The Use of an Oral Fluid Immunoglobulin G ELISA for the Detection of <i>Helicobacter pylori</i> Infection in Children

Gilger, Mark A.; Tolia, Vasundhara; Johnson, Austin; Rabinowitz, Simon S.; Jibaly, Rima; Elitsur, Yoram; Chong, Sonny K. F.; Rosenberg, Allan J.; Gold, Benjamin D.; Rosenthal, Philip; Elkayam, O; Marchildon, P; Peacock, Jeffrey

doi:10.1046/j.1083-4389.2002.00062.x

Cited by 16 publications

(11 citation statements)

References 23 publications

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“…Of the 38 articles, 18 [52], [53], [56], [58], [59], [61], [62], [67], [69], [71], [73], [74], [76], [78], [79], [82], [85], [89] (47.5%) used culture and histological examination as the gold standard; ten [54], [60], [64], [70], [72], [80], [81], [83], [84], [87] (26.3%) used histology in combination with RUT and UBT; two articles [63], [68] (5.2%) used histology alone and seven articles [55], [57], [66], [75], [77], [86], [88] (18.4%) used UBT; and finally one article [65] (2.6%) used culture + UBT as gold-standard. For all articles the ELISA and WB tests did not form part of the reference standard.…”

Section: Resultsmentioning

confidence: 99%

“…Three papers in Spanish were included [62], [64], [76]. Thirty-one (81.5%) articles reported using a case-control design and the remaining seven [53], [57], [65], [66], [68], [69], [75] (18.5%) a cross-sectional design. Thirty-two of the 38 (84.2%) articles collected samples prospectively and six [53], [65], [66], [69], [74], [85] (15.8%) retrospectively; two [65], [75] (5.2%) articles reported the use of a randomly selected subset of the sample for validation with the reference standard.…”

Section: Resultsmentioning

confidence: 99%

“…Thirty-two of the 38 (84.2%) articles collected samples prospectively and six [53], [65], [66], [69], [74], [85] (15.8%) retrospectively; two [65], [75] (5.2%) articles reported the use of a randomly selected subset of the sample for validation with the reference standard. Eleven [52], [61], [63], [64], [67], [68], [78]–[81], [83] (28.9%) articles reported at least single-blinded interpretation of the ELISA and WB tests and reference standard results, while 27 (71.1%) articles did not mention the blinding status. Thirteen (34.2%) of the 38 articles reported evaluation of more than one diagnostic test against the gold-standard; in these cases each test comparison was counted as a separate study.…”

Section: Resultsmentioning

confidence: 99%

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Antibody-Based Detection Tests for the Diagnosis of Helicobacter pylori Infection in Children: A Meta-Analysis

et al. 2008

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BackgroundNumerous serologic tests are available for the diagnosis of H. pylori infection in children. Common designs of antibody-based detection tests are ELISA and Western Blot (WB). For developing countries with limited laboratory resources and access, ELISA would be the preferred method because of its simplicity, lower cost and speed. Although in adults ELISA has proven to be highly accurate in diagnosing H. pylori infection; in children, it has shown variable accuracy.Methods/FindingsWe conducted a systematic review and meta-analysis to assess the accuracy of antibody-based detection tests for the diagnosis of H. pylori infection in children. Selection criteria included participation of at least 30 children and the use of a gold standard for H. pylori diagnosis. In a comprehensive search we identified 68 studies. Subgroup analyses were carried out by technique, immunoglobulin class, and source of test (commercial and in-house). The results demonstrated: 1) WB tests showed high overall performance, sensitivity 91.3% (95% CI, 88.9–93.3), specificity 89% (95% CI, 85.7–91.9), LR+ 8.2 (95% CI, 5.1–13.3), LR− 0.06 (95% CI, 0.02–0.16), DOR 158.8 (95% CI, 57.8–435.8); 2) ELISA-IgG assays showed low sensitivity 79.2% (95% CI, 77.3–81.0) and high specificity (92.4%, 95% CI, 91.6–93.3); 3) ELISA commercial tests varied widely in performance (test for heterogeneity p<0.0001); and 4) In-house ELISA with whole-cell antigen tests showed the highest overall performance: sensitivity 94% (95% CI, 90.2–96.7), specificity 96.4% (95% CI, 94.2–97.9), LR+ 19.9 (95% CI, 7.9–49.8), LR− 0.08 (95% CI, 0.04–0.15) DOR 292.8 (95% CI, 101.8–841.7).Conclusions/SignificanceWB test and in-house ELISA with whole-cell antigen tests are the most reliable tests for the diagnosis of H. pylori infection in children. Antigens obtained from local strains of the community could partially explain the good overall accuracy of the in-house ELISA. Because of its cost and technical demands, in-house ELISA might be more suitable for use in developing countries.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Antibody-Based Detection Tests for the Diagnosis of Helicobacter pylori Infection in Children: A Meta-Analysis

et al. 2008

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“…Antibody tests (ELISA) to detect Hp infection in various body fluids were the first to appear, but they have failed to provide adequate accuracy to replace gastric biopsy as the gold standard, especially in young children. 4,[7][8][9] The 13 C urea breath test (UBT) was developed to detect Hp organisms by measuring the radiolabel carbon in the breath of infected children. Studies from Europe and Canada reported a very good accuracy rate, [10][11][12] and expert guidelines have suggested this method for monitoring bacterial eradication after therapy.…”

Section: Helicobacter Pylori Diagnostic Tools: Is It In the Stool?mentioning

confidence: 99%

Helicobacter pylori diagnostic tools: Is it in the stool?

Elitsur

2005

The Journal of Pediatrics

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“…As blood sampling is not needed, these tests are extremely noninvasive and thus, their possible use especially in small children is of interest. However, also in recent papers, these tests have shown disappointingly low sensitivities [68,69]. In a Chinese population, a rapid urine test was highly accurate (sensitivity 96.7%, specificity 95.2%) as compared to invasive tests [70].…”

Section: Serological Testsmentioning

confidence: 99%

Diagnosis of Helicobacter pylori infection

López‐Brea¹,

Alarcón²,

Mégraud³

2003

Helicobacter

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Numerous studies are still published on diagnostic tests for Helicobacter pylori, essentially for noninvasive tests. The urea breath test is applied in different types of patients, and there are attempts to have quicker and simpler protocols. Stool tests using monoclonal antibodies are now evaluated while serology is still a subject of interest. The progress in PCR (multiplex PCR, real-time PCR) has also stimulated the research in this area of invasive tests, in order to get insight into virulence factors, macrolide susceptibility and to detect H. pylori in nonconventional specimens.

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The Use of an Oral Fluid Immunoglobulin G ELISA for the Detection of Helicobacter pylori Infection in Children

Abstract: Overall, we conclude that this oral fluid ELISA does not appear to be a helpful clinical tool for the diagnosis of H. pylori infection in children.

Cited by 16 publications

References 23 publications

Antibody-Based Detection Tests for the Diagnosis of Helicobacter pylori Infection in Children: A Meta-Analysis

Antibody-Based Detection Tests for the Diagnosis of Helicobacter pylori Infection in Children: A Meta-Analysis

Helicobacter pylori diagnostic tools: Is it in the stool?

Diagnosis of Helicobacter pylori infection

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