The Use of Antibiotics as Chiral Selectors in Capillary Electrophoresis: A Review

Abstract

Chirality is becoming an essential issue in modern pharmaceutical research as regulatory agencies emphasize the safety and efficiency of enantiomers in drug development. The development of efficient and reliable chiral separation methods became a necessity in the last 30 years, and capillary electrophoresis (CE), due to its relatively low costs and “green” features, is attracting increased attention. Cyclodextrin (CD) and their derivatives are the most frequently used chiral selectors (CSs) in CE, however, the use of antibiotics as CSs represents an interesting alternative. Various classes of antibiotics (aminoglycosides, ansamycins, glycopeptides, lincosamides, macrolides, tetracyclines) have been used more or less successfully for the enantio-separation of pharmaceuticals. Antibiotics offer the possibility of a multitude of potential interactions (electrostatic, inclusion, hydrogen bonding, etc.) due to their chemical diversity, allowing the enantio-separation of analytes with a wide range of structural characteristics. This article aims to review the application of various classes of antibiotics in the CE enantio-separation of pharmaceuticals. Antibiotic physiochemical characteristics, variables impacting enantio-separation, advantages, and disadvantages when certain antibiotics are used as CSs in CE are also explored.

Capillary Electrophoresis: A Three-Year Literature Review

“…In addition to CDs and their derivatives, other chiral selectors were also applied to enantioseparations such as polysaccharides, proteins, and crown ethers over the past few years. − Antibiotics are another type of chiral selectors that have been widely used in CE . Novel antibiotic-type chiral selectors reported in the past couple of years include gamithromycin, fusidic acid, and kasugamycin .…”

“…58−60 Antibiotics are another type of chiral selectors that have been widely used in CE. 61 Novel antibiotic-type chiral selectors reported in the past couple of years include gamithromycin, 62 fusidic acid, 63 and kasugamycin. 64 Each of these chiral selectors has its own unique properties and is suitable for the separation of specific chiral compounds.…”

Investigation on improvement of enantioseparation based on clindamycin phosphate by chiral deep eutectic solvents in capillary electrophoresis

“…Capillary electrophoresis (CE) is an effective technology for enantioseparation owing to its unique advantages [3]. Various compounds have been successfully developed as chiral selectors in CE over the past few decades, including cyclodextrins and their derivatives [4], saccharides [5], antibiotics [6], proteins [7], crown ethers [8], and so on. Despite all this, the chiral recognition ability of many chiral selectors is limited.…”

The Enantioselective Potential of NicoShell and TeicoShell Columns for Basic Pharmaceuticals and Forensic Drugs in Sub/Supercritical Fluid Chromatography

“…Macrocyclic glycopeptides (MGs) are broadly utilized as chiral selectors in liquid chromatography (LC) [ 1 ] and capillary electrophoresis [ 2 ] and are successfully employed in sub/supercritical fluid chromatography (SFC) [ 3 , 4 ]. Their complex structure and multiple functional groups allow enantiorecognition of a broad range of compounds [ 5 ].…”