The Use of Beem Capsules in the Preparation of Fresh Frozen Cryostat Sections

Av, Fasano; Pb, Berendsen; Labriola, Anna R.

doi:10.3109/10520297409116939

Stain Technology

1974

DOI: 10.3109/10520297409116939

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The Use of Beem Capsules in the Preparation of Fresh Frozen Cryostat Sections

Fasano Av

Berendsen Pb

Anna R. Labriola

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1976

1991

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A new integrated concept for the improved preparation of sections of fresh or frozen tissue for light microscope histochemistry

Fink

1986

Histochemistry

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In order to obtain thin sections of plant tissues which combined good morphological preservation and the preservation of the substances and enzyme activities in the tissues, a concept of section preparation by external stabilization was developed. The main components are as follows: appropriate supporting medium; surface coating before each sectioning process, the coating being either non-permanent, permanent, or semi-permanent; suitable techniques for affixing the coated sections to the slides using either pressure-sensitive adhesive or solvent-based adhesive; and mounting media with defined refractive indices (preferably UV-curable, water-soluble monomers). By this approach, sections exhibiting excellent morphological and physiological preservation were obtained using either a cryostat at -30 degrees C or a rotary microtome at room temperature.

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A new integrated concept for the improved preparation of sections of fresh or frozen tissue for light microscope histochemistry

Fink

1986

Histochemistry

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The use of gelatin capsules in mounting organ culture explants for cryostat sectioning

Klann

Fischer

1976

Tca Manual

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Flash Freezing of Tissues for Immunohistochemical and Biochemical Reactions

Stever¹

1991

Journal of Histotechnology

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The Use of Beem Capsules in the Preparation of Fresh Frozen Cryostat Sections

Cited by 4 publications

References 0 publications

A new integrated concept for the improved preparation of sections of fresh or frozen tissue for light microscope histochemistry

A new integrated concept for the improved preparation of sections of fresh or frozen tissue for light microscope histochemistry

The use of gelatin capsules in mounting organ culture explants for cryostat sectioning

Flash Freezing of Tissues for Immunohistochemical and Biochemical Reactions

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