The use of autologous platelet gel to treat difficult‐to‐heal wounds: a pilot study

Mazzucco, Laura; Medici, D; Serra, Massimo; Panizza, Renzo; Rivara, Giorgio; Orecchia, Sara; Libener, Roberta; Cattana, Elena; Levis, Alessandro; Betta, Pier Giacomo; Borzini, P

doi:10.1111/j.1537-2995.2004.03366.x

Cited by 200 publications

(185 citation statements)

References 30 publications

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“…Among all, the issue here is about the effect of PRP on neural tissue and the concern of its effectiveness in the management of peripheral neuropathies and the relief of NP. Studies arguing in favor of the use of PRP in neuropathies and/or NP, consider that PRP or stem cells can exert an anti-inflammatory effect through cytokine release, and consider the role of PRP or stem cells in nerve healing and regeneration, combating thus the pathological inflammation involved in neuropathic pain (53)(54)(55)(56)(57). This …”

Section: Current Schemes Treatments For Np and Our Cell-based Proposalmentioning

confidence: 99%

Platelet-rich plasma in treating peripheral neuropathic pain. Preliminary report

Lopez¹,

Cortes²,

García-Ceballos³

et al. 2017

Rev. Soc. Esp. Dolor.

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Section: Current Schemes Treatments For Np and Our Cell-based Proposalmentioning

confidence: 99%

Platelet-rich plasma in treating peripheral neuropathic pain. Preliminary report

Lopez¹,

Cortes²,

García-Ceballos³

et al. 2017

Rev. Soc. Esp. Dolor.

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“…Platelets are easily collected from the blood stream and are concentrated in a small volume of plasma known as platelet-rich plasma (PRP). PRP has been used in a variety of surgical settings for soft-tissue healing enhancement, including chronic lower extremity ulcer healing (Mazzucco et al, 2004;O'Connell et al, 2008), and tendon healing (Franchini et al, 2006;Gamradt et al, 2007;Maniscalco et al, 2008;Sánchez et al, 2007;Virchenko and Aspenberg, 2006). It is also used in orthopaedic and oral maxillofacial surgery where it accelerates autogenous bone graft healing (Fennis et al, 2004;Marx et al, 1998;Simon et al, 2009), and in cosmetic surgery (Man et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

A recently developed bifacial platelet-rich fibrin matrix

Lucarelli

Beretta

Dozza

et al. 2010

eCM

120

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Platelet-rich plasma (PRP) is used clinically in liquid or gel form to promote tissue repair. Because of the poor mechanical properties, conventional PRP is often difficult to handle when used in clinical settings and requires secure implantation in a specific site, otherwise when released growth factors could be washed out during an operation. In this study, we analyzed the end product of a recently developed commercially available system (FIBRINET ® ), which is a dense pliable, platelet-rich fibrin matrix (PRFM). We characterized the mechanical properties of PRFM and tested whether PRFM releases growth factors and whether released factors induce the proliferation of mesenchymal stem cells (MSC). Mechanical properties as well as platelet distribution were evaluated in PRFM. PRFM demonstrated robust mechanical properties, with a tear elastic modulus of 937.3 + 314.6 kPa, stress at a break of 1476.0 + 526.3 kPa, and an elongation at break of 146.3 + 33.8 %. PRFM maintained its mechanical properties throughout the testing process. Microscopic observations showed that the platelets were localized on one side of the matrix. Elevated levels of PDGF-AA, PDGF-AB, EGF, VEGF, bFGF and TGF-β1 were measured in the day 1-conditioned media (CM) of PRFM and growth factor levels decreased thereafter. BMP2 and BMP7 were not detectable. MSC culture media supplemented with 20% PRFM-CM stimulated MSC cell proliferation; at 24 and 48 hours the induction of the proliferation was significantly greater than the induction obtained with media supplemented with 20% foetal bovine serum. The present study shows that the production of a dense, physically robust PRFM made through high-speed centrifugation of intact platelets and fibrin in the absence of exogenous thrombin yields a potential tool for accelerating tissue repair.

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“…It is currently understood that platelets also play a pivotal role in the repair of injured tissues since they contain platelet growth factors that is released from activated platelets; platelet growth factors initiate and modulate wound healing in bone or soft tissue (Bourquie et al 1993, Mazzuco et al 2004. Of particular importance in these healing processes is platelet-derived growth factor (PDGF), which exists as both heterodimers of A and B chains and as homodimers of either A -A or B -B chains (Pierce et al1991).…”

mentioning

confidence: 99%

Platelet-rich plasma preparation using three devices: Implications for platelet activation and platelet growth factor release

et al. 2006

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The use of autologous platelet gel to treat difficult‐to‐heal wounds: a pilot study

Abstract: Patients with chronic unhealing wounds showed substantial improvement when treated with PLT gel lesion dressings.

Cited by 200 publications

References 30 publications

Platelet-rich plasma in treating peripheral neuropathic pain. Preliminary report

Platelet-rich plasma in treating peripheral neuropathic pain. Preliminary report

A recently developed bifacial platelet-rich fibrin matrix

Platelet-rich plasma preparation using three devices: Implications for platelet activation and platelet growth factor release

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