The Use of Adipose Tissue–Derived Stem Cells within a Dermal Substitute Improves Skin Regeneration by Increasing Neoangiogenesis and Collagen Synthesis

Meruane, Manuel; Rojas, Mariana; Marcelain, Katherine

doi:10.1097/prs.0b013e3182547e04

Cited by 64 publications

(46 citation statements)

References 21 publications

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“…The observation window of the dorsal skinfold chamber allowed a continuous access to the implants and, T Später et al Vascularisation of Integra ® Young et al, 1998;Melendez et al, 2008;Danner et al, 2012;Meruane et al, 2012;Killat et al, 2013;Zonari et al, 2015). Recently, we could demonstrate that the seeding of dermal skin substitutes with ad-MVF, which already represent fully functional vessel segments, may be even more effective (Frueh et al, 2017).…”

Section: Epithelialisation Of the Implantsmentioning

confidence: 99%

“…Hence, the ad-MVF needed more time to bridge the distance between each other. In addition, the reassembly of ad-MVF into new microvascular networks has been shown to be crucially dependent on the interconnection of individual angiogenic sprouts growing out of the vessel segments (Nunes et al, 2010). In contrast to FWM, the extremely high density of ad-MVF on the surface of MWD may have markedly increased the probability for this event and the inosculation of the newly developing microvascular networks to the surrounding blood vessels of the host tissue.…”

Section: Epithelialisation Of the Implantsmentioning

confidence: 99%

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Potentials and limitations of Integra® flowable wound matrix seeded with adipose tissue-derived microvascular fragments

Später

Frueh

Menger

et al. 2017

eCM

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Adipose tissue-derived microvascular fragments (ad-MVF) represent promising vascularisation units for bioengineered Integra ® matrix wound dressing (MWD). However, due to the sheet-like structure with small pore sizes, the seeding of this matrix with ad-MVF is mainly limited to its surface. Integra ® flowable wound matrix (FWM) may be suitable to achieve a more homogeneous distribution and, thus, improved vascularisation, because this gel-like matrix allows for the direct admixture of ad-MVF during sample preparation. To test this hypothesis, we seeded MWD and FWM with an identical number of ad-MVF and assessed their distribution and inter-fragment distance within both matrices. Moreover, ad-MVF-seeded MWD and FWM were implanted into full-thickness skin defects within mouse dorsal skinfold chambers to analyse their vascularisation, epithelialisation and tissue incorporation using intravital fluorescence microscopy, histology and immunohistochemistry. Seeded FWM exhibited a more homogeneous ad-MVF distribution, when compared to MWD. This resulted in a significantly increased interfragment distance, preventing the reassembly of ad-MVF into new microvascular networks. Accordingly, the vascularisation of FWM was diminished after implantation, as indicated by a reduced functional microvessel density and blood perfusion. This was associated with a decreased tissue incorporation and epithelialisation of the matrix, when compared to ad-MVF-seeded MWD. Hence, the use of FWM as a carrier system may require a tremendous amount of ad-MVF to shorten their inter-fragment distance and, thus, to maintain their vascularisation capacity for tissue engineering applications.

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Section: Epithelialisation Of the Implantsmentioning

confidence: 99%

Section: Epithelialisation Of the Implantsmentioning

confidence: 99%

Potentials and limitations of Integra® flowable wound matrix seeded with adipose tissue-derived microvascular fragments

Später

Frueh

Menger

et al. 2017

eCM

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“…Estos resultados son consistentes con lo que se venía esbozando en nuestro estudio previo, que concluyó a la tercera semana de injerto (14). De este modo, presentamos una continuidad en cuanto a metodología, destacando que hemos mantenido un procedimiento simplificado de siembra de ASCs frescas, sin una expansión previa, durante un período de 48 horas.…”

Section: Discussionunclassified

“…En un estudio previo determinamos el porcentaje de adherencia y viabilidad celular mediante esta técnica (14).…”

Section: Siembra Celular Sobre La Matriz Dérmicaunclassified

“…Con anterioridad, presentamos una experiencia realizada en un modelo experimental en ratas en el que incorporamos ASCs en un segmento de Integra ® con una técnica simplificada, observando que a los 21 días de regeneración en vivo existe un aumento significativo de la angiogénesis en un 60% y de la síntesis de colágeno en un 55% respecto a un control; sin embargo, el aumento de la epitelización espontánea no fue significativo (14).…”

Section: Introductionunclassified

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Epitelización inducida por células troncales derivadas del tejido adiposo

Meruane

Benítez

Rojas

et al. 2014

Cir. plást. iberolatinoam.

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ResumenEl tratamiento de lesiones con pérdida de tejido cutáneo ha mejorado notablemente con el advenimiento de la bioingeniería tisular. Una alternativa en desarrollo es la utilización de sustitutos dérmicos combinados con células troncales derivadas del tejido adiposo autó-logo. Estudios previos nos muestran que con esta técnica es posible optimizar la angiogénesis y la síntesis de colágeno, sin embargo potenciar la epitelización es un tema pendiente por resolver. En el presente estudio evaluamos la progresión y diferenciación epitelial en un período de tiempo prologando.Obtuvimos las células troncales a partir del tejido adiposo (ASC) de la región inguinal de 4 ratas Sprague Dawley. Cultivamos las cé-lulas frescas en una matriz de Integra ® durante un período total de 48 horas, y las marcamos con un vector lentiviral-GFP (proteína fluorescente verde). Posteriormente, injertamos en las mismas ratas la matriz dérmica con células troncales y un implante contralateral sin células, como control. A las 4 semanas, evaluamos el avance epitelial mediante planimetría de superficie e histología.Los resultados macroscópicos muestran que el cierre de la herida por contracción de los bordes no tiene diferencias significativas (82,63%±3,4% vs. 80,66%±3,89%; p=0,08), pero el cierre por epitelización fue significativamente mayor en el lado intervenido con ASCs (93,47%±5,98% vs. 79,88%±6,28%; p=0,0028). Todas las muestras obtuvieron tinción positiva para el anticuerpo anti-citoqueratina 34βE12 y el avance epitelial lineal cuantificado por microscopía resultó significativamente mayor en el lado con ASCs (6408±275µm vs. 5375±250µm; p<0,001). Identificamos las células GFP positivas formando parte de la dermis regenerada, no así en la epidermis.En conclusión, las células troncales derivadas del tejido adiposo autólogo sembradas en una matriz de Integra® aumentan la formación epitelial significativamente, probablemente por un mecanismo de inducción más que de diferenciación. Palabras clave Epitelio epidérmico, Células madre,Tejido adiposo, Sustituto dérmico, Ingeniería tisular. Nivel de evidencia científica II AbstractThe treatment of injuries with loss of skin tissue has improved significantly with the advent of bioengineered tissue. Previous studies showed that the use of dermal substitutes combined with autologous Adipose-derived Stem Cells (ASCs) improve angiogenesis and collagen synthesis; however, epithelialization is an outstanding issue to be resolved. In the present study, we evaluated the epithelial progression and differentiation in an extended period of time.We obtained the adipose tissue derived stem cells (ASCs) from the inguinal fat pad of 4 Sprague Dawley rats. The non-expanded cells were cultivated in a dermal scaffold (Integra®) for 48 hours and marked with a green fluorescent protein (GFP) lentiviral vector. The scaffold plus stem cells and a contralateral cell-free scaffold (control) were implanted in the same rats. After 4 weeks, epithelial surface was assesses by planimetry and histology.The results show that ...

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Adipose tissue‐derived stem cells upon decellularized ovine small intestine submucosa for tissue regeneration: An optimization and comparison method

Khosroshahi

Rad

Kheirjou

et al. 2019

Journal Cellular Physiology

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The extracellular matrix of different mammalian tissues is commonly used as scaffolds in the field of tissue engineering. One of these tissues, which has frequently been studied due to its structural and biological features, is the small intestine submucosal membrane.These research are mainly done on the porcine small intestine. However, a report has recently been published about a scaffold produced from the submucosal layer of the ovine small intestine. In the present study, ovine small intestine submucosal (OSIS) was decellularized in a modified manner and its histological, morphological, and biomechanical properties were studied. Decellularization was performed in two phases: physical and chemical. In this method, a chloroform-methanol mixture, enzymatic digestion, and a constant dose of sodium dodecyl sulfate (SDS) was used in the least agitation time and its histological property and biocompatibility were evaluated in the presence of adipose tissue-derived stem cells (ADSCs); furthermore, ADSCs were isolated with a simple method (modified physical washing non-enzymatic isolation). The results were showed that the use of OSIS could be effective and operative. Mechanical properties, histological structure and shape, and glycosaminoglycan content were preserved. In the SDS-treated group, more than 90% of the native cells of tissue were deleted, and also in this group, no toxicity was observed and cell proliferation was supported, compared to the untreated group. Therefore, our results indicate that ADSCs seeded on OSIS scaffold could be used as a new approach in regenerative medicine as hybrid or hydrogel application.

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The Use of Adipose Tissue–Derived Stem Cells within a Dermal Substitute Improves Skin Regeneration by Increasing Neoangiogenesis and Collagen Synthesis

Cited by 64 publications

References 21 publications

Potentials and limitations of Integra® flowable wound matrix seeded with adipose tissue-derived microvascular fragments

Potentials and limitations of Integra® flowable wound matrix seeded with adipose tissue-derived microvascular fragments

Epitelización inducida por células troncales derivadas del tejido adiposo

Adipose tissue‐derived stem cells upon decellularized ovine small intestine submucosa for tissue regeneration: An optimization and comparison method

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