The Use of Ampicillin in a Simplified Selective Medium for the Isolation of Fluorescent Pseudomonads

Simón, A.; Ridge, E.H.

doi:10.1111/j.1365-2672.1974.tb00464.x

Cited by 93 publications

(41 citation statements)

References 1 publication

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“…These growth conditions have been shown to be selective for fluorescent Pseudomonas spp. (Simon and Ridge, 1974). Fluorescent colonies that developed on these plates were isolated.…”

Section: Methodsmentioning

confidence: 99%

Antagonistic Potential of Fluorescent Pseudomonads and Control of Crown and Root Rot of Cucumber Caused by Phythophtora drechsleri

Shirzad¹,

Fallahzadeh-Mamaghani²,

Pazhouhandeh³

2012

The Plant Pathology Journal

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“…These growth conditions have been shown to be selective for fluorescent Pseudomonas spp. (Simon and Ridge, 1974). Fluorescent colonies that developed on these plates were isolated.…”

Section: Methodsmentioning

confidence: 99%

Antagonistic Potential of Fluorescent Pseudomonads and Control of Crown and Root Rot of Cucumber Caused by Phythophtora drechsleri

Shirzad¹,

Fallahzadeh-Mamaghani²,

Pazhouhandeh³

2012

The Plant Pathology Journal

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“…Pseudomonas strains (Table 1) were routinely cultured at 27°C in King's medium B (KMB) (19) or in the Pseudomonas selective medium KMB ϩϩϩ containing 40 g/ml ampicillin, 13 g/ml chloramphenicol, and 100 g/ml cycloheximide (39). All other bacterial genera were cultivated in LB broth (2) at 27°C, except Escherichia coli K-12, which was cultured at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Pyrroloquinoline Quinone Biosynthesis GenepqqC, a Novel Molecular Marker for Studying the Phylogeny and Diversity of Phosphate-Solubilizing Pseudomonads

Meyer

Frapolli

Keel

et al. 2011

Appl Environ Microbiol

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Many root-colonizing pseudomonads are able to promote plant growth by increasing phosphate availability in soil through solubilization of poorly soluble rock phosphates. The major mechanism of phosphate solubilization by pseudomonads is the secretion of gluconic acid, which requires the enzyme glucose dehydrogenase and its cofactor pyrroloquinoline quinone (PQQ). The main aim of this study was to evaluate whether a PQQ biosynthetic gene is suitable to study the phylogeny of phosphate-solubilizing pseudomonads. To this end, two new primers, which specifically amplify the pqqC gene of the Pseudomonas genus, were designed. pqqC fragments were amplified and sequenced from a Pseudomonas strain collection and from a natural wheat rhizosphere population using cultivation-dependent and cultivation-independent approaches. Phylogenetic trees based on pqqC sequences were compared to trees obtained with the two concatenated housekeeping genes rpoD and gyrB. For both pqqC and rpoD-gyrB, similar main phylogenetic clusters were found. However, in the pqqC but not in the rpoD-gyrB tree, the group of fluorescent pseudomonads producing the antifungal compounds 2,4-diacetylphloroglucinol and pyoluteorin was located outside the Pseudomonas fluorescens group. pqqC sequences from isolated pseudomonads were differently distributed among the identified phylogenetic groups than pqqC sequences derived from the cultivation-independent approach. Comparing pqqC phylogeny and phosphate solubilization activity, we identified one phylogenetic group with high solubilization activity. In summary, we demonstrate that the gene pqqC is a novel molecular marker that can be used complementary to housekeeping genes for studying the diversity and evolution of plant-beneficial pseudomonads.

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“…One series of five pots was kept uncultivated (bulk soil). Rhizosphere and bulk soil samples were obtained as described earlier (Raaijmakers et al, 1997) and dilution plated onto KMB agar supplemented (Simon and Ridge, 1974). After 48 h of incubation at 25 1C, colonies were counted and fluorescent pseudomonads were differentiated from non-fluorescent colonies under UV light (wavelength, 365 nm).…”

Section: Microorganisms and Soils Usedmentioning

confidence: 99%

Phenazine antibiotics produced by fluorescent pseudomonads contribute to natural soil suppressiveness to Fusarium wilt

et al. 2009

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Natural disease-suppressive soils provide an untapped resource for the discovery of novel beneficial microorganisms and traits. For most suppressive soils, however, the consortia of microorganisms and mechanisms involved in pathogen control are unknown. To date, soil suppressiveness to Fusarium wilt disease has been ascribed to carbon and iron competition between pathogenic Fusarium oxysporum and resident non-pathogenic F. oxysporum and fluorescent pseudomonads. In this study, the role of bacterial antibiosis in Fusarium wilt suppressiveness was assessed by comparing the densities, diversity and activity of fluorescent Pseudomonas species producing 2,4-diacetylphloroglucinol (DAPG) (phlD+) or phenazine (phzC+) antibiotics. The frequencies of phlD+ populations were similar in the suppressive and conducive soils but their genotypic diversity differed significantly. However, phlD genotypes from the two soils were equally effective in suppressing Fusarium wilt, either alone or in combination with non-pathogenic F. oxysporum strain Fo47. A mutant deficient in DAPG production provided a similar level of control as its parental strain, suggesting that this antibiotic does not play a major role. In contrast, phzC+ pseudomonads were only detected in the suppressive soil. Representative phzC+ isolates of five distinct genotypes did not suppress Fusarium wilt on their own, but acted synergistically in combination with strain Fo47. This increased level of disease suppression was ascribed to phenazine production as the phenazine-deficient mutant was not effective. These results suggest, for the first time, that redox-active phenazines produced by fluorescent pseudomonads contribute to the natural soil suppressiveness to Fusarium wilt disease and may act in synergy with carbon competition by resident non-pathogenic F. oxysporum.

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The Use of Ampicillin in a Simplified Selective Medium for the Isolation of Fluorescent Pseudomonads

Cited by 93 publications

References 1 publication

Antagonistic Potential of Fluorescent Pseudomonads and Control of Crown and Root Rot of Cucumber Caused by Phythophtora drechsleri

Antagonistic Potential of Fluorescent Pseudomonads and Control of Crown and Root Rot of Cucumber Caused by Phythophtora drechsleri

Pyrroloquinoline Quinone Biosynthesis GenepqqC, a Novel Molecular Marker for Studying the Phylogeny and Diversity of Phosphate-Solubilizing Pseudomonads

Phenazine antibiotics produced by fluorescent pseudomonads contribute to natural soil suppressiveness to Fusarium wilt

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