2003
DOI: 10.1094/asbcj-61-0152
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The Use of Adenylate Kinase Measurement to Determine Causes of Lysis in Lager Yeast

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Cited by 6 publications
(7 citation statements)
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“…Partly because of the robust nature of the AK enzyme, its detection has been used to measure lysis of bacterial contaminants on food contact surfaces and autolysis of brewing yeast strains (18)(19)(20). Recently we showed that the AK assay is useful in the identification of antifungal agents (21,22).…”
Section: Rationale For Adenylate Kinase As a Reporter Of Bacterial Cementioning
confidence: 99%
“…Partly because of the robust nature of the AK enzyme, its detection has been used to measure lysis of bacterial contaminants on food contact surfaces and autolysis of brewing yeast strains (18)(19)(20). Recently we showed that the AK assay is useful in the identification of antifungal agents (21,22).…”
Section: Rationale For Adenylate Kinase As a Reporter Of Bacterial Cementioning
confidence: 99%
“…Therefore, we turned to AK release based on a report describing its use to detect lysis in brewing strains. 6 To test the feasibility of AK release as a reporter of yeast cell lysis, logarithmic phase S. cerevisiae BY4741 cells were incubated in YPD medium with 1% DMSO containing either 250 ng/mL CAS or no drug. CAS is a fungicidal antifungal that inhibits the synthesis of 1,3-β-glucan, a key component of the fungal cell wall.…”
Section: Ak Release As a Reporter Of Yeast Cell Lysismentioning
confidence: 99%
“…Extracellular AK activity has been used previously to detect autolysis of yeast strains in the brewing industry, but to our knowledge, it has not been applied to drug discovery. 6 As detailed below, the AK assay has a number of attractive features. First, it specifically detects fungicidal molecules.…”
Section: Introductionmentioning
confidence: 99%
“…A second type of viability assay that was recently developed is based on the detection of extracellular adenylate kinase (AK) as a reporter of cell lysis [62]. AK is a conserved enzyme that has been used as a cytotoxicity reporter in mammalian cell culture assays and as a reporter of brewing yeast autolysis in the beverage industry [63].…”
Section: Omics-based Antifungal Drug Discovery Approachesmentioning
confidence: 99%