The urinary bladder epithelium of the rat

Malczak, H. T.

doi:10.1159/000145285

Cells Tissues Organs

1980

DOI: 10.1159/000145285

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The urinary bladder epithelium of the rat

H. T. Malczak¹

Abstract: The surface of the urinary bladder of the rat appeared to have a very well-ordered arrangement when viewed with the help of the scanning electron microscope. Intercellular junctions were well demarcated by prominent ridges. The majority of cells appeared to have a pentagonal or hexagonal shape. The surface morphology of the epithelial cells appeared to be consistent throughout the whole population of cells. It was made up of small ridges and some short microvilli. Comments on the significance of the surface sp… Show more

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2001

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Expansion and Long-Term Culture of Differentiated Normal Rat Urothelial Cells in Vitro

Zhang

Ludwikowski

Hurst

et al. 2001

In Vitro Cell Dev Biol Anim

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The objective of this study is to establish a reliable cell culture system for the long-term culture of rat urothelial cells (RUC), in which the cells multiply in vitro and form stratified polarized urothelium. Urothelial cells were harvested by the enzymatic digestion of the urothelium exposed by the eversion of resected rat bladders. Primary cultures were initiated in keratinocyte serum-free medium (KSFM) for selective proliferation of urothelial cells. Subsequently, the cells were propagated in a mixture of conditioned medium (CM) derived from Swiss 3T3 cell culture supernatant and KSFM (CM-KSFM). Mean population doubling time was 13.8 +/- 0.9 h. RUC were successfully maintained for 18 passages over a period of 4-5 mo. Detailed investigations of culture conditions showed that CM-KSFM yielded a differentiated multilayer structure. The stratified urothelial sheets measuring 4 x 6 cm2 could be formed and then detached using dispase. Cytokeratin pattern in both the cultured urothelial monolayer and engineered stratified layers was similar to those seen in vivo, as assessed with monoclonal antibody against cytokeratin 17. Ultrastructural morphology showed microvilli, basal cell layer, and desmosomes between adjacent cells in the stratified urothelium.

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Expansion and Long-Term Culture of Differentiated Normal Rat Urothelial Cells in Vitro

Zhang

Ludwikowski

Hurst

et al. 2001

In Vitro Cell Dev Biol Anim

View full text Add to dashboard Cite

show abstract

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The urinary bladder epithelium of the rat

Cited by 1 publication

References 4 publications

Expansion and Long-Term Culture of Differentiated Normal Rat Urothelial Cells in Vitro

Expansion and Long-Term Culture of Differentiated Normal Rat Urothelial Cells in Vitro

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