The UhpA mutant of Edwardsiella piscicida enhanced its motility and the colonization in the intestine of tilapia

Chen, Jiakang; Mu, Cuimin; Ye, Tingqi; Sun, Yongcan; Luo, Qian; Wang, Xuepeng

doi:10.1016/j.fsi.2020.05.065

Cited by 11 publications

(5 citation statements)

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“…Notably, LTC 17 (which encodes the T3SS; mean r = 0.47) included also amino acid and sugar transporters, and two regulation systems ( UhpB-UhpA , CitA-CitB ). The UhpB-UhpA genes control the motility and colonization of fish pathogens 114 and, not surprisingly, the LTC was found in GFCs 46 and 47 which included known pathogenic bacteria (grouping Aeromonas and V. natriegensis genomes; Supplementary Data 3 and 8 ) 115 , 116 . LTC 17 was also found in GFCs that grouped non-pathogenic but still host-associated taxa (GFC 25, which includes V. alginolyticus , and GFC 13 grouping Enterobacteriaceae) 117 , supporting the role of this LTC (as well as of the T3SS it encodes) in interactions with a broad range of eukaryotic hosts including zooplankton, phytoplankton and fish (Fig.…”

Section: Resultsmentioning

confidence: 98%

A comparative whole-genome approach identifies bacterial traits for marine microbial interactions

et al. 2022

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Microbial interactions shape the structure and function of microbial communities with profound consequences for biogeochemical cycles and ecosystem health. Yet, most interaction mechanisms are studied only in model systems and their prevalence is unknown. To systematically explore the functional and interaction potential of sequenced marine bacteria, we developed a trait-based approach, and applied it to 473 complete genomes (248 genera), representing a substantial fraction of marine microbial communities. We identified genome functional clusters (GFCs) which group bacterial taxa with common ecology and life history. Most GFCs revealed unique combinations of interaction traits, including the production of siderophores (10% of genomes), phytohormones (3–8%) and different B vitamins (57–70%). Specific GFCs, comprising Alpha- and Gammaproteobacteria, displayed more interaction traits than expected by chance, and are thus predicted to preferentially interact synergistically and/or antagonistically with bacteria and phytoplankton. Linked trait clusters (LTCs) identify traits that may have evolved to act together (e.g., secretion systems, nitrogen metabolism regulation and B vitamin transporters), providing testable hypotheses for complex mechanisms of microbial interactions. Our approach translates multidimensional genomic information into an atlas of marine bacteria and their putative functions, relevant for understanding the fundamental rules that govern community assembly and dynamics.

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Section: Resultsmentioning

confidence: 98%

A comparative whole-genome approach identifies bacterial traits for marine microbial interactions

et al. 2022

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“…Notably, LTC 17 (which encodes the T3SS; mean r = 0.47) included also amino acid and sugar transporters, and two regulation systems ( UhpB-UhpA , CitA-CitB ). The UhpB-UhpA genes control the motility and colonization of fish pathogens 105 and, not surprisingly, the LTC was found in GFCs 46 and 47 which included known pathogenic bacteria (grouping Aeromonas and V. natriegensis genomes; Supplementary Tables 3 and 7) 106,107 . LTC 17 was also found in GFCs that grouped non-pathogenic but still host associated taxa (GFC 25, which includes V. alginolyticus and GFC 13 grouping Enterobacteriaceae) 108 , supporting the role of this LTC (as well as of the T3SS it encodes) in interactions with eukaryotic hosts.…”

Section: Resultsmentioning

confidence: 98%

Comparative whole-genome approach to identify bacterial traits for microbial interactions

Zoccarato

Sher

Miki

et al. 2020

Preprint

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ABSTRACTInteractions among microorganisms affect the structure and function of microbial communities, with potentially far-reaching effects on ecosystem health and biogeochemical cycles. The functional traits mediating microbial interactions are known for several model organisms, but the prevalence of these traits across microbial diversity is unknown. We developed a new genomic approach to systematically explore the occurrence of metabolic functions and specific interaction traits (e.g. the production of vitamins, siderophores, antimicrobial compounds and phytohormones), and apply this approach to 473 sequenced genomes from marine bacteria. We identify 48 coherent genome functional clusters (GFCs), that are partly consistent with known bacterial ecotypes (e.g. within pico-Cyanobacteria and Vibrio taxa) and identify putative new ones (e.g. Marinobacter, Alteromonas and Pseudoalteromonas). Interaction traits such as the production of and resistance towards antimicrobial compounds and the production of phytohormones are widely distributed among the GFCs, while other traits are less common (e.g. siderophores and secretion systems are found in 32% of genomes or less). Several GFCs lack the ability to produce B vitamins, suggesting that these metabolites represent essential trading goods for many bacteria. Alpha- and Gammaproteobacteria encode many interaction traits, and appear particularly poised to interact both synergistically and antagonistically with co-occurring bacteria and phytoplankton. Linked Trait Clusters (LTCs) group chemotaxis, motility and adhesion with regulatory systems involved in virulence and biofilm formation, and suggest that type-4 secretion systems may be used to inject the hormone indole acetic acid into target phytoplankton cells. Similar efficient processing and representation of multidimensional microbial functional information will be increasingly essential for translating genomes into ecosystem understanding across biomes.

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“…In this study, the uhp A gene deletion up‐regulated the gene expression of the flagellum. Importantly, the uhp A mutant could enhance its motility using a mucin penetration assay (Chen et al., 2020). Meanwhile, EsrA and EsrB are responsible for T3SS regulation in E. piscicida (Leung et al., 2019; Wang et al., 2010).…”

Section: Discussionmentioning

confidence: 99%

The role of UhpA in regulating the virulence gene expression in Edwardsiella piscicida

Chen

et al. 2020

Journal of Fish Diseases

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Edwardsiella piscicida (E. piscicida) is an important fish pathogen. However, the mechanism of Glu6P transport regulatory protein UhpA how to affect the virulence gene expression in E. piscicida is still unclear. The results in this study showed that the metabolism‐related gene expression of cysteine synthase (orf 1134) and sulphate transporter (ychM) in the uhpA mutant strain ΔuhpA was 0.76‐fold and 0.68‐fold lower than the ones in the wild strains (p < .05). The gene expression of ethA and ethB in the ΔuhpA strain was 0.80‐fold and 0.72‐fold lower than the ones in the wild strains (p < .05). However, the gene expression of fliC and flgN in the ΔuhpA was 1.51‐fold and 1.21‐fold higher than the ones in the wild strains (p < .05). The gene expression of T3SS (esrB and esrC) and T6SS (evpB and evpC) in the ΔuhpA was 1.27‐fold, 1.13‐fold, 1.28‐fold and 1.23‐fold higher than the ones in the wild strains (p < .05). This suggested that the uhpA gene could regulate the key virulence gene expression, and the uhpA gene was associated with the pathogenicity of E. piscicida in fish.

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The UhpA mutant of Edwardsiella piscicida enhanced its motility and the colonization in the intestine of tilapia

Cited by 11 publications

References 28 publications

A comparative whole-genome approach identifies bacterial traits for marine microbial interactions

A comparative whole-genome approach identifies bacterial traits for marine microbial interactions

Comparative whole-genome approach to identify bacterial traits for microbial interactions

The role of UhpA in regulating the virulence gene expression in Edwardsiella piscicida

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