The up-to-date strategies for the isolation and manipulation of single cells

Zhang, Xuan; Wei, Xing; Wei, Yu-Jia; Chen, Mingli; Wang, Jianhua

doi:10.1016/j.talanta.2020.121147

Cited by 25 publications

(26 citation statements)

References 79 publications

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“…Compared with traditional methods, microfluidics-based single-cell isolation techniques are more suitable for single-cell nucleic acid analysis due to the integrated and closed flow network which is ideal for combined analysis. Recent years, single-cell isolation methods applied in various biochemical applications have been discussed in a couple of reviews (Alam et al 2018 ; Zhang et al 2020 ). In this review, the typical single-cell trapping techniques used for nucleic acid analysis are concluded as size-based trapping, hydrodynamic trapping, droplet-based trapping, and other trapping methods based on their working mechanisms.…”

Section: Single-cell Nucleic Acid Analysis Based On Microfluidicsmentioning

confidence: 99%

Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics

Liu

Dong

et al. 2021

Microfluid Nanofluid

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Single-cell nucleic acid analysis aims at discovering the genetic differences between individual cells which is well known as the cellular heterogeneity. This technology facilitates cancer diagnosis, stem cell research, immune system analysis, and other life science applications. The conventional platforms for single-cell nucleic acid analysis more rely on manual operation or bulky devices. Recently, the emerging microfluidic technology has provided a perfect platform for single-cell nucleic acid analysis with the characteristic of accurate and automatic single-cell manipulation. In this review, we briefly summarized the procedure of single-cell nucleic acid analysis including single-cell isolation, single-cell lysis, nucleic acid amplification, and genetic analysis. And then, three representative microfluidic platforms for single-cell nucleic acid analysis are concluded as valve-, microwell-, and droplet-based platforms. Furthermore, we described the state-of-the-art integrated single-cell nucleic acid analysis systems based on the three platforms. Finally, the future development and challenges of microfluidics-based single-cell nucleic acid analysis are discussed as well.

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Section: Single-cell Nucleic Acid Analysis Based On Microfluidicsmentioning

confidence: 99%

Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics

Liu

Dong

et al. 2021

Microfluid Nanofluid

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“…The main advantage is the possibility of using traditional LC/GC-MS-based platforms providing high throughput, optimal sensitivity, and coverage due to the chromatographic separation. The main limitations are imposed by the chosen cell sampling technique [24][25][26]. Some particularly exciting works include applications of laser microdissection (LMD)-based techniques such as laser microdissection and pressure catapulting (LMPC) and laser capture microdissection (LCM) [18], as well as fluorescence-activated cell sorting (FACS) [27].…”

Section: Single-cell and Single-cell-type Metabolomicsmentioning

confidence: 99%

Plant Single-Cell Metabolomics—Challenges and Perspectives

Souza

Borghi

Fernie

2020

IJMS

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Omics approaches for investigating biological systems were introduced in the mid-1990s and quickly consolidated to become a fundamental pillar of modern biology. The idea of measuring the whole complement of genes, transcripts, proteins, and metabolites has since become widespread and routinely adopted in the pursuit of an infinity of scientific questions. Incremental improvements over technical aspects such as sampling, sensitivity, cost, and throughput pushed even further the boundaries of what these techniques can achieve. In this context, single-cell genomics and transcriptomics quickly became a well-established tool to answer fundamental questions challenging to assess at a whole tissue level. Following a similar trend as the original development of these techniques, proteomics alternatives for single-cell exploration have become more accessible and reliable, whilst metabolomics lag behind the rest. This review summarizes state-of-the-art technologies for spatially resolved metabolomics analysis, as well as the challenges hindering the achievement of sensu stricto metabolome coverage at the single-cell level. Furthermore, we discuss several essential contributions to understanding plant single-cell metabolism, finishing with our opinion on near-future developments and relevant scientific questions that will hopefully be tackled by incorporating these new exciting technologies.

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“…As the key step for single-cell analysis, single-cell isolation has attracted great attention, and a number of techniques have been developed for single-cell isolation and manipulation [29]. Limiting dilution [30] and fluorescence-activated cell sorting (FACS) [31] are two widely used techniques for single-cell isolation [32].…”

Section: Introductionmentioning

confidence: 99%

“…Limiting dilution [30] and fluorescence-activated cell sorting (FACS) [31] are two widely used techniques for single-cell isolation [32]. However, the low efficiency of the limiting dilution and the special equipment as well as the professional experience required for FACS limit their applications [29,32]. The development of single-cell printing has helped alleviate the situation, which utilizes various microfluidic technologies for single-cell isolation and analysis, such as droplet microfluidics [6,33,34], microwell arrays [35,36], and hydrodynamic traps [37,38].…”

Section: Introductionmentioning

confidence: 99%

Advances in Single-Cell Printing

Zhou¹,

Wu²,

Wen³

et al. 2022

Micromachines

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Single-cell analysis is becoming an indispensable tool in modern biological and medical research. Single-cell isolation is the key step for single-cell analysis. Single-cell printing shows several distinct advantages among the single-cell isolation techniques, such as precise deposition, high encapsulation efficiency, and easy recovery. Therefore, recent developments in single-cell printing have attracted extensive attention. We review herein the recently developed bioprinting strategies with single-cell resolution, with a special focus on inkjet-like single-cell printing. First, we discuss the common cell printing strategies and introduce several typical and advanced printing strategies. Then, we introduce several typical applications based on single-cell printing, from single-cell array screening and mass spectrometry-based single-cell analysis to three-dimensional tissue formation. In the last part, we discuss the pros and cons of the single-cell strategies and provide a brief outlook for single-cell printing.

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The up-to-date strategies for the isolation and manipulation of single cells

Cited by 25 publications

References 79 publications

Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics

Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics

Plant Single-Cell Metabolomics—Challenges and Perspectives

Advances in Single-Cell Printing

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