The use of a new ad hoc growth cell with parallel electrodes for the nucleation control of lysozyme

Moreno, Abel; Sazaki, Gen

doi:10.1016/j.jcrysgro.2003.12.071

Cited by 50 publications

(90 citation statements)

References 9 publications

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“…A growth technique mediated by screw dislocations under low supersaturation has also been proposed [29][30][31][32]. Techniques in which an electric field is applied to a protein solution have also been actively investigated, with a focus on controlling the nucleation rate [33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49].…”

Section: Introductionmentioning

confidence: 99%

Effect of an External Electric Field on the Kinetics of Dislocation-Free Growth of Tetragonal Hen Egg White Lysozyme Crystals

et al. 2017

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Dislocation-free tetragonal hen egg white (HEW) lysozyme crystals were grown from a seed crystal in a cell. The rates of tetragonal HEW lysozyme crystal growth normal to the (110) and (101) faces with and without a 1-MHz external electric field were measured. A decrease in the typical growth rates of the crystal measured under an applied field at 1 MHz was observed, although the overall driving force increased. Assuming that the birth and spread mechanism of two-dimensional nucleation occurs, an increase in the effective surface energy of the step ends was realized in the presence of the electric field, which led to an improvement in the crystal quality of the tetragonal HEW lysozyme crystals. This article also discusses the increase in the effective surface energy of the step ends with respect to the change in the entropy of the solid.

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Section: Introductionmentioning

confidence: 99%

Effect of an External Electric Field on the Kinetics of Dislocation-Free Growth of Tetragonal Hen Egg White Lysozyme Crystals

et al. 2017

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“…dc-electrochemically-assisted batch crystallization of lysozyme and ferritin has continued to be the focus of Moreno's interest while working (as a visiting professor) with Sazaki in his lab in Japan [7]. An EF (electric potential difference) that injects a dc between electrodes immersed in the solution (conventionally used in the following consideration as "internal EF"), has been first applied in the laboratory of Moreno [6].…”

Section: Crystallization In the Presence Of Electric Fields (Efs)mentioning

confidence: 99%

“…dc-electrochemically-assisted batch crystallization of lysozyme and ferritin has continued to be the focus of Moreno's interest while working (as a visiting professor) with Sazaki in his lab in Japan [7]. They have observed that applying a dc of 2 µA flowing between electrodes of platinum wires, the number of deposited lysozyme crystals significantly decreases, while the size and the quality of crystals increase [7].…”

Section: Crystallization In the Presence Of Electric Fields (Efs)mentioning

confidence: 99%

“…They have observed that applying a dc of 2 µA flowing between electrodes of platinum wires, the number of deposited lysozyme crystals significantly decreases, while the size and the quality of crystals increase [7]. Nucleation induction time for crystallization also decreases.…”

Section: Crystallization In the Presence Of Electric Fields (Efs)mentioning

confidence: 99%

“…Pioneered by Aubry's group [4,5] some 20 years ago, protein crystallization under EF attracts an increasing attention, becoming a mature scientific branch today. Major contributions in this research field have been made by the teams of Moreno [6][7][8][9][10][11][12][13][14][15][16][17], Koizumi [18][19][20][21][22][23][24][25][26][27][28][29], Veesler [30][31][32][33], etc. (the list is not exhaustive).…”

Section: Introductionmentioning

confidence: 99%

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Recent Insights into the Crystallization Process; Protein Crystal Nucleation and Growth Peculiarities; Processes in the Presence of Electric Fields

Nanev

2017

Crystals

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Three-dimensional protein molecule structures are essential for acquiring a deeper insight of the human genome, and for developing novel protein-based pharmaceuticals. X-ray diffraction studies of such structures require well-diffracting protein crystals. A set of external physical factors may promote and direct protein crystallization so that crystals obtained are useful for X-ray studies. Application of electric fields aids control over protein crystal size and diffraction quality. Protein crystal nucleation and growth in the presence of electric fields are reviewed. A notion of mesoscopic level of impact on the protein crystallization exercised by an electric field is also considered.Keywords: protein crystallization; classical and two-step nucleation mechanisms; impact of electric fields on the protein crystallization; external and internal electric fields; number density; size and quality of protein crystals

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Protein Crystal Growth Methods

Quezada

Arreguı́n-Espinosa

Moreno

2010

Springer Handbook of Crystal Growth

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The use of a new ad hoc growth cell with parallel electrodes for the nucleation control of lysozyme

Cited by 50 publications

References 9 publications

Effect of an External Electric Field on the Kinetics of Dislocation-Free Growth of Tetragonal Hen Egg White Lysozyme Crystals

Effect of an External Electric Field on the Kinetics of Dislocation-Free Growth of Tetragonal Hen Egg White Lysozyme Crystals

Recent Insights into the Crystallization Process; Protein Crystal Nucleation and Growth Peculiarities; Processes in the Presence of Electric Fields

Protein Crystal Growth Methods

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