 99%

Rice RING protein OsBBI1 with E3 ligase activity confers broad-spectrum resistance against Magnaporthe oryzae by modifying the cell wall defence

Zhong

et al. 2011

Cell Res

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“…Members of several subgroups have been linked to important physiological and developmental processes, including XBAT32 (XB3 ortholog 2 in Arabidopsis), which belongs to the RING domain-containing ankyrin repeat subgroup (Nodzon et al, 2004;Stone et al, 2005). Members of this subgroup are referred to as XBAT due to their structural similarity to the rice (Oryza sativa) XB3 protein (Nodzon et al, 2004). XBAT32 has been characterized as a positive regulator of lateral root development (Nodzon et al, 2004).…”

mentioning

confidence: 99%

“…Mutations that stabilize SLR1/IAA14 prohibit the formation of lateral roots (Fukaki et al, 2005). Other E3 ligases with a role in lateral root development include the F-box proteins CEGENDUO (CEG), VIER F-BOX PROTEINE (VFB), ARABIDILLO-1 and ARABIDILLO-2, and the RING proteins SINAT5 and XBAT32 (Xie et al, 2002;Nodzon et al, 2004;Coates et al, 2006;Dong et al, 2006;Schwager et al, 2007). Plants that lack VFB and ARABIDILLO produce fewer lateral roots than wild-type plants, and an increase in the number of lateral roots is observed with loss of CEG (Coates et al, 2006;Dong et al, 2006;Schwager et al, 2007).…”

mentioning

confidence: 99%

“…The Arabidopsis RING domain-containing E3s can further be classified into 30 different subgroups based on domain architecture (Stone et al, 2005). Members of several subgroups have been linked to important physiological and developmental processes, including XBAT32 (XB3 ortholog 2 in Arabidopsis), which belongs to the RING domain-containing ankyrin repeat subgroup (Nodzon et al, 2004;Stone et al, 2005). Members of this subgroup are referred to as XBAT due to their structural similarity to the rice (Oryza sativa) XB3 protein (Nodzon et al, 2004).…”

mentioning

confidence: 99%

“…Members of this subgroup are referred to as XBAT due to their structural similarity to the rice (Oryza sativa) XB3 protein (Nodzon et al, 2004). XBAT32 has been characterized as a positive regulator of lateral root development (Nodzon et al, 2004). This E3 ligase, which is postu-lated to be involved in auxin transport, is required for the proper initiation of lateral roots.…”

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confidence: 99%

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Arabidopsis RING E3 Ligase XBAT32 Regulates Lateral Root Production through Its Role in Ethylene Biosynthesis

et al. 2010

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XBAT32, a member of the RING domain-containing ankyrin repeat subfamily of E3 ligases, was previously identified as a positive regulator of lateral root development. Arabidopsis (Arabidopsis thaliana) plants harboring a mutation in XBAT32 produce fewer lateral roots that wild-type plants. We found that xbat32 mutants produce significantly more ethylene than wild-type plants and that inhibition of ethylene biosynthesis or perception significantly increased xbat32 lateral root production. XBAT32 interacts with the ethylene biosynthesis enzymes AMINOCYCLOPROPANE-1-CARBOXYLIC ACID SYNTHASE4 (ACS4) and ACS7 in yeast-two-hybrid assays. XBAT32 is capable of catalyzing the attachment of ubiquitin to both ACS4 and ACS7 in in vitro ubiquitination assays. These results suggest that XBAT32 negatively regulates ethylene biosynthesis by modulating the abundance of ACS proteins. Loss of XBAT32 may promote the stabilization of ACSs and lead to increased ethylene synthesis and suppression of lateral root formation. XBAT32 may also contribute to the broader hormonal cross talk that influences lateral root development. While auxin treatments only partially rescue the lateral root defect of xbat32, they completely restore wild-type levels of xbat32 lateral root production when coupled with ethylene inhibition. Abscisic acid, an antagonist of ethylene synthesis/signaling, was also found to stimulate rather than inhibit xbat32 lateral root formation, and abscisic acid acts synergistically with auxin to promote xbat32 lateral root production.

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Polyamines interfere with protein ubiquitylation and cause depletion of intracellular amino acids: a possible mechanism for cell growth inhibition

et al. 2018

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Spermidine is a polyamine present in eukaryotes with essential functions in protein synthesis. At high concentrations spermidine and norspermidine inhibit growth by unknown mechanisms. Transcriptomic analysis of the effect of norspermidine on the plant Arabidopsis thaliana indicates upregulation of the response to heat stress and denatured proteins. Accordingly, these polyamines inhibit protein ubiquitylation, both in vivo (in yeast, Arabidopsis, and human Hela cells) and in vitro (with recombinant ubiquitin ligase). This interferes with protein degradation by the proteasome, a situation known to deplete cells of amino acids. Norspermidine treatment of yeast cells induces amino acid depletion, and supplementation of media with amino acids counteracts growth inhibition and cellular amino acid depletion but not inhibition of protein polyubiquitylation.

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The ubiquitin ligase XBAT32 regulates lateral root development in Arabidopsis

Cited by 91 publications

References 41 publications

Rice RING protein OsBBI1 with E3 ligase activity confers broad-spectrum resistance against Magnaporthe oryzae by modifying the cell wall defence

Rice RING protein OsBBI1 with E3 ligase activity confers broad-spectrum resistance against Magnaporthe oryzae by modifying the cell wall defence

Arabidopsis RING E3 Ligase XBAT32 Regulates Lateral Root Production through Its Role in Ethylene Biosynthesis

Polyamines interfere with protein ubiquitylation and cause depletion of intracellular amino acids: a possible mechanism for cell growth inhibition

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