The use of alizarin red S and alizarin complexone for immersion marking Japanese flounder Paralichthys olivaceus (T.)

Liu, Q.; Zhang, X.M.; Zhang, P.D.; Nwafili, Sylvanus Anene

doi:10.1016/j.fishres.2009.03.014

Cited by 47 publications

(58 citation statements)

References 25 publications

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“…Despite variations in the quality and lasting of the marks, fluorescent signals were detected in both treatments and all the structures analyzed. These findings are in agreement with several studies that showed ARS effectiveness for immersion marking, as well as its potential in biological research and evaluation of stock enhancement programs (Bashey, 2004;Baer & Rösch, 2008;Crook et al, 2007;Liu et al, 2009).…”

Section: Discussionsupporting

confidence: 92%

“…Similar observations were reported by Bashey (2004) in juvenile guppies Poecilia reticulata and by Crook et al (2007) in golden perch marking with ARS. None of the treatments have affected pejerrey growth and survival (Colautti et al, 2010), and these results are in agreement with data reported by Liu et al (2009) for the Japanese flounder.…”

Section: Discussionsupporting

confidence: 91%

“…The otoliths were all freed from adherent tissues and rinsed with water. All samples were observed directly without resin and polishing (Liu et al, 2009). After removed, pictures of the structures were taken in a darkened room using a fluorescence microscope (Nikon Eclipse E600-G-2E/C TRITC Filter) attached to a digital camera (Nikon Digital Sight DS-U2).…”

Section: Methodsmentioning

confidence: 99%

“…The most commonly used were oxytetracycline (OTC), hydrochloride, calcein, alizarin complexone (AC) and alizarin red S (ARS). However, calcein, ARS and AC performed better than other chemicals because: marks can be detected and read clearly, the effect over survival rates was proven to be less negative, and all can be used in salt water (Tsukamoto et al, 1989;Van der Walt & Faragher, 2003;Taylor et al, 2005;Baer & Rösch, 2008;Liu et al, 2009). Even though previously performed experiments using calcein have obtained clear and long-lasting marks, this chemical compound is expensive and was found to be toxic when fishes were exposed to it for long periods of time (Brooks et al, 1994;Bumguardner & King, 1996;Frenkel et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Osmotic induction marking with Alizarin Red S on juveniles of pejerrey, Odontesthes bonariensis (Atherinopsidae)

et al. 2013

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Juveniles of pejerrey, Odontesthes bonariensis, were exposed to 0.1% Alizarin Red S (ARS) alone or with a previous immersion in 2.2% saline solution (Osmotic Induction, OI) to enhance the ARS marking method. Fish were marked in the field and immediately released in 1 m 3 cages in "La Salada de Monasterio" lagoon, Chascomús, Buenos Aires, Argentina. After 73 days, clear marks were observed in the otoliths, caudal fin rays and scales with both treatments, being the intensity of the signal in the scales of OI+ARS treated fish higher. On the other hand, no marks were observed in the control group on the same structures. Approximately one year post-treatment (385 days), only marks in caudal fin rays were found clearly in OI+ARS treated fish. After this period, no significant differences in total length or weight between marked or control fish were observed and the mortality ranged between 30-40 % in all cages. These results provide strong evidence for the potential applicability of this cost-effective marking technique in differentiation of wild and hatchery-produced pejerrey. The success in the caudal fin rays marking is also important because it is easy to do and does not require the sacrifice of fish.Juvenis de pejerrey, Odontesthes bonariensis, foram expostos a Vermelho de Alizarina S (ARS) 0,1% de duas formas, sozinho ou com uma imersão anterior em 2,2% de solução salina (Indução osmótica, IO) para melhorar o método de marcação ARS. Os procedimentos foram realizados no campo e os peixes foram liberados em gaiolas (tanques-rede) de 1 m 3 na lagoa "La Salada de Monasterio", Chascomús, Buenos Aires, Argentina. Após 73 dias, marcas claras foram observadas nos otólitos, raios da nadadeira caudal e escamas em ambos os tratamentos, sendo que a intensidade do sinal nas escalas de IO + ARS de peixe tratado foi superior. Por outro lado, não foram observados marcas no grupo controle sobre as mesmas estruturas. Aproximadamente um ano pós-tratamento (385 dias), apenas marcas nos raios da nadadeira caudal foram encontrados claramente nos peixes tratados com IO+ARS. Entre os peixes observados, após este período, não houve diferenças significativas no comprimento total ou peso entre o grupo controle e marcados, ademais, a mortalidade variou entre 30-40% em todas as gaiolas. Estes resultados fornecem fortes evidências e um grande potencial para aplicação desta técnica rentável de marcação que diferencia o pejerrey selvagem e o produzido em cativeiro. O sucesso na marcação dos raios da nadadeira caudal também é de grande importância, pois sua verificação é fácil e não requer o sacrifício de peixes.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Osmotic induction marking with Alizarin Red S on juveniles of pejerrey, Odontesthes bonariensis (Atherinopsidae)

et al. 2013

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“…The presence of fluorescent marks may then be easily detected under UV light (Weber and Ridgway, 1962). In fishery practice, a few fluorochrome markers have been used for this procedure, such as tetracycline, oxytetracycline, tetracycline hydrochloride, calcein, alizarin red S, and alizarin complexone Brooks et al, 1994;Liu et al, 2009). Different methods of fluorochrome administration have been used for the mass marking of early life stages of fish.…”

Section: Introductionmentioning

confidence: 99%

Untitled

Stanczak¹,

Król²,

Żarski³

et al. 2017

Turk. J. Fish. Aquat. Sci.

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The aim of this study was to investigate the effectiveness of the per os marking method of otoliths in burbot at different early life stages. In experiment E1 burbot larvae 12 (DPH) and in E2 -22 (DPH) were fed with live Artemia salina nauplii immersed in alizarin red S (ARS) and tetracycline hydrochloride (TC), for 1, 3, or 6 subsequent days (in E1) and for 1, 4, or 8 subsequent days (E2). In experiment E3 the marking procedure involved feeding burbot juveniles (92 DPH) using a commercial feed supplemented with ARS at a doses of 0 to 100 g per kg of feed. In E1, the highest percentage of marked otoliths was recorded in the group fed for 6 days with A. salina immersed in TC. During E2, 100% of marked otoliths were recorded in the groups fed for 4 or 8 days, regardless of the type of dye used. The highest percentage of marked otoliths in E3 was noted in the group fed with 40 g ARS/kg feed. In conclusion, for mass marking of burbot using the per os method, feeding larvae at 22 DPH with ARS-immersed A. salina nauplii continuously for 4 days is recommended for fishery practice.

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Labeling Calcified Structures in Young Lake Sturgeon: A Comparison of Oxytetracycline Hydrochloride, Alizarin Red S, And Calcein

Withers

Markley

Davis

et al. 2019

N American J Fish Manag

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Unlike external marks and tags, fluorochrome markers provide researchers with the opportunity to examine structural changes and they act as reference points within the calcified structures that are used to assign ages to sampled fish. To compare the efficiency and retention of fluorochrome markers, captive age‐0 Lake Sturgeon, 118.49 mm TL (SD, 12.59), were exposed to four treatments: oxytetracycline hydrochloride (OTC; 0.07% for 8 h), calcein (0.5% for 4 min), alizarin red S (ARS; 0.5% for 4 min), or a control treatment. Each of these markers binds to calcium and they fluoresce yellow (OTC), green (calcein), or red (ARS) when excited by an ultraviolet (UV) light source. At 72 and 436 d following chemical exposure, the sagittal otoliths, leading pectoral fin spines, and dorsal scutes were harvested from the samples to look for the presence of the fluorochrome markers. The calcein and ARS marks were readily identified within all of the structures at both measurement points, but the OTC marks were less detectable, varying by structure and age. The application of relatively unused markers for mass‐marking and tracking changes in growth on calcified structures throughout Lake Sturgeons’ ontogeny shows promise and warrants further consideration and investigation.

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The use of alizarin red S and alizarin complexone for immersion marking Japanese flounder Paralichthys olivaceus (T.)

Cited by 47 publications

References 25 publications

Osmotic induction marking with Alizarin Red S on juveniles of pejerrey, Odontesthes bonariensis (Atherinopsidae)

Osmotic induction marking with Alizarin Red S on juveniles of pejerrey, Odontesthes bonariensis (Atherinopsidae)

Untitled

Labeling Calcified Structures in Young Lake Sturgeon: A Comparison of Oxytetracycline Hydrochloride, Alizarin Red S, And Calcein

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