We investigate the universal programmable unambiguous discrimination of two unknown quantum states with two copies. We first present the optimal positive-operator valued measure (POVM) elements that determine the individual success probabilities. In terms of the positivity of an inconclusive operator, we derive the average success probability, which is higher than the average success probability of discriminating two unknown states with one copy given in the reference [Bergou and Hillery, Phys. Rev. Lett. 94, 160501 (2005)].