Introduction: This study aimed to investigate the interfacial adaptation of CeraSeal and Bio-C Sealer in comparison to AH Plus using scanning electron microscope (SEM). Additionally, to compare penetration depth of CeraSeal and Bio-C Sealer with AH Plus using confocal laser scanning microscopy (CLSM).Materials and Methods: Sixty-six extracted single rooted human premolars were collected and randomly divided into three groups according to sealer type AH Plus, Bio-C Sealer and CeraSeal using single cone obturation technique. Then each group was divided into 2 subgroups (n=11), one of the subdivided groups was obturated, then samples were cut longitudinally to be evaluated for interfacial adaptation using SEM at magnification 500x. For the other 33 samples, the sealer was mixed with Rhodamine B dye obturated, then the samples were cut horizontally at (3, 6 and 9 mm) to be evaluated for depth of penetration using CLSM.Results: For interfacial adaptation, AH Plus showed better adaptation without statistically significant difference between them. The maximum depth of penetration, Bio-C Sealer showed the maximum penetration, without statistically significant difference between them. While for penetration %, there was a statistically significant difference between the three sealers, with the Bio-C showed the best penetration percentage.
Conclusions:Within the limitations of this study, it was concluded that AH Plus showed the best insignificant sealer adaptation to the root canal wall, while Bio-C sealer showed the most optimal dentinal tubular penetration of the tested sealers.