The use of asymmetric-field inversion gel electrophoresis to predict tumor cell radiosensitivity

Giaccia, Amato J.; Schwartz, Jeffrey L.; Shieh, Jennifer C.; Brown, J. Martin

doi:10.1016/0167-8140(92)90229-n

Cited by 42 publications

(12 citation statements)

References 30 publications

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“…Cell lines with a high number of dsb induced found to be much more sensitive than cell lines with a low number of induced dsb. This was similarly found by Ruiz de Almodovar et al (McMillan et al, 1990;Ruiz de Almodovar et al, 1994;Whitaker et al, 1995), other studies showed an insignificant trend (Schwartz et al, 1988;Giaccia et al, 1992;Zaffaroni et al, 1994;McKay and Kefford, 1995;Woudstra et al, 1998). In our study, one (LNCaP, Figure 7A) out of the nine cell lines fell off the general relationship between initial damage and cell survival indicating that the cellular radiosensitivity is eventually not only determined by the number of induced dsb but also by other still unknown factors.…”

Section: Relationship Between Induced Damage and Cellular Radiosensitsupporting

confidence: 74%

“…Some authors did not mention differences in particular; however, variations were in the same order of magnitude as the above reports (Giaccia et al, 1992;Olive et al, 1994;McKay and Kefford, 1995). There was only one report (Olive et al, 1994) that found differences among six tumour cell lines using neutral filter elution but factually not by PFGE.…”

Section: Relationship Between Induced Damage and Cellular Radiosensitmentioning

confidence: 88%

“…11 out of 13 studies reported on a variation in the number of induced dsb (Kelland et al, 1988;Schwartz et al, 1988Schwartz et al, , 1990Schwartz et al, , 1991McMillan et al, 1990;Giaccia et al, 1992;Olive et al, 1994;Ruiz de Almodovar et al, 1994;Zaffaroni et al, 1994;McKay and Kefford, 1995;Whitaker et al, 1995;Woudstra et al, 1998;Eastham et al, 2001), but only three found a correlation with cell killing (McMillan et al, 1990;Ruiz de Almodovar et al, 1994;Whitaker et al, 1995). In repair studies, a correlation between cell killing and the number of dsb was only found after short repair intervals (up to 2 h) (Schwartz et al, 1988(Schwartz et al, , 1990Giaccia et al, 1992;Zaffaroni et al, 1994), but never for nonreparable dsb.…”

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confidence: 99%

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Radiosensitivity of human tumour cells is correlated with the induction but not with the repair of DNA double-strand breaks

2003

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Nine human tumour cell lines (four mammary, one bladder, two prostate, one cervical, and one squamous cell carcinoma) were studied as to whether cellular radiosensitivity is related to the number of initial or residual double-strand breaks (dsb). Cellular sensitivity was measured by colony assay and dsb by means of constant-and graded-field gel electrophoresis (CFGE and GFGE, respectively). The nine tumour cell lines showed a broad variation in cellular sensitivity (SF2 0.17 -0.63). The number of initial dsb as measured by GFGE ranged between 14 and 27 dsb/Gy/diploid DNA content. In contrast, normal fibroblasts raised from skin biopsies of seven individuals showed only a marginal variation with 18 -20 dsb/Gy/diploid DNA content. For eight of the nine tumour cell lines, there was a significant correlation between the number of initial dsb and the cellular radiosensitivity. The tumour cells showed a broad variation in the amount of dsb measured 24 h after irradiation by CFGE, which, however, was not correlated with the cellular sensitivity. This residual damage was found to be influenced not only by the actual number of residual dsb, but also by apoptosis and cell cycle progression which had impact on CFGE measurements. Some cell line strains were able to proliferate even after exposure to 150 Gy while others were found to degrade their DNA. Our results suggest that for tumour cells, in contrast to normal cells, the variation in sensitivity is mainly determined by differences in the initial number of dsb induced.

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Section: Relationship Between Induced Damage and Cellular Radiosensitsupporting

confidence: 74%

Section: Relationship Between Induced Damage and Cellular Radiosensitmentioning

confidence: 88%

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confidence: 99%

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Radiosensitivity of human tumour cells is correlated with the induction but not with the repair of DNA double-strand breaks

2003

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“…However, in our work, there were no significant differences in the slopes of the residual DNA dsb dose-response curves fitted by linear regression and SF2 after HDR and LDR irradiation. Nevertheless other human tumour studies measuring DNA dsbs by asymmetric field-inversion gel electrophoresis (Giaccia et al, 1992) and neutral filter elution (Zaffaroni et al, 1994) have indeed shown significant correlations between the level of residual dsbs and radiosensitivity.…”

Section: Discussionmentioning

confidence: 96%

“…A number of reports indicate that tumour cell radiosensitivity is positively correlated with the levels of initial radiation-induced DNA dsbs (Kelland et al, 1988;Peacock et al, 1989;McMillan et al, 1990;Ruiz de Almodovar et al, 1994), while others have shown no correlation (Smeets et al, 1993;Olive et al, 1994;McKay and Kefford, 1995). Positive correlations between tumour cell radiosensitivity and the extent of residual DNA dsbs (Giaccia et al, 1992;Zaffaroni et al, 1994), the rate of DNA dsb repair (Schwartz et al, 1988) and the misrepair of radiation-induced DNA damage (Powell et al, 1992;Powell and McMillan, 1994) have also been reported.…”

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The ratio of initial/residual DNA damage predicts intrinsic radiosensitivity in seven cervix carcinoma cell lines

Marples

Longhurst²,

Eastham³

et al. 1998

Br J Cancer

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Summary The single-cell gel electrophoresis (comet) assay was used to measure radiation-produced DNA double-strand breaks (dsbs) in a series of seven cervical tumour cell lines (ME180, HT3, C33A, C41, SiHa, MS751 and CaSki). The proportion of DNA dsbs was measured immediately after radiation treatment (initial damage) and 16 h later after incubation at 370C (residual damage). Linear dose-response curves were seen for initial (slopes 0.23-0.66) and residual (slopes 0.16-0.87) DNA dsbs. Neither of the slopes of the linear regression analysis on the initial and on the residual DNA dsbs dose-response curves (range 0-80 Gy) correlated with SF2 (surviving fraction at 2 Gy) measured after high-(HDR) or low-dose-rate (LDR) irradiation. An association was evident between SF2 after HDR and LDR irradiation and the ratio of the absolute level of initial and residual damage after a single dose of 60 Gy. However, a significant correlation was found between HDR (r= -0.78, P = 0.04) and LDR (r = -0.86, P = 0.03) SF2 values and the ratio of the slopes of the initial and residual DNA dsbs dose-response curves (range 0.47-0.99), representing the fraction of DNA damage remaining. These results indicate that the neutral comet assay can be used to predict radiosensitivity of cervical tumour cell lines by assessing the ratio of initial and residual DNA dsbs.

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The effect of radiation on G2 blocks, cyclin B expression and cdc2 expression in human squamous carcinoma cell lines with different radiosensitivities

Smeets

Mooren

Begg

1994

Radiotherapy and Oncology

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The use of asymmetric-field inversion gel electrophoresis to predict tumor cell radiosensitivity

Cited by 42 publications

References 30 publications

Radiosensitivity of human tumour cells is correlated with the induction but not with the repair of DNA double-strand breaks

Radiosensitivity of human tumour cells is correlated with the induction but not with the repair of DNA double-strand breaks

The ratio of initial/residual DNA damage predicts intrinsic radiosensitivity in seven cervix carcinoma cell lines

The effect of radiation on G2 blocks, cyclin B expression and cdc2 expression in human squamous carcinoma cell lines with different radiosensitivities

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