The unified metabolism of Gluconacetobacter entanii in continuous and batch processes

Velasco-Bedrán, Hugo; López‐Isunza, Felipe

doi:10.1016/j.procbio.2007.05.017

Cited by 45 publications

(29 citation statements)

References 38 publications

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“…Therefore, these genes are necessary for the bacterium to feed on sucrose. It has been reported that in Gluconacetobacter representatives, sucrose cannot be transported through cell membrane and has to be hydrolyzed into glucose and fructose in the periplasm (Velasco‐Bedrán & López‐Isunza, ). However, the obtained results suggest that the majority of Komagataeibacter strains should not synthesize levan.…”

Section: Resultsmentioning

confidence: 99%

Comparative genomics of the Komagataeibacter strains—Efficient bionanocellulose producers

Ryngajłło

Kubiak

Jędrzejczak-Krzepkowska

et al. 2018

MicrobiologyOpen

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Komagataeibacter species are well‐recognized bionanocellulose ( BNC ) producers. This bacterial genus, formerly assigned to Gluconacetobacter , is known for its phenotypic diversity manifested by strain‐dependent carbon source preference, BNC production rate, pellicle structure, and strain stability. Here, we performed a comparative study of nineteen Komagataeibacter genomes, three of which were newly contributed in this work. We defined the core genome of the genus, clarified phylogenetic relationships among strains, and provided genetic evidence for the distinction between the two major clades, the K . xylinus and the K. hansenii . We found genomic traits, which likely contribute to the phenotypic diversity between the Komagataeibacter strains. These features include genome flexibility, carbohydrate uptake and regulation of its metabolism, exopolysaccharides synthesis, and the c‐di‐ GMP signaling network. In addition, this work provides a comprehensive functional annotation of carbohydrate metabolism pathways, such as those related to glucose, glycerol, acetan, levan, and cellulose. Findings of this multi‐genomic study expand understanding of the genetic variation within the Komagataeibacter genus and facilitate exploiting of its full potential for bionanocellulose production at the industrial scale.

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Section: Resultsmentioning

confidence: 99%

Comparative genomics of the Komagataeibacter strains—Efficient bionanocellulose producers

Ryngajłło

Kubiak

Jędrzejczak-Krzepkowska

et al. 2018

MicrobiologyOpen

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“…1). We suggest that this was due to the fact that sucrose could not be transported through the cell membrane and needed to be hydrolyzed in the periplasm to glucose and fructose (Velasco‐Bedran and Lopez‐Isunza 2007). Once this was achieved, only then could cellulose production commence, hence the late surge in cellulose production in the last 12 h of the 96 h fermentation experiment (Fig.…”

Section: Discussionmentioning

confidence: 99%

Influence of different carbon sources on bacterial cellulose production byGluconacetobacter xylinusstrain ATCC 53524

Mikkelsen

Flanagan

Dykes

et al. 2009

Journal of Applied Microbiology

251

116

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Aims: To determine the effect of carbon sources on cellulose produced by Gluconacetobacter xylinus strain ATCC 53524, and to characterize the purity and structural features of the cellulose produced. Methods and Results: Modified Hestrin Schramm medium containing the carbon sources mannitol, glucose, glycerol, fructose, sucrose or galactose were inoculated with Ga. xylinus strain ATCC 53524. Plate counts indicated that all carbon sources supported growth of the strain. Sucrose and glycerol gave the highest cellulose yields of 3·83 and 3·75 g l−1 respectively after 96 h fermentation, primarily due to a surge in cellulose production in the last 12 h. Mannitol, fructose or glucose resulted in consistent rates of cellulose production and yields of >2·5 g l−1. Solid state 13C CP/MAS NMR revealed that irrespective of the carbon source, the cellulose produced by ATCC 53524 was pure and highly crystalline. Scanning electron micrographs illustrated the densely packed network of cellulose fibres within the pellicles and that the different carbon sources did not markedly alter the micro‐architecture of the resulting cellulose pellicles. Conclusions: The production rate of bacterial cellulose by Ga. xylinus (ATCC 53524) was influenced by different carbon sources, but the product formed was indistinguishable in molecular and microscopic features. Significance and Impact of the Study: Our studies for the first time examined the influence of different carbon sources on the rate of cellulose production by Ga. xylinus ATCC 53524, and the molecular and microscopic features of the cellulose produced.

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“…It has to be stated that the inability to metabolize glucose under anaerobic condition in G. xylinus results from the lack of phosphofructokinase that is required for glycolysis [14], [32]. Hence, flux from F6P to T3P does not exist.…”

Section: Resultsmentioning

confidence: 99%

Revealing Differences in Metabolic Flux Distributions between a Mutant Strain and Its Parent Strain Gluconacetobacter xylinus CGMCC 2955

Zhong

Liu

et al. 2014

PLoS ONE

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A better understanding of metabolic fluxes is important for manipulating microbial metabolism toward desired end products, or away from undesirable by-products. A mutant strain, Gluconacetobacter xylinus AX2-16, was obtained by combined chemical mutation of the parent strain (G. xylinus CGMCC 2955) using DEC (diethyl sulfate) and LiCl. The highest bacterial cellulose production for this mutant was obtained at about 11.75 g/L, which was an increase of 62% compared with that by the parent strain. In contrast, gluconic acid (the main byproduct) concentration was only 5.71 g/L for mutant strain, which was 55.7% lower than that of parent strain. Metabolic flux analysis indicated that 40.1% of the carbon source was transformed to bacterial cellulose in mutant strain, compared with 24.2% for parent strain. Only 32.7% and 4.0% of the carbon source were converted into gluconic acid and acetic acid in mutant strain, compared with 58.5% and 9.5% of that in parent strain. In addition, a higher flux of tricarboxylic acid (TCA) cycle was obtained in mutant strain (57.0%) compared with parent strain (17.0%). It was also indicated from the flux analysis that more ATP was produced in mutant strain from pentose phosphate pathway (PPP) and TCA cycle. The enzymatic activity of succinate dehydrogenase (SDH), which is one of the key enzymes in TCA cycle, was 1.65-fold higher in mutant strain than that in parent strain at the end of culture. It was further validated by the measurement of ATPase that 3.53–6.41 fold higher enzymatic activity was obtained from mutant strain compared with parent strain.

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The unified metabolism of Gluconacetobacter entanii in continuous and batch processes

Cited by 45 publications

References 38 publications

Comparative genomics of the Komagataeibacter strains—Efficient bionanocellulose producers

Comparative genomics of the Komagataeibacter strains—Efficient bionanocellulose producers

Influence of different carbon sources on bacterial cellulose production byGluconacetobacter xylinusstrain ATCC 53524

Revealing Differences in Metabolic Flux Distributions between a Mutant Strain and Its Parent Strain Gluconacetobacter xylinus CGMCC 2955

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