The Ultrastructure of Human and Murine Astrocytes and of Human Fibroblasts in Culture

Abstract

A study was undertaken to seek fine structural differences between normal and maglinant astrocytes and normal fibroblasts in cell culture. A series of human adult normal astrocytes and malignant astrocytes (including RSV-transformed cells) whose cultural characteristics had previously been reported formed the basis of the study. Also included were matched normal human fetal fibroblasts and astrocytes (from the same fetus) and methylcholanthrene-induced murine malignant astrocytes. Pellets of cells scraped from their culture vessel and sectioned as well as in situ preparations were examined. T h e fibroblasts were the only cells whose external microfibrils were collagenare-sensitive and considered to be tropocollagen. Both the benign and the malignant astrocytes had intracytoplasmic microfibril bands (gliofibrils) and some had external collagenare-resistant microfibrils. Malignant astrocytes had a much more developed membrane system (used in its widest sense) than normal astrocytes, had many free ribosomes and some had morphologically abnormal mitochondria. Virus was seen only in the niurine malignant astrocytes, was abundant and of the murine leukemia virus group. Areas interpreted as representing fusion in progress were seen at the surface of some RSV-transfomed giant tumor astrocytes. The fine structure of one cell type from an IgC-producing culture from human glioblastoma corresponded with that reported for immunocytes.The cytology and growth kinetics of human astrocytes in cell culture have been described (Ponte'n & Macintyre 1968). This series was obtained by culturing routinely all neurosurgical specimens taken over a prolonged period. These biopsies included tissues of tumor and non-tumor origin from brain. Variants transformed by Rous sarcoma virus (RSV) were derived from these cells (Ponte'n & Macintyre 1968, Macintyrp et. al. 1969. The aim of the present study has been to examine the fine structure of these same cultures, in order Received 9.ix.71

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Post‐transcriptional regulation of fukutin in an astrocytoma cell line

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