2009
DOI: 10.1128/jb.00613-09
|View full text |Cite
|
Sign up to set email alerts
|

The Uracil DNA Glycosylase UdgB of Mycobacterium smegmatis Protects the Organism from the Mutagenic Effects of Cytosine and Adenine Deamination

Abstract: Spontaneous hydrolytic deamination of DNA bases represents a considerable mutagenic threat to all organisms, particularly those living in extreme habitats. Cytosine is readily deaminated to uracil, which base pairs with adenine during replication, and most organisms encode at least one uracil DNA glycosylase (UDG) that removes this aberrant base from DNA with high efficiency. Adenine deaminates to hypoxanthine approximately 10-fold less efficiently, and its removal from DNA in vivo has to date been reported to… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

1
12
0

Year Published

2010
2010
2022
2022

Publication Types

Select...
5
2

Relationship

0
7

Authors

Journals

citations
Cited by 19 publications
(13 citation statements)
references
References 37 publications
1
12
0
Order By: Relevance
“…However, its effect was synergistic with that of the ung 2 strain and led to a remarkable enhancement in the mutation rates from approximately eightfold (for the ung 2 strain) to approximately 19-fold (for the ung 2 / udgB 2 strain) compared with the wild-type background. Consistent with these observations, while the mutation spectra of the ung 2 and the double-knockout (ung 2 / udgB 2 ) strains were markedly altered from that of the wild-type strain (Table 3), that of the udgB 2 strain had a significant background of the wild-type spectrum but showed a smaller increase in AT to GC mutations, suggesting that UdgB is more important for excision of the adenosine deamination product (Hx), which is consistent with a recent study in M. smegmatis (Wanner et al, 2009). However, our findings on the overall mutation rate of the udgB 2 strain are at a variance with this report, in which the mutation frequency for the UdgBdeficient strain was found to increase~8.1-fold above the wild-type background and to be very similar to that of the Ung-deficient strain (~7.4-fold).…”
Section: Discussionsupporting
confidence: 78%
See 1 more Smart Citation
“…However, its effect was synergistic with that of the ung 2 strain and led to a remarkable enhancement in the mutation rates from approximately eightfold (for the ung 2 strain) to approximately 19-fold (for the ung 2 / udgB 2 strain) compared with the wild-type background. Consistent with these observations, while the mutation spectra of the ung 2 and the double-knockout (ung 2 / udgB 2 ) strains were markedly altered from that of the wild-type strain (Table 3), that of the udgB 2 strain had a significant background of the wild-type spectrum but showed a smaller increase in AT to GC mutations, suggesting that UdgB is more important for excision of the adenosine deamination product (Hx), which is consistent with a recent study in M. smegmatis (Wanner et al, 2009). However, our findings on the overall mutation rate of the udgB 2 strain are at a variance with this report, in which the mutation frequency for the UdgBdeficient strain was found to increase~8.1-fold above the wild-type background and to be very similar to that of the Ung-deficient strain (~7.4-fold).…”
Section: Discussionsupporting
confidence: 78%
“…The family 1 UDGs, also known as Ung, excise U from both ssDNA and dsDNA, and possess two highly conserved sequences, GQDPY (motif A) and HPSPLS (motif B) (Krokan et al, 1997). Family 5 UDGs, also known as UdgB, are restricted to a limited number of organisms that survive in extreme habitats (Sartori et al, 2002;Starkuviene & Fritz, 2002;Wanner et al, 2009), excise uracil from dsDNA and possess conserved sequences, GLAPA and HPSPLNV (HPSQQN in M. tuberculosis) as motif A and motif B, respectively. In addition to uracil, the UdgB proteins excise 5-hydroxymethyl-uracil, ethenocytosine, 5-fluorouracil and Hx from dsDNA (Srinath et al, 2007;Sartori et al, 2002).…”
Section: Introductionmentioning
confidence: 99%
“…Therefore, family 5 UDGb is a versatile repair enzyme that can deal with multiple types of deaminated base lesions. Interestingly, family 5 UDGb is implicated in playing a role in the repair of hypoxanthine in vivo in addition to its role in the repair of uracil (14). Recently, we reported on a new family of enzymes in the UDG superfamily that act as hypoxanthine DNA glycosylases rather than uracil DNA glycosylases (13).…”
Section: Discussionmentioning
confidence: 99%
“…The family 5 UDG enzymes possess repair activity on both G/U and A/U base pairs. The physiological role of the UDG in removing uracil from G/U base pairs has been studied (14,18). However, whether the UDG activity of UDGb on A/U base pairs can remove uracil in vivo remained unclear.…”
Section: Inverse Correlation Of Viability and The Udg Activity On A/umentioning
confidence: 99%
See 1 more Smart Citation