The use of AFLP markers for the identification of carrot breeding lines and F₁ hybrids

Abstract

Four inbred lines of carrot (cytoplasmic male‐steriles and corresponding maintainers) and eight of their F1 hybrids were studied with the amplified fragment length polymorphism (AFLP) technique to examine their genetic relationship and produce markers useful for testing hybrid seed purity. Eighty‐six polymorphic amplicons were identified in bulked DNA samples using eight primer pair combinations. Genetic distance was estimated on the basis of the presence or absence of polymorphic bands. The dendrogram plotted on the basis of the AFLP data closely represented the pedigree relationships of the lines and their hybrids. From one to six amplicons specific for a breeding line were identified. Most of them were also present in the DNA bulks of respective F1 hybrids. However, screening performed on individual plants of two parental lines and the corresponding hybrid indicated insufficient uniformity of parental lines, limiting the applicability of AFLP markers for testing hybrid seed purity.

DcMaster transposon display markers as a tool for diversity evaluation of carrot breeding materials and for hybrid seed purity testing

“…0 0 1 1 CMS = cytoplasmic male sterility fore breeding stocks used for the production of F 1 hybrids usually retain a relatively high level of heterozygosity, which was demonstrated in earlier inveatigations (Grzebelus et al 2001) and confirmed in the present study. Interestingly, a higher level of variability, similar to that of the open-pollinated cv.…”

“…The number of amplified products ranged from 9 (for the adaptor-specific primer carrying TCA as selective nucleotides) to 15 (ACT and GTA), and their length varied from 74 bp to 320 bp ( Table 2). The efficiency of DcMTD markers (9.3 polymorphic amplicons per primer combination) was only slightly lower than that obtained for AFLP markers (10.7 polymorphic amplicons per primer combination, Grzebelus et al 2001), and much higher than that obtained for RAPD markers (2.5 polymorphic amplicons per reaction, Grzebelus et al 1997) when similar plant materials were analysed.…”

“…Previous attempts towards developing such markers for carrot hybrids, using the AFLP marker system, were unsuccessful, owing to large intrapopulation variability (Grzebelus et al 2001). However, it is essential to assess the reliability of these markers through screening more plants from the populations in question.…”

“…This did not support conclusions from an earlier work by Shim and Jørgensen (2000), who stated that AFLP polymorphisms allowed varietal identification and corresponded with the geographic origin of wild D. carota populations. Intra-population diveristy was studied by using RAPD (Grzebelus et al 1997) and AFLP (Grzebelus et al 2001). Both marker systems revealed a high level of variability among the individuals from the same breeding stock.…”

Discrimination Between Celery Cultivars with the Use of Rapd Markers

“…The usefulness of DNA polymorphism based on randomly amplified polymorphic DNA (Welsh and McClelland, 1990;Williams et al, 1990;Caetano-Anoles et al, 1991) has been proved in many species of vegetable crops such as tomato (Archak et al, 2002;Singh et al, 2007), cabbage (Cansian and Echeverrigaray, 2000), cucumber (Horejsi and Staub, 1996), eggplant (Karihaloo et al, 1995), melon (Garcia et al, 1998), pepper (Votava and Bosland, 2001;(Rodriguez et al, 1999), and carrot (Grzebelus et al, 1997). While there are some limitations of this type of DNA marker such as the dominant character, unreliability, strict dependence on the conditions of PCR reaction, they can, however, be very useful for genetic study of populations of vegetable plants, their wild relatives, breeding lines and hybrids.…”