The Use of Agar Nutrient Solution to Simulate Lack of Convection in Waterlogged Soils

Hypoxia-Responsive Root Hydraulic Conductivity Influences Soybean Cultivar-Specific Waterlogging Tolerance

“…Some researchers have indicated that high carbon dioxide levels [30] [31] [32] or impaired mineral diffusion [33] in the rhizosphere are key factors influencing crop waterlogging injury. Additionally, low partial pressure of oxygen around the roots [17] [34] should be considered an important factor in the development of waterlogging injury in soybean plants.…”

Genotypic Variations in Responses of Lateral Root Development to Transient Moisture Stresses in Rice Cultivars

“…Oxygen deficiency was induced by mixing the water solution with agar (0.1% w/v) (referred herein as 'stagnant' treatment) (Wiengweera et al, 1997) and flushed with N 2 gas to reduce O 2 concentration in the water between 0.14 and 0.16 ppm. This solution was not aerated and remained 'stagnant' during the growing period.…”

“…This solution was not aerated and remained 'stagnant' during the growing period. Water culture with 0.1% agar prevents turbulence (convection), thus simulating the slow gas movement found in waterlogged soils (Wiengweera et al, 1997). On the other hand, drought was induced using polyethylene glycol 6000 (PEG 6000) (4%, W/V) to attain -0.13MPa water potential.…”

Utilizing Chromosome Segment Substitution Lines (CSSLs) for Evaluation of Root Responses to Transient Moisture Stresses in Rice

“…Oxygen deficiency was induced by mixing water solution with agar (0.1% w / v) (herein referred to as 'stagnant') (Wiengweera et al, 1997) and flushed with N 2 gas to reduce O 2 -concentration in the water from 8.0 ppm (well-aerated) to between 0.14 and 0.16 ppm. This agar-mixed water solution was not aerated and remained 'stagnant' during the growing period.…”

“…This agar-mixed water solution was not aerated and remained 'stagnant' during the growing period. Water culture with 0.1% agar prevents turbulence (convection), thus simulating the slow gas movement found in waterlogged soils (Wiengweera et al, 1997). On the other hand, drought was induced by adding polyethylene glycol 6000 (PEG 6000) (4%, w / v) to attain a -0.13MPa water potential.…”