The uptake and subsequent loss of beryllium by rat liver parenchymal and non-parenchymal cells after the intravenous administration of particulate and soluble forms

Skilleter, David N.; Price, Roger J.

doi:10.1016/0009-2797(78)90116-3

Cited by 25 publications

(7 citation statements)

References 20 publications

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“…At the indicated times cells were removed from the culture dishes by the trypsinization procedure described above, prior to dispersion in 5 ml of filtered (0.22,um) of Be2+ necessary to cause cytotoxicity to liver cells both in vivo (Skilleter & Price, 1978) and in vitro (Skilleter & Paine, 1979). Table 2 shows that the effects of Be2+ on cell division were also consistent with the inhibitory effects seen on the increase in total cell protein and DNA synthesis during the 24 h incubation period.…”

Section: Cytofluorometric Cell Cycle Analysissupporting

confidence: 58%

Specific G1-S phase cell cycle block by beryllium as demonstrated by cytofluorometric analysis

Skilleter¹,

Price²,

Legg³

1983

Biochemical Journal

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Section: Cytofluorometric Cell Cycle Analysissupporting

confidence: 58%

Specific G1-S phase cell cycle block by beryllium as demonstrated by cytofluorometric analysis

Skilleter¹,

Price²,

Legg³

1983

Biochemical Journal

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“…Carrier-free '09CdC12 (specific radioactivity 8.51 MBq/ml, < 1 ug of Cd2+/ml) was purchased from The Radiochemical Centre, Amersham, Bucks., U.K. All other chemicals were obtained from sources detailed previously (Cain & Holt, 1979;Skilleter & Price, 1978).…”

Section: Methodsmentioning

confidence: 99%

“…as CdCl2,2JH20 and labelled with "09CdCl2. At the indicated times animals were anaesthetized with 7.5mg of Sagatal/lOOg body wt., and liver parenchymal and non-parenchymal cells were prepared by the method of Seglen (1973) as described previously (Skilleter & Price, 1978). Briefly, the method uses a collagenase-perfusion technique to produce a dispersed liver preparation known as the initial cell suspension.…”

Section: Animal Injections and Cell-purification Techniquesmentioning

confidence: 99%

Selective uptake of cadmium by the parenchyumal cells of liver

Cain¹,

Skilleter²

1980

Biochemical Journal

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“…Parenchymal and non-parenchymal cells were isolated by a modified collagenase liver perfusion technique (Skilleter and Price, 1978). Briefly, the hepatic portal vein in anaesthetized normal adult rats was cannulated with a 16-gauge i.v.…”

Section: Isolation and Culture Of Liver Parenchymal And Non-parenchymmentioning

confidence: 99%

The mechanism of hepatic uptake of a radiolabelled monoclonal antibody

Boyle

Paine

Mather

1992

Intl Journal of Cancer

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Clinical and experimental scintigraphic studies have found that radiolabelled antibodies are not only taken up by tumour(s) but also by normal liver. The accumulation of radionuclides in this organ poses a major problem to the use of radiolabelled antibodies as diagnostic and therapeutic tools. In an attempt to understand the mechanism of hepatic uptake and clearance of radiolabelled antibodies, the intrahepatic biodistribution of an 111In-labelled MAb (HMFG1), was determined following i.v. administration to normal male rats. Two hours after administration the liver contained 15% of the injected dose, with most of the remaining radioactivity in the blood. The hepatic burden of the 111In MAb remained constant over the next 72 hr in the face of decreasing blood levels of radioactivity as well as its urinary and faecal excretion. At 2 and 72 hr after injection, 50% and 10% respectively of the hepatic radiolabel was due to blood borne antibody. Following a collagenase-cell isolation procedure, only 23% of the amount remaining in the liver at 2 hr was found to be cell-associated; 66% was lost during the cell isolation and purification procedure. Cellular uptake increased with time so that, by 72 hr after administration, 58% was cell-associated and 29% freely removable. At all timepoints, the parenchymal cells contained more activity than non-parenchymal cells. No evidence of antibody-receptor interactions could be obtained either in vivo or in cultures of hepatic parenchymal and non-parenchymal cells. Our data suggest that the bulk of the hepatic burden of 111In MAb results from extravascular pooling of the antibody.

show abstract

The uptake and subsequent loss of beryllium by rat liver parenchymal and non-parenchymal cells after the intravenous administration of particulate and soluble forms

Cited by 25 publications

References 20 publications

Specific G1-S phase cell cycle block by beryllium as demonstrated by cytofluorometric analysis

Specific G1-S phase cell cycle block by beryllium as demonstrated by cytofluorometric analysis

Selective uptake of cadmium by the parenchyumal cells of liver

The mechanism of hepatic uptake of a radiolabelled monoclonal antibody

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