The use of a plasma protein fraction in culture of human spleen leukocytes during induction of interferon

Coupin, G.; Benezra, Robert; Schmitthaüsler, R.; Illinger, D.; Oberling, F; Fauconnier, B; Lombard, Y.; Poindron, Philippe

doi:10.1016/s0769-2617(84)80043-x

Annales de l'Institut Pasteur / Virologie

1984

DOI: 10.1016/s0769-2617(84)80043-x

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The use of a plasma protein fraction in culture of human spleen leukocytes during induction of interferon

G. Coupin¹,

Robert Benezra²,

R. Schmitthaüsler

et al.

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1986

1992

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Cited by 2 publications

References 12 publications

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Mechanical Responses of Rat Vascular Smooth Muscle Cells to Rat Interferon

André¹,

Travo²,

Stoclet³

et al. 1986

Journal of Interferon Research

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Mechanical responses elicited by rat interferon (IFN) were investigated in rat vascular smooth muscle cells (RVSMC) in primary culture. IFN induced a dose-dependent reduction in the apparent cell surface area, which was quantitatively comparable to, or even greater than, the response elicited by the alpha-adrenergic agent, phenylephrine. Neither mock-IFN nor rat IFN-neutralized with specific antibodies elicited such a response. The sensitivity of RVSMC to the mechanical effect of IFN was species specific. In addition, the effect of IFN was inhibited by the Ca2+ entry blocker, verapamil.

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Mechanical Responses of Rat Vascular Smooth Muscle Cells to Rat Interferon

André¹,

Travo²,

Stoclet³

et al. 1986

Journal of Interferon Research

View full text Add to dashboard Cite

show abstract

Human Interferons-α Inhibit the Production of Immunoglobulin M by Secreting and Nonsecreting Lymphoblastoid Cell Lines

Hartmann¹,

Schatz²,

Wachsmann³

et al. 1992

Journal of Interferon Research

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It is well known that human lymphoblastoid interferon-alpha [HuIFN-alpha(ly)] may either increase or decrease antibody production by B cells, in vivo as well as in vitro, depending on the experimental conditions and the system used. We compare here the effect of HuIFN-alpha(ly) and human recombinant interferon-alpha (rHuIFN-alpha) on immunoglobulin M (IgM) production by secreting and nonsecreting human B-like lymphoblastoid cells, respectively, ST8246, and Daudi cells. Under our experimental conditions, Daudi cells were less sensitive to the antiproliferative effect of IFN than previously reported by other authors, and ST8246 cells were insensitive to this antiproliferative effect. In contrast, IgM production was profoundly affected in both cell lines. Thus, we could discriminate between the effect on cell growth from the effect on the immune response. Using high-pressure liquid chromatography (HPLC) separation, mu chains and monomeric and pentameric IgM were distinguished from cytosolic and membrane-associated fractions and from culture medium (extracellular IgM). Pentameric extracellular IgM and monomeric membrane IgM were diminished by HuIFN-alpha(ly) treatment, respectively, in ST8246 cells and in Daudi cells. We conclude that HuIFN-alpha(ly) induces regression of B-like lymphoblastoid cells toward a less mature phenotype.

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The use of a plasma protein fraction in culture of human spleen leukocytes during induction of interferon

Cited by 2 publications

References 12 publications

Mechanical Responses of Rat Vascular Smooth Muscle Cells to Rat Interferon

Mechanical Responses of Rat Vascular Smooth Muscle Cells to Rat Interferon

Human Interferons-α Inhibit the Production of Immunoglobulin M by Secreting and Nonsecreting Lymphoblastoid Cell Lines

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