The Ultrastructure Development of Rat Brain Histaminergic Neurons in Postnatal Ontogenesis

Immunoreactivity of Monoaminoxidase Type B, NeuN, Neuroglobin, and ATP Synthase in Rat Brain Histaminergic Neurons in the Dynamics of Postnatal Ontogenesis

“…During cell differentiation, ATP synthase also promotes the formation of mitochondrial cristae [27][28][29]. Our earlier electron microscopic study showed that the number of mitochondria in the cytoplasm of histaminergic neurons increases dynamically as they develop, and their cristae develops [19]. Therefore, the increase in the immunoreactivity of ATP synthase from 5th to 90th day of postnatal development of the rat hypothalamus histaminergic neurons, which we demonstrated, is in full agreement with our electron microscopic data.…”

“…Since ATP synthase is localized on the inner membrane of mitochondria, the location of these granules and clumps in the cytoplasm of histaminergic neurons apparently corresponds to the distribution of these organelles in them. This arrangement of mitochondria is indeed characteristic of histaminergic neurons in the hypothalamus of rats [19]. During the differentiation of histaminergic neurons, contact of mitochondria with the nuclear membrane is observed [19], which corresponds to the accumulation of immunopositive aggregates in the perinuclear region of the described neurocytes.…”

“…This arrangement of mitochondria is indeed characteristic of histaminergic neurons in the hypothalamus of rats [19]. During the differentiation of histaminergic neurons, contact of mitochondria with the nuclear membrane is observed [19], which corresponds to the accumulation of immunopositive aggregates in the perinuclear region of the described neurocytes. It indicates a high level of metabolic processes with significant energy costs in this zone.…”

“…In the process of postnatal development in histaminergic neurons, the nuclear apparatus is reorganized, as a result of which the number of nucleoli and the number of ribosome subunits accumulating between the nucleoli and the karyolemma decrease in the nuclei, the transition from compact nucleoli observed in 5-day-old animals to larger nucleoli of the classical reticular type, while the number of all organelles in the cytoplasm increases. Thus, there is a regular development of the functional apparatuses of the cell: energy, synthetic, as well as digestion and protection [18,19].…”