The ultrastructure of Drosophila heart cells

Lehmacher, Christine; Abeln, Bettina; Paululat, Achim

doi:10.1016/j.asd.2012.02.002

Cited by 76 publications

(136 citation statements)

References 49 publications

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“…(b) Selected features of the turquoise killifish that are conserved in human and vertebrate canonical research organisms (zebrafish and house mouse), but are missing in invertebrate canonical research organisms (round worm and fruit fly). References: nervous system: Shimeld and Holland (2000), Freeman and Doherty (2006); Lohr and Hammerschmidt (2011), Oikonomou and Shaham (2011), immune system: Langenau and Zon (2005), Engelmann and Pujol (2010), Buchon, Silverman and Cherry ( 2014); circulatory system: Lehmacher, Abeln and Paululat ( 2012); Stephenson, Adams and Vaccarezza ( 2017); respiratory system: Schottenfeld, Song and Ghabrial ( 2010); skeletal system: Shimeld and Holland (2000); muscular system: Moerman and Williams (2006), Demontis, Piccirillo, Goldberg and Perrimon ( 2013), Piccirillo, Demontis, Perrimon and Goldberg ( 2014), Goody, Carter, Kilroy, Maves and Henry ( 2017); digestive system: McKay, McKay, Avery and Graff ( 2003), Arrese and Soulages (2010), Hashmi et al. ( 2013), Kuraishi, Hori and Kurata ( 2013), Lemaitre and Miguel‐Aliaga (2013), McGhee (2013), Ritter et al.…”

Section: Research Organisms For Agingmentioning

confidence: 99%

The African turquoise killifish: A research organism to study vertebrate aging and diapause

Brunet²

2018

Aging Cell

135

118

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SummaryThe African turquoise killifish has recently gained significant traction as a new research organism in the aging field. Our understanding of aging has strongly benefited from canonical research organisms—yeast, C. elegans, Drosophila, zebrafish, and mice. Many characteristics that are essential to understand aging—for example, the adaptive immune system or the hypothalamo‐pituitary axis—are only present in vertebrates (zebrafish and mice). However, zebrafish and mice live more than 3 years and their relatively long lifespans are not compatible with high‐throughput studies. Therefore, the turquoise killifish, a vertebrate with a naturally compressed lifespan of only 4–6 months, fills an essential gap to understand aging. With a recently developed genomic and genetic toolkit, the turquoise killifish not only provides practical advantages for lifespan and longitudinal experiments, but also allows more systematic characterizations of the interplay between genetics and environment during vertebrate aging. Interestingly, the turquoise killifish can also enter a long‐term dormant state during development called diapause. Killifish embryos in diapause already have some organs and tissues, and they can last in this state for years, exhibiting exceptional resistance to stress and to damages due to the passage of time. Understanding the diapause state could give new insights into strategies to prevent the damage caused by aging and to better preserve organs, tissues, and cells. Thus, the African turquoise killifish brings two interesting aspects to the aging field—a compressed lifespan and a long‐term resistant diapause state, both of which should spark new discoveries in the field.

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Section: Research Organisms For Agingmentioning

confidence: 99%

The African turquoise killifish: A research organism to study vertebrate aging and diapause

Brunet²

2018

Aging Cell

135

118

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“…In the larva, one pair of cardiomyocytes, which is the 34th pair of cells counted from the anterior, builds the intracardiac valve that partitions the heart tube into an anterior aorta and a posterior heart chamber (Lehmacher et al, 2012;Medioni et al, 2009;Rotstein and Paululat, 2016). During metamorphosis, two additional valves differentiate, resulting in three intracardiac valves in total that subdivide the adult heart into four chambers.…”

Section: Resultsmentioning

confidence: 99%

“…Preparation of semi-thin sections and TEM was performed as described (Albrecht et al, 2011Lehmacher et al, 2012Lehmacher et al, , 2009Tögel et al, 2008).…”

Section: Immunohistochemistry and Temmentioning

confidence: 99%

“…Directionality of haemolymph flow within the Drosophila larval heart tube is regulated by two means: (i) a directional contraction wave running along the heart tube, and (ii) an intracardiac valve cell that separates the posterior heart chamber from the aorta portion (Lehmacher et al, 2012;Miller, 1950;Molina and Cripps, 2001;Rizki, 1978;Sellin et al, 2006;Wu and Sato, 2008;Zeitouni et al, 2007;present study). Experimental data on how the valves act and on the extent to which they contribute to the control of flow properties within the heart are not yet available.…”

Section: Introductionmentioning

confidence: 99%

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Formation and function of intracardiac valve cells in the Drosophila heart

Lammers

Abeln

Hüsken

et al. 2017

Journal of Experimental Biology

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Drosophila harbours a simple tubular heart that ensures haemolymph circulation within the body. The heart is built by a few different cell types, including cardiomyocytes that define the luminal heart channel and ostia cells that constitute openings in the heart wall allowing haemolymph to enter the heart chamber. Regulation of flow directionality within a tube, such as blood flow in arteries or insect haemolymph within the heart lumen, requires a dedicated gate, valve or flap-like structure that prevents backflow of fluids. In the Drosophila heart, intracardiac valves provide this directionality of haemolymph streaming, with one valve being present in larvae and three valves in the adult fly. Each valve is built by two specialised cardiomyocytes that exhibit a unique histology. We found that the capacity to open and close the heart lumen relies on a unique myofibrillar setting as well as on the presence of large membranous vesicles. These vesicles are of endocytic origin and probably represent unique organelles of valve cells. Moreover, we characterised the working mode of the cells in real time. Valve cells exhibit a highly flexible shape and, during each heartbeat, oscillating shape changes result in closing and opening of the heart channel. Finally, we identified a set of novel valve cell markers useful for future in-depth analyses of cell differentiation in wild-type and mutant animals.

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“…le filament épais de myosine, les composants du filament fin (actine, tropomyosine, troponines) mais également le récepteur à la ryanodine (RyR), la pompe calcique dépendante de l'ATP du réticulum sarcoplasmique (SERCA), les canaux ioniques possiblement impliqués dans l'activité pacemaker comme Ih/HCN (hyperpolarization-activated cyclic nucleotide modulated cation non-selective channel) [5], sont tous exprimés par les cardiomyocytes. Au niveau cellulaire, les cardiomyocytes de la mouche, comme ceux des mammifères, présentent des invaginations de la membrane plasmique formant des tubules T [9]. Ils sont mononucléés et sont connectés électriquement par des jonctions de type gap (jonctions communicantes).…”

Section: Analyse De La Fonction Cardiaque Chez La Drosophileunclassified

Pathologies et vieillissement cardiaque

Perrin

Röder

2016

Med Sci (Paris)

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The ultrastructure of Drosophila heart cells

Cited by 76 publications

References 49 publications

The African turquoise killifish: A research organism to study vertebrate aging and diapause

The African turquoise killifish: A research organism to study vertebrate aging and diapause

Formation and function of intracardiac valve cells in the Drosophila heart

Pathologies et vieillissement cardiaque

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