The use of a library of industrial materials to determine the nature of substrate-dependent performance of primary adherent human cells

Ni, Ming; Zimmermann, Philipp K.; Kandasamy, Karthikeyan; Lai, Weizheng; Yao, Li; Leong, Meng Fatt; Wan, Andrew C.A.; Zink, Daniele

doi:10.1016/j.biomaterials.2011.09.063

Cited by 11 publications

(21 citation statements)

References 44 publications

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“…2J). We showed before that unmodified glass is an excellent substrate for HPTC [14,20]. In contrast, only few cells were observed on HFM consisting of PES/PVP ( Fig.…”

Section: Hptc Performance On the Outer Surfaces Of Unmodified Commercmentioning

confidence: 77%

“…Furthermore, so far only human primary renal proximal tubular cells (HPTC) have been approved for clinical trials of BAK [4], and this cell type does not perform well on smooth and haemocompatible surfaces of synthetic membranes [10,[12][13][14][15]. To address this problem, protein coatings have been applied to the luminal surfaces of the HFM in BAK bioreactor units [1][2][3][4][5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

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A novel design of bioartificial kidneys with improved cell performance and haemocompatibility

Kandasamy

Tasnim

et al. 2013

J Cellular Molecular Medi

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Treatment with bioartificial kidneys had beneficial effects in animal experiments and improved survival of critically ill patients with acute kidney injury in a Phase II clinical trial. However, a Phase II b clinical trial failed. This and other results suggested various problems with the current design of bioartificial kidneys. We propose a novel design to improve various properties of device, including haemocompatibility and cell performance. An important feature of the novel design is confinement of the blood to the lumina of the hollow fibre membranes. This avoids exposure of the blood to the non-haemocompatible outer surfaces of hollow fibre membranes, which usually occurs in bioartificial kidneys. We use these outer surfaces as substrate for cell growth. Our results show that commercial hollow fibre membranes can be directly applied in the bioreactor when human primary renal proximal tubular cells are grown in this configuration, and no coatings are required for the formation of robust and functional renal epithelia. Furthermore, we demonstrate that the bioreactor unit produces significant amounts of interleukins. This result helps to understand the immunomodulatory effects of bioartificial kidneys, which have been observed previously. The novel bioartificial kidney design outlined here and the results obtained would be expected to improve the safety and performance of bioartificial kidneys and to contribute to a better understanding of their effects.

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“…2J). We showed before that unmodified glass is an excellent substrate for HPTC [14,20]. In contrast, only few cells were observed on HFM consisting of PES/PVP ( Fig.…”

Section: Hptc Performance On the Outer Surfaces Of Unmodified Commercmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

A novel design of bioartificial kidneys with improved cell performance and haemocompatibility

Kandasamy

Tasnim

et al. 2013

J Cellular Molecular Medi

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“…[1][2][3] It is also becoming apparent that tissue culture surfaces can selectively and specifi cally control the fate of individual cell populations. For example, the fate of mesenchymal stem cells can be regulated by controlling the density of the RGD cell adhesion ligand on the material surface.…”

Section: Introductionmentioning

confidence: 99%

Controlling the Dynamics of Cell Transition in Heterogeneous Cultures using Surface Chemistry

Hickman

Rees

Boocock

et al. 2014

Adv Healthcare Materials

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Developing materials that can preferentially select defined cancer cell populations for biological characterization will greatly enhance our understanding of cancer cell growth, differentiation, and invasion. The transitional events between epithelial and mesenchymal phenotypes are particularly crucial, as primary tumors and secondary metastasis are generally epithelial in nature, whereas circulating mesenchymal cells derived from primary epithelial cells appear to facilitate the spread of disease and its resistance to therapy. This study describes an amino-functionalized material, which promotes the enrichment of an epithelial phenotype from a single cell line containing both epithelial and mesenchymal subpopulations of cancer cells. The isolation and transitional control of such subpopulations using functional materials will advance understanding of the disease process, have a significant impact on the downstream development of new targeted cancer therapeutics, and also be applicable to tissue engineering and regenerative medicine.

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“…Surface characteristics of substrates have been substantially discussed in literatures for their role in regulating cell attachment, proliferation and even cell fate determination. For instance, topography [141][142][143] , pore size and roughness [60,[144][145][146][147] , chemical composition and functional groups [21,105,148,149] , and protein adsorption kinetics [144,145,[150][151][152] govern the interaction of living cells with surfaces. These properties could be evaluated qualitatively by microscopy, and quantitatively by following protein adsorption kinetics and by spectroscopy.…”

Section: Surface Propertiesmentioning

confidence: 99%

“…It enabled us to obtain insights about interaction of living cells with the two surfaces of ECM (top: decellularized pericardium; bottom: decellularized endocardium), in terms of amount of protein adsorbed. Bovine Serum Albumin labeled with fluorescein isothiocyanate (BSA-FITC) was used as a model protein for its well-characterized structure and physiochemical properties [146] ; and the assay used was modified from those of Ni et al [150] ; Koh et al [145] ; and Kim et al [151] with modifications to the concentration of solution, sample dimension and range of time points. replicas were taken for each sample in each group.…”

Section: Protein Adsorption Studymentioning

confidence: 99%

Biophysics of recellularized porcine cardiac extracellular matrix for myocardial tissue engineering

Au-Yeung¹

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The use of a library of industrial materials to determine the nature of substrate-dependent performance of primary adherent human cells

Cited by 11 publications

References 44 publications

A novel design of bioartificial kidneys with improved cell performance and haemocompatibility

A novel design of bioartificial kidneys with improved cell performance and haemocompatibility

Controlling the Dynamics of Cell Transition in Heterogeneous Cultures using Surface Chemistry

Biophysics of recellularized porcine cardiac extracellular matrix for myocardial tissue engineering

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