The unicellular green alga Dunaliella salina Teod. as a model for abiotic stress tolerance: genetic advances and future perspectives

Ramos, A.; Polle, Jürgen E.W.; Tran, Duc; Cushman, John C.; Jin, EonSeon; Varela, João

doi:10.4490/algae.2011.26.1.003

Cited by 95 publications

(72 citation statements)

References 170 publications

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“…The reconstructed pathway is consistent with those proposed for plants (Kim et al, 2009) and algae (Steinbrenner and Linden, 2001;Ramos et al, 2011).…”

Section: Carotenoid Biosynthetic Pathway In D Salinasupporting

confidence: 74%

Transcriptome sequencing and annotation of the halophytic microalga Dunaliella salina

Midoun

Miller

2017

J. Zhejiang Univ. Sci. B

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Abstract:The unicellular green alga Dunaliella salina is well adapted to salt stress and contains compounds (including β-carotene and vitamins) with potential commercial value. A large transcriptome database of D. salina during the adjustment, exponential and stationary growth phases was generated using a high throughput sequencing platform. We characterized the metabolic processes in D. salina with a focus on valuable metabolites, with the aim of manipulating D. salina to achieve greater economic value in large-scale production through a bioengineering strategy. Gene expression profiles under salt stress verified using quantitative polymerase chain reaction (qPCR) implied that salt can regulate the expression of key genes. This study generated a substantial fraction of D. salina transcriptional sequences for the entire growth cycle, providing a basis for the discovery of novel genes. This first full-scale transcriptome study of D. salina establishes a foundation for further comparative genomic studies.

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“…The reconstructed pathway is consistent with those proposed for plants (Kim et al, 2009) and algae (Steinbrenner and Linden, 2001;Ramos et al, 2011).…”

Section: Carotenoid Biosynthetic Pathway In D Salinasupporting

confidence: 74%

Transcriptome sequencing and annotation of the halophytic microalga Dunaliella salina

Midoun

Miller

2017

J. Zhejiang Univ. Sci. B

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“…1). На сегодняшний день в лабораторных условиях отработаны режимы интенсивного культивирова-ния D. salina, способные обеспечить высокую продуктивность культуры для наращивания существен-ных количеств её биомассы [12,14]. Однако результаты проведённых ранее экспериментов показа-ли, что скорость роста данной культуры резко снижается при уменьшении концентрации СО 2 в га-зовоздушной смеси (от 3 до 1 % v/v), поэтому выращивание данной культуры при барботировании воздухом для решения биотехнологических задач нецелесообразно.…”

Section: результаты и обсуждениеunclassified

Продуктивность микроводоросли Dunaliella salina Teod. при различных способах внесения углекислого газа в культуру

Gudvilovich

Боровков

2017

mbj

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Lack of carbon can be the main limiting factor in microalgae cultivation, that is why selection of optimum way for carbon to be injected into the culture environment for a particular species and photobioreactor is critical for its mass cultivation. D. salina culture was grown in laboratory photobioreactors under day-and-night lamplight of 15 kLx. Air was supplied with the aquarium compressor at the rate of 0.8 l·l-1·min-1. In the first test, bubbling was arranged via capillary of 4 mm diameter; in the second test — via aquarium air sprayer (plastic tube 5 cm length, 5 mm diameter, pore size not more than 0.1 mm). D. salina potential for the microalgae mass cultivation through increase of specific air-fluid phase surface without additional CO2 injection into gas-air mixture) with maximum productivity of 0.34 g of dry biomass from 1 liter per day has been shown experimentally. Average productivity of the culture when grown in the proposed regime is 1.5 times lower than in standard approved case.

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“…In addition to the salt stress were responsible for changes in gene expression events through an as yet unidentifed transcription factor or factors followed by de novo protein biosynthesis. Known salt stress-induced gene products include transcripts and proteins involved in carbon and iron assimilation as well as carotenoid biosynthesis [14,16,33]. Our previous results documented that decreased in cell growth is accompanied by carotenoid biosynthesis and lipid content of Dunaliella salina after 9 days nutrient stress [22].…”

Section: Carotenoid Content Of Dunaliella Salina A9mentioning

confidence: 99%

“…The adaptation of D. salina to salt stress can enhance rapidly the intracellular concentration of glycerol and glycine betaine, neutral lipid biosynthesis and carotenoid overaccumulation [33]. Fluctuations in medium osmolarity result in changes of plasma membrane lipid order, thus triggering activation of a protein kinase cascade and ultimately leading to conversion of starch into glycerol in the chloroplast [31].…”

Section: Carotenoid Content Of Dunaliella Salina A9mentioning

confidence: 99%

“…Unicellular green algae of the genus Dunaliella is a halophillic eukaryote, ovoid form, flagellated and lack a rigid polysaccharide wall and thus have been found to be able to rapidly change their volume and shape in response to changes in the extracellular hypo-or hyper-osmotic pressure [2,29,33]. The osmotic response process of Dunaliella under salinity stress include the changes of cell volume, intracellular ions concentration, intracellular glycerol concentration, and the expression of some saltinduced genes [13].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Osmotic Stress and Nutrient Starvation on the Growth, Carotenoid and Lipid Accumulation in <i>Dunaliella salina </i>A9

Vo¹,

Mai²,

Vu³

et al. 2017

PLANT

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Dunaliella salina A9 is unicellular green alga isolated from the saltern, Khanh Hoa province, Viet Nam.The effect of halostress and nutrient starvation was studied in this alga to estimate the growth, chlorophyll content and capacity of carotenoid and lipid accumulation. The results showed decrease in cell number and chlorophyll content as Dunaliella salina in response to a change from the optimal medium 1.5M NaCl to hypo-osmotic medium (0.5M NaCl) and hyper-osmotic medium (3.5M NaCl). We also observed decrease in cell count in nutrient starvation after 9 days of culture in MD4 medium. Salinity stress has more severe effect on the growth of Dunaliella salina A9 with greater decrease in cell number compared to nutrient starvation. The stress induced increasing carotenoid and lipid accumulation in cells. However the carotenoid and lipid accumulation in hypo-osmotic stress and the nutrient starvation were higher than in hyper-osmotic stress. The results suggested negative relationship between the growth rate, chlorophyll content and carotenoid, and lipid accumulation of Dunaliella salina under stress conditions.

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The unicellular green alga Dunaliella salina Teod. as a model for abiotic stress tolerance: genetic advances and future perspectives

Cited by 95 publications

References 170 publications

Transcriptome sequencing and annotation of the halophytic microalga Dunaliella salina

Transcriptome sequencing and annotation of the halophytic microalga Dunaliella salina

Продуктивность микроводоросли Dunaliella salina Teod. при различных способах внесения углекислого газа в культуру

Effect of Osmotic Stress and Nutrient Starvation on the Growth, Carotenoid and Lipid Accumulation in <i>Dunaliella salina </i>A9

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