The UCP2-related mitochondrial pathway participates in rhein-induced apoptosis in HK-2 cells

Mao, Yong; Zhang, Mincheng; Yang, Jiapei; Sun, Hao; Wang, Dandan; Zhang, Xiaoxia; Yu, Feng; Ji, Li

doi:10.1039/c6tx00410e

Cited by 18 publications

(8 citation statements)

References 39 publications

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“…Rhein also induced toxicity at the dosage of 6.25-50 µM in hepatocytes in vitro [185]. In another investigation, it was observed that rhein induced apoptosis in renal epithelial HK-2 cells through the uncoupling protein (UCP)-2-related mitochondrial pathway [186]. In addition, the dose-dependent effects of rhein exerted a toxic effect in hepatic HL-7702 cells via the oxidative stress-involved mitochondria-mediated apoptotic pathway.…”

Section: Toxicity Of Rheinmentioning

confidence: 99%

Therapeutic Emergence of Rhein as a Potential Anticancer Drug: A Review of Its Molecular Targets and Anticancer Properties

et al. 2020

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According to the World Health Organization (WHO), cancer is the second-highest cause of mortality in the world, and it kills nearly 9.6 million people annually. Besides the fatality of the disease, poor prognosis, cost of conventional therapies, and associated side-effects add more burden to patients, post-diagnosis. Therefore, the search for alternatives for the treatment of cancer that are safe, multi-targeted, effective, and cost-effective has compelled us to go back to ancient systems of medicine. Natural herbs and plant formulations are laden with a variety of phytochemicals. One such compound is rhein, which is an anthraquinone derived from the roots of Rheum spp. and Polygonum multiflorum. In ethnomedicine, these plants are used for the treatment of inflammation, osteoarthritis, diabetes, and bacterial and helminthic infections. Increasing evidence suggests that this compound can suppress breast cancer, cervical cancer, colon cancer, lung cancer, ovarian cancer, etc. in both in vitro and in vivo settings. Recent studies have reported that this compound modulates different signaling cascades in cancer cells and can prevent angiogenesis and progression of different types of cancers. The present review highlights the cancer-preventing and therapeutic properties of rhein based on the available literature, which will help to extend further research to establish the chemoprotective and therapeutic roles of rhein compared to other conventional drugs. Future pharmacokinetic and toxicological studies could support this compound as an effective anticancer agent.

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Section: Toxicity Of Rheinmentioning

confidence: 99%

Therapeutic Emergence of Rhein as a Potential Anticancer Drug: A Review of Its Molecular Targets and Anticancer Properties

et al. 2020

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“…In this paper, we demonstrated that the cytotoxicity of AA I was caused via apoptosis by Hoechst 33,258 staining and caspase-3. Cell apoptosis is accompanied by various morphological changes, including nuclear condensation, apoptotic bodies and DNA fragmentation [ 34 , 35 ], which was analyzed using Hoechst 33,258 staining ( Figure 2 B). Caspases are among the best characterized biochemical markers for apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Mitochondrial Uncoupling Protein 2 against Aristolochic Acid I-Induced Toxicity in HK-2 Cells

Chen

Anger

Zhang

et al. 2022

IJMS

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Aristolochic acid I (AA I) is one of the most abundant and toxic aristolochic acids that is reported to cause Aristolochic acid nephropathy (AAN). This paper was designed to assess whether mitochondrial Uncoupling Protein 2 (UCP2), which plays an antioxidative and antiapoptotic role, could protect human renal proximal tubular epithelial (HK-2) cells from toxicity induced by AA I. In this study, HK-2 cells were treated with different concentrations of AA I with or without UCP2 inhibitor (genipin). To upregulate the expression of UCP2 in HK-2 cells, UCP2-DNA transfection was performed. The cell viability was evaluated by colorimetric method using MTT. A series of related biological events such as Reactive Oxygen Species (ROS), Glutathione peroxidase (GSH-Px), and Malondialdehyde (MDA) were evaluated. The results showed that the cytotoxicity of AA I with genipin group was much higher than that of AA I alone. Genipin dramatically boosted oxidative stress and exacerbated AA I-induced apoptosis. Furthermore, the increased expression of UCP2 can reduce the toxicity of AA I on HK-2 cells and upregulation of UCP2 expression can reduce AA I-induced oxidative stress and apoptosis. In conclusion, UCP2 might be a potential target for alleviating AA I-induced nephrotoxicity.

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“…The intensity of fluorescence reasoned is proportional to the amount of hydrogen peroxide generated by the cells (Wang & Youle, 2009). Briefly, 5 × 10 5 cells/well exposed to astrakurkurol for 24 hr and as described by Mao et al, 2017, the…”

Section: Detection Of Intracellular Levels Of Rosmentioning

confidence: 99%

Anti‐cancer effect of astrakurkurol from a folklore tribal mushroom on human hepatocellular carcinoma cells via mediating cell cycle inhibition, apoptosis, and migration

Nandi

Adhikary

Acharya

2021

Journal of Food Biochemistry

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For millennia, wild mushrooms have been extensively adopted as human food particularly in Asian countries and being treasured for their texture, savor, as well as few recommended tonic and medicinal traits. Almost all medicinal mushroom concoctions are considered as tonic, which are asserted to possess favorable health effects including disease prevention and longevity without any side effects and can seemingly be utilized constantly without any distress (Ferreira et al., 2010). Although the recognition of mushrooms as functional food and as predominant source of bioactive substances with medicinal prospective has only being

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The UCP2-related mitochondrial pathway participates in rhein-induced apoptosis in HK-2 cells

Cited by 18 publications

References 39 publications

Therapeutic Emergence of Rhein as a Potential Anticancer Drug: A Review of Its Molecular Targets and Anticancer Properties

Therapeutic Emergence of Rhein as a Potential Anticancer Drug: A Review of Its Molecular Targets and Anticancer Properties

Protective Effects of Mitochondrial Uncoupling Protein 2 against Aristolochic Acid I-Induced Toxicity in HK-2 Cells

Anti‐cancer effect of astrakurkurol from a folklore tribal mushroom on human hepatocellular carcinoma cells via mediating cell cycle inhibition, apoptosis, and migration

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