The Use of Angelica Sinensis and ß-Tricalcium Phosphate in the Management of Surgically Induced Critical-sized Class Ii Furcation Defects in Dogs

Abstract

Objective: Evaluating the effectiveness of Angelica sinensis, a widely used traditional Chinese medicine, in conjunction with Beta-tricalcium phosphate (β-TCP) bone graft in the management of induced critical sized class II furcation defects in dogs.Materials and Methods: A randomized study design was conducted on the third and fourth premolars of six healthy mongrel dogs. A total of twenty-four class II furcation critical-sized defects were surgically created. After reflecting a fully-reflected mucoperiosteal flap, twelve defects were filled with Angelica sinensis powder mixed with β-TCP, then collagen membranes were applied over the defects. The other twelve defects were filled with β-TCP followed by collagen membrane application. The dogs were sacrificed at the end of fourth and eighth weeks. Jaw segments teeth were dissected and processed for histological examination and morphometric analysis of the regenerative features.Results: The histological results revealed that bone formation and regeneration of cementum and PDL progressed more in experimental groups than in control groups. Moreover, the regenerative features were more advanced in the second observation period. The histomorphometry revealed that the height of newly formed interradicular bone, its surface area, and the thickness of its trabeculae were higher in the defects treated with Angelica sinensis and β-TCP than in only β-TCP-filled defects at both observational periods.Conclusions: Angelica sinensis has accelerated the regenerative potential of the interradicular bone and has enhanced the effect of β-TCP.Clinical Significance: Since Angelica sinensis herb has resulted in a higher periodontal regeneration as proved histologically, its conjunction with bone grafts at furcation treatments is clinically recommended.