The use of basic Fuchsin in Plant Anatomy

Camp, W. H.; Liming, Franklin G.

doi:10.3109/10520293209116080

Cited by 4 publications

(2 citation statements)

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“…To study plant structure, standard methods include dye tracing, microscopy techniques, X-ray tomography [ 17 , 18 ], neutron radiography [ 19 – 21 ], air-coupled ultrasound [ 22 ], laser-based guided waves [ 23 ], and 2D-light transmission [ 24 , 25 ]. Dye tracing has been used to study plant structure for almost a century [ 26 ], and functions by staining certain anatomical structures, and the distribution of dyes can be observed either macroscopically or under the microscope. Microscopy techniques are also well established and can be used to study microstructures within a certain depth.…”

Section: Introductionmentioning

confidence: 99%

Towards portable MRI in the plant sciences

Blystone,

Nuixe,

Traoré

et al. 2024

Plant Methods

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Plant physiology and structure are constantly changing according to internal and external factors. The study of plant water dynamics can give information on these changes, as they are linked to numerous plant functions. Currently, most of the methods used to study plant water dynamics are either invasive, destructive, or not easily accessible. Portable magnetic resonance imaging (MRI) is a field undergoing rapid expansion and which presents substantial advantages in the plant sciences. MRI permits the non-invasive study of plant water content, flow, structure, stress response, and other physiological processes, as a multitude of information can be obtained using the method, and portable devices make it possible to take these measurements in situ, in a plant’s natural environment. In this work, we review the use of such devices applied to plants in climate chambers, greenhouses or in their natural environments. We also compare the use of portable MRI to other methods to obtain the same information and outline its advantages and disadvantages.

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Section: Introductionmentioning

confidence: 99%

Towards portable MRI in the plant sciences

Blystone,

Nuixe,

Traoré

et al. 2024

Plant Methods

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“…Stem clearings were prepared by the method of Camp and Liming (1932). Clearings of hypocotyls with attached stem and primary root base were made by Jacob's (1952) technique.…”

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confidence: 99%

Ontogeny and Correlative Relationships of the Primary Thickening Meristem in Four‐o'clock Plants (Nyctaginaceae) Maintained Under Long and Short Photoperiods

Mikesell

Popham

1976

American J of Botany

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The developmental anatomy of Mirabilis jalapa was investigated during the first 90 days of growth. The primary thickening meristem (PTM) initially differentiates in the pericycle at the top of the cotyledonary node 18 days after germination, then basipetally in the pericycle through the hypocotyl. The PTM differentiates acropetally into the stem and in the pericycle of the primaiy root, commencing 22 days after germination. Endodermis is easily identifiable in hypocotyls as well as in primary roots because of Casparian thickenings in its cells. It has not been definitely identified in stems. There are three rings of primary vascular bundles in the stem. The PTM differentiates as segments of cambium in a layer of cells (probably in the pericycle) on an arc between vascular bundles of the outer bundle ring. Later, arcs of PTM differentiate externally to the phloem of each bundle. Each arc forms a connection between original segments of PTM lying on either side of each vascular bundle. Thus, the PTM becomes a continuous cylinder. The PTM differentiates in the pericycle outside vascular tissue in the hypocotyl and root. Differentiation of the PTM and the mode of secondary thickening is similar in plants exposed to short (8‐hr) and to long (18‐hr) photoperiods, but some differences were observed. The PTM differentiates closer to the stem apex in all plants over 18 clays of age growing vegetatively under long photoperiods. That is, the diffuse lateral meristem, in whose cells the PTM differentiates in young intemodes, is shorter in nearly all investigated plants growing in long photoperiods. The hypocotyl and base of the primary root of 40‐day‐old plants in short photoperiods were more enlarged than those of the same age plants in long photoperiods; but, at the end of 64 days, the hypocotyl and primaiy root base were larger in plants growing under short photoperiods. Thirty‐four days after seed germination, flower initiation occurs in plants exposed to short photoperiods. One hundred fifty days after seed germination, flowers differentiate on plants exposed to long photoperiods.

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Untersuchungen zur biologischen Aktivität der Phenylborsäure

Haccius

Massfeller

1961

Planta

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Wie bereits klirz mitgeteilt (HAcclus 1959(HAcclus und 1960 HAcclus und MASS~ELLE~ 1959), haben wir an Embryonen und Vegetationspunkten verschiedener Pflanzen-Arten beobaehtet, dab Phenylborsiiure (PhB) die Ausdifferenzierung von Blattprimordien w~hrend einer eng begrenzten und sieher sehr frfihen Entwicklungsphase total zu hemmen vermag, wi~hrend benaehbarte etwas weiter fortgesehrittene Anlagen unbeein-flul~t bleiben.Wir haben diesen Effekt inzwisehen n~ther untersueht. Im folgenden wird fiber Versuehe mit Jungpflanzen yon Kalanchoe bloss/eldiana und Cucumis sativus beriehtet und die Ergebnisse diskutiert. I. Material und Methoden1. Samen yon Kalanchoe blos.sfeldiana (Sorte ,,Leuchtstern ~ und ,,Richard Gr~iser") werden im Gew~chshaus zum Keimen gebracht, pikiert und die jungen Pflanzen unter Kurztagbedingungen gehMten. Iqach EntfMtung des dritten bis ffinften ~uf die Kotyledonen fo]genden Blattpaares beginnen wir mit der Phenyl-bors~ure-Applikation. Mit Hilfe einer 1 ml-Rekordspritze (Teilung 0,01 ml) werden je 0,03 ml einer 250 ppm-LSsung (~ 2.10 -3 m) auf die Endknospe des Hauptsprosses aufgebracht. Der Tropfen ist so bemessen, da~ er yon den jungen Bl~ttchen fest-gehMten wird und die Sprol~spitze v611ig umgibt. Eine zehnmMige Applikation der L6sung in dreit~tgigen Abst~nden erwies sich bei Kalanchoe am wirksamsten.2. Im Gew~ehsh~us gekeimte S~mlinge yon Cucumis sativus, Sorte ,,Vorgebirgs-Tr~uben", werden in 15 em-T(ipfe ausgepflanzt. Die Beh~ndlung erfolgt nach der fiir K. blossfeldiana gesehilderten Methode: Ein Tropfen (0,03 ml) der PhB-LSsung (250 ppm) wird auf die Endl~lospe aufgebraeht. Dabei erwies sich, im Gegensatz zu Kalanchoe, eine einmalige Applikation als ausreichend zur Er.. zielung des Effektes. Da, wie im folgenden n~iher uusgefiihrt, die PhB-Wirkung durch den Entwicklungszustand der zu behandelnden PIl~inzchen entscheidend beeinfluBt wird, wahlen wir fiir den Versuch nieht nur gleich alte, sondern auch ~uBerlich mSglichst iibereinstimmende Pflanzen aus.3. Bei der verwendeten Phenylborsaure handelte es sich bei der Mehrzahl der Versuehe um ein k~ufliehes Pr~parat der Firm~ Heyl & Co. (Berlin-Steglitz). Aul~erdem hat uns Herr Dr. R. lqEv (Karlsruhe) freundlieherweise eine Probe der hoehgereinigten Substanz zum Vergleieh zur Verfiigung gestellt. *])77.

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The use of basic Fuchsin in Plant Anatomy

Cited by 4 publications

References 0 publications

Towards portable MRI in the plant sciences

Towards portable MRI in the plant sciences

Ontogeny and Correlative Relationships of the Primary Thickening Meristem in Four‐o'clock Plants (Nyctaginaceae) Maintained Under Long and Short Photoperiods

Untersuchungen zur biologischen Aktivität der Phenylborsäure

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