The ura5 gene of the ascomycete Sordaria macrospora: molecular cloning, characterization and expression in Escherichia coli

Chevanton, Landry Le; Leblon, Gérard

doi:10.1016/0378-1119(89)90357-0

Cited by 26 publications

(5 citation statements)

References 28 publications

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“…The flanking regions were compared with the two previously described fungal URA5 genes from Podospora anserina (26) and Sordaria macrospora (16), and no significant similarities were found. A canonical TATA box was present at -342, and potential CAAT boxes were present at -290 and -141.…”

Section: Resultsmentioning

confidence: 99%

“…The genomic library and mock-transfected controls did not give rise to any Ura+ colonies. Plasmid DNA was isolated from eight of the Ura+ cDNA transfectants, and all contained a -700-bp EcoRI insert consistent with the expected size of a full-length OMPPase cDNA (based on the sizes of the Podospora anserina and Sordaria macrospora OMPPase genes [16,26]). Transformation of these plasmid DNAs back into CGSC 4501 and plating to uracil-depleted medium confirmed that uracil prototrophy was truly plasmid mediated.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation.

Edman¹,

Kwon-Chung²

1990

Mol. Cell. Biol.

191

177

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A cDNA encoding Cryptococcus neoformans orotidine monophosphate pyrophosphorylase (OMPPase) has been isolated by complementation of the cognate Escherichia coli pyrE mutant. The cDNA was used as a probe to isolate a genomic DNA fragment encoding the OMPPase gene (URA5). By using electroporation for the introduction of plasmid DNA containing the URA5 gene, C. neoformans ura5 mutants could be transformed at low efficiency. Ura+ transformants obtained with supercoiled plasmids containing the URA5 gene showed marked mitotic instability and contained extrachromosomal URA5 sequences, suggesting limited ability to replicate within C. neoformans. Transformants obtained with linear DNA were of two classes: stable transformants with integrated URA5 sequences, and unstable transformants with extrachromosomal URA5 sequences.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation.

Edman¹,

Kwon-Chung²

1990

Mol. Cell. Biol.

191

177

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“…To our knowledge this is the first gene cloned from S.fimicola, and the second from any Sordaria species. The uraS gene was cloned from S.macrospora (Le Chevanton, 1989) and analysis of the putative ORF revealed similarities to N. crassa codon usage in highly expressed genes [that is, a bias against purines, especially A, in the third position (Orbach et al, 1986;Chary et al, 1990)]. frq mRNA, however, rather than being abundant, can be expressed weakly under conditions where the clock is robust and active, and FRQ protein, similarly, appears to be scarce under all culture conditions (N.Garceau and J.C. Dunlap, unpublished data).…”

Section: Discussionmentioning

confidence: 99%

Intergeneric complementation of a circadian rhythmicity defect: phylogenetic conservation of structure and function of the clock gene frequency.

Merrow

Dunlap

1994

The EMBO Journal

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The Neurospora crassa frequency locus encodes a 989 amino acid protein that is a central component, a state variable, of the circadian biological clock. We have determined the sequence of all or part of this protein and surrounding regulatory regions from additional fungi representing three genera and report that there is distinct, preferential conservation of the frequency open reading frame (ORF) as compared with non‐coding sequences. Within the coding region, many of the domain hallmarks of the N. crassa protein are highly conserved, especially an internal region bearing the causative mutations in frq1 and frq7, the most extreme alleles in the frequency allelic series. Despite considerable diversity among the strains analyzed in terms of morphology, growth, circadian clock output and frq sequence, the ORF from the most distantly related fungus included in this study (Sordaria fimicola) rescues rhythmicity in a N.crassa frequency null strain. Both sequence conservation, and the ability of frequency from a genus displaying one developmental program to complement circadian defects in a separate genus with a distinct, clock‐regulated developmental program, are consistent with a central role of the frequency gene product in a general circadian oscillator capable of controlling diverse outputs in a variety of systems.

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“…1, Fig. 4 illustrates the similarities between the enzymes of Saccharomyces cereoisiae [15], Podospora anserina [16], Dictyo~telium discoideum [17], Sordaria mocrospora [18] and E, coil [19], Fr¢ quent large conserved stretches of amino acids confirmed that the L, plantarum insert corresponds to the pyre gent. The percentages of simi-larity between the L plantarum protein and those of other organisms are respectively 25.6% with S. cerevisiae, 26,4% with P. anserina, 26.8% with D. discoideum, 22.1% with S, macrospora and 21,2~ with E, coll.…”

Section: Dna Sequencementioning

confidence: 99%

Cloning and structure of the pyrE gene of Lactobacillus plantarum CCM 1904

Bouia

Bringel

Frey

et al. 1990

FEMS Microbiology Letters

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The pyrE gene of Lactobacillus plantarum CCM 1904, coding for the orotate phosphoribosyl transferase involved in the pyrimidine biosynthetic pathway, was cloned in Escherichia coli and sequenced. The predicted polypeptide sequence extending over 212 amino acids (MW 22,690) was compared to those of E. coli and to those of lower eukaryotes (Saccharomyces cerevisiae, Podospora anserina, Sordaria macrospora, Dictyostelium discoideum). Important conserved stretches were revealed, implying that these proteins are closely related.

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The ura5 gene of the ascomycete Sordaria macrospora: molecular cloning, characterization and expression in Escherichia coli

Cited by 26 publications

References 28 publications

Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation.

Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation.

Intergeneric complementation of a circadian rhythmicity defect: phylogenetic conservation of structure and function of the clock gene frequency.

Cloning and structure of the pyrE gene of Lactobacillus plantarum CCM 1904

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