The uptake of 111In in the liver and bone marrow of partially hepatectomized and venesectioned rats

Ohtake, Yosuke; Maruko, Akiko; Satoh, Shinsuke; Ohkubo, Yasuhito

doi:10.1016/j.apradiso.2008.02.057

Cited by 4 publications

(3 citation statements)

References 11 publications

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“…administration. [37][38][39] This statement is consistent with the previously reported mouse serum stability tests for 111 In-labelled micelles equivalent to the ones used in our experiments. 31 Fig.…”

Section: In Vivo Biodistribution Of Ps-peo Micellessupporting

confidence: 93%

In vivo biodistribution of stable spherical and filamentous micelles probed by high-sensitivity SPECT

et al. 2016

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Understanding how nanoparticle properties such as size, morphology and rigidity influence their circulation time and biodistribution is essential for the development of nanomedicine therapies. Herein we assess the influence of morphology on cellular internalization, in vivo biodistribution and circulation time of nanocarriers using polystyrene-b-poly(ethylene oxide) micelles of spherical and elongated morphology. The glassy nature of polystyrene guarantees the morphological stability of the carriers in vivo and by encapsulating Indium-111 in their core, an assessment of the longitudinal in vivo biodistribution of the particles in healthy mice is performed with single photon emission computed tomography imaging. Our results show prolonged blood circulation, longer than 24 hours, for all micelle morphologies studied. Dynamics of micelle accumulation in the liver and other organs of the reticuloendothelial system show a size-dependent nature and late stage liver clearance is observed for the elongated morphology. Apparent contradictions between recent similar studies can be resolved by considering the effects of flexibility and degradation of the elongated micelles on their circulation time and biodistribution.

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Section: In Vivo Biodistribution Of Ps-peo Micellessupporting

confidence: 93%

In vivo biodistribution of stable spherical and filamentous micelles probed by high-sensitivity SPECT

et al. 2016

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“…It is known that polymeric micelles and small nanoparticles with DTPA- 111 In chelators can follow rapid loss of 111 In and/or DTPA- 111 In complexes after i.v. administration. − In the first case, 111 In in the form of free ions will rapidly bind to blood protein transferrin, , which will result in rapid accumulation and long retention of indium in the bone marrow and the liver, , accompanied by relatively low clearance via renal filtration. Therefore, the observed initial renal clearance in our work is not related to dissociation of 111 In ions from the carriers.…”

Section: Discussionmentioning

confidence: 99%

“…In in the form of free ions will rapidly bind to blood protein transferrin 23,24 which will result in rapid accumulation and long retention of indium in the bone marrow and the liver, 26,27 accompanied by relatively low clearance via renal filtration. Therefore, the observed initial renal clearance in our work is not related to dissociation of…”

mentioning

confidence: 99%

SPECT/CT Imaging of Pluronic Nanocarriers with Varying Poly(ethylene oxide) Block Length and Aggregation State

et al. 2016

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Optimal biodistribution and prolonged circulation of nanocarriers improves diagnostic and therapeutic effects of EPR-based nanomedicines. Despite extensive use of Pluronics in polymer-based pharmaceuticals, the influence of different PEO block length and aggregation state on the biodistribution of the carriers is rather unexplored. In this work, we studied these effects by evaluating the biodistribution of Pluronic unimers and cross-linked micelles with different PEO block size. In vivo biodistribution of 111 In-radiolabeled Pluronic nanocarriers was performed in healthy mice using SPECT/CT.All carriers show fast uptake in the organs from the reticuloendothelial system followed by a steady elimination through the hepatobiliary tract and renal filtration. The PEO block length affects the initial renal clearance of the compounds and the overall liver uptake. The aggregation state influences the long-term accumulation of the nanocarriers in the liver.We showed that the circulation time and elimination pathways can be tuned by varying the physicochemical properties of Pluronic copolymers. Our results can be beneficial for the design of future Pluronic-based nanomedicines.

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New interplay between interstitial and alveolar macrophages explains pulmonary alveolar proteinosis (PAP) induced by indium tin oxide particles

et al. 2018

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Occupational exposure to indium tin oxide (ITO) particles has been associated with the development of severe lung diseases, including pulmonary alveolar proteinosis (PAP). The mechanisms of this lung toxicity remain unknown. Here, we reveal the respective roles of resident alveolar (Siglec-F AM) and recruited interstitial (Siglec-F IM) macrophages contributing in concert to the development of PAP. In mice treated with ITO particles, PAP is specifically associated with IL-1α (not GM-CSF) deficiency and Siglec-F AM (not Siglec-F IM) depletion. Mechanistically, ITO particles are preferentially phagocytosed and dissolved to soluble In by Siglec-F IM. In contrast, Siglec-F AM weakly phagocytose or dissolve ITO particles, but are sensitive to released In through the expression of the transferrin receptor-1 (TfR1). Blocking pulmonary Siglec-F IM recruitment in CCR2-deficient mice reduces ITO particle dissolution, In release, Siglec-F AM depletion, and PAP formation. Restoration of IL-1-related Siglec-F AM also prevented ITO-induced PAP. We identified a new mechanism of secondary PAP development according to which metal ions released from inhaled particles by phagocytic IM disturb IL-1α-dependent AM self-maintenance and, in turn, alveolar clearance.

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The uptake of 111In in the liver and bone marrow of partially hepatectomized and venesectioned rats

Cited by 4 publications

References 11 publications

In vivo biodistribution of stable spherical and filamentous micelles probed by high-sensitivity SPECT

In vivo biodistribution of stable spherical and filamentous micelles probed by high-sensitivity SPECT

SPECT/CT Imaging of Pluronic Nanocarriers with Varying Poly(ethylene oxide) Block Length and Aggregation State

New interplay between interstitial and alveolar macrophages explains pulmonary alveolar proteinosis (PAP) induced by indium tin oxide particles

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