The uptake of fibronectin or fibronectin degradation products by the nuclei of cultured cells

Savill, Corinne M.; Ayad, Shirley

doi:10.1042/bst0110405

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, the rationale behind the association of fibronectin with nuclear fraction of necrotic cells remains unclear. Literature is also silent on this aspect, but a study by Savill and Ayad suggested that fibronectin or fibronectin degrada-tion products could be taken up by the nuclei of cultured cells [34].…”

Section: Discussionmentioning

confidence: 99%

Positron Emission Tomography (PET) with ¹⁸F-FGA for Diagnosis of Myocardial Infarction in a Coronary Artery Ligation Model

et al. 2022

View full text Add to dashboard Cite

Location and extent of necrosis are valuable information in the management of myocardial infarction (MI). Methods. We investigated 2-deoxy-2-18F-fluoro glucaric acid (FGA), a novel infarct-avid agent, for positron emission tomography (PET) of MI. We synthesized FGA from commercially available 18F-fluoro-2-deoxy-2-D-glucose (FDG). MI was induced in mice by permanently occluding the left anterior descending coronary artery. Biodistribution of FGA was assessed 1 h after FGA injection (11 MBq). PET/CT was conducted 1 h, 6 h, 1 d, 3 d, and 4 d after MI. Subcellular compartment of FGA accumulation in necrosis was studied by tracing the uptake of biotin-labeled glucaric acid with streptavidin-HRP in H2O2-treated H9c2 cardiomyoblasts. Streptavidin-reactive protein bands were identified by LC-MS/MS. Results. We obtained a quantitative yield of FGA from FDG within 7 min ( radiochemical purity > 99 % ). Cardiac uptake of FGA was significantly higher in MI mice than that in control mice. Imaging after 1 h of FGA injection delineated MI for 3 days after MI induction, with negligible background signal from surrounding tissues. Myocardial injury was verified by tetrazolium staining and plasma troponin (47.63 pg/mL control versus 311.77 pg/mL MI). In necrotic H9c2 myoblasts, biotinylated glucaric acid accumulated in nuclear fraction. LC-MS/MS primarily identified fibronectin in necrotic cells as a putative high fidelity target of glucaric acid. Conclusion. FGA/PET detects infarct early after onset of MI and FGA accumulation in infarct persists for 3 days. Its retention in necrotic cells appears to be a result of interaction with fibronectin that is known to accumulate in injured cardiac tissue.

show abstract

Section: Discussionmentioning

confidence: 99%

Positron Emission Tomography (PET) with ¹⁸F-FGA for Diagnosis of Myocardial Infarction in a Coronary Artery Ligation Model

et al. 2022

View full text Add to dashboard Cite

show abstract

The uptake of fibronectin or fibronectin degradation products by the nuclei of cultured cells

Cited by 1 publication

References 0 publications

Positron Emission Tomography (PET) with ¹⁸F-FGA for Diagnosis of Myocardial Infarction in a Coronary Artery Ligation Model

Positron Emission Tomography (PET) with ¹⁸F-FGA for Diagnosis of Myocardial Infarction in a Coronary Artery Ligation Model

Contact Info

Product

Resources

About

The uptake of fibronectin or fibronectin degradation products by the nuclei of cultured cells

Cited by 1 publication

References 0 publications

Positron Emission Tomography (PET) with 18F-FGA for Diagnosis of Myocardial Infarction in a Coronary Artery Ligation Model

Positron Emission Tomography (PET) with 18F-FGA for Diagnosis of Myocardial Infarction in a Coronary Artery Ligation Model

Contact Info

Product

Resources

About

Positron Emission Tomography (PET) with ¹⁸F-FGA for Diagnosis of Myocardial Infarction in a Coronary Artery Ligation Model

Positron Emission Tomography (PET) with ¹⁸F-FGA for Diagnosis of Myocardial Infarction in a Coronary Artery Ligation Model